Sucrose Secreted by the Engineered Cyanobacterium and Its Fermentability

doi:10.1007/s11802-016-3007-8

QIBEBT-IR > 微生物代谢工程研究组

	Sucrose Secreted by the Engineered Cyanobacterium and Its Fermentability
	Duan Yangkai 1,2; Luo Quan1 ; Liang Feiyan1,2 ; Lu Xuefeng 1
	2016-10-01
发表期刊	JOURNAL OF OCEAN UNIVERSITY OF CHINA
卷号	15 期号:5 页码:890-896
摘要	The unicellular cyanobacterium, Synechococcus elongatus PCC 7942 (Syn7942), synthesizes sucrose as the only compatible solute under salt stress. A series of engineered Syn7942 strains for sucrose production were constructed. The overexpression of the native sps (encoding a natively fused protein of sucrose phosphate synthase SPS and sucrose phosphate phosphatase SPP) in Syn7942 wild type caused a 93% improvement of sucrose productivity. The strain FL130 co-overexpressing sps and cscB (encoding a sucrose transporter) exhibited a 74% higher extracellular sucrose production than that overexpressing cscB only. Both results showed the significant improvement of sucrose productivity by the double functional protein SPS-SPP. Afterwards, FL130 was cultivated under a modified condition, and the cell-free culture medium containing 1.5 g L-1 sucrose was pre-treated with an acid hydrolysis technique. Cultivated with the neutralized hydrolysates as the starting media, two widely used microorganisms, Escherichia coli and Saccharomyces cerevisiae, showed a comparable growth with that in the control media supplemented with glucose. These results clearly demonstrated that the cell-free culture of sucrose-secreting cyanobacteria can be applied as starting media in microbial cultivation.
文章类型	Article
关键词	Pre-treatment Salt Stress Sps Sucrose Synechococcus Elongatus Pcc 7942
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1007/s11802-016-3007-8
收录类别	SCI
语种	英语
WOS研究方向	Oceanography
项目资助者	Joint Sino-German Research Project(GZ984) ; Excellent Youth Award of the Shandong Natural Science Foundation(JQ201306) ; Shandong Taishan Scholarship
WOS类目	Oceanography
WOS记录号	WOS:000387574800018
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9096
专题	微生物代谢工程研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Duan Yangkai,Luo Quan,Liang Feiyan,et al. Sucrose Secreted by the Engineered Cyanobacterium and Its Fermentability[J]. JOURNAL OF OCEAN UNIVERSITY OF CHINA,2016,15(5):890-896.
APA	Duan Yangkai,Luo Quan,Liang Feiyan,&Lu Xuefeng.(2016).Sucrose Secreted by the Engineered Cyanobacterium and Its Fermentability.JOURNAL OF OCEAN UNIVERSITY OF CHINA,15(5),890-896.
MLA	Duan Yangkai,et al."Sucrose Secreted by the Engineered Cyanobacterium and Its Fermentability".JOURNAL OF OCEAN UNIVERSITY OF CHINA 15.5(2016):890-896.