A new strategy for strain improvement of Aurantiochytrium sp based on heavy-ions mutagenesis and synergistic effects of cold stress and inhibitors of enoyl-ACP reductase

doi:10.1016/j.enzmictec.2016.08.019

QIBEBT-IR > 代谢物组学研究组

	A new strategy for strain improvement of Aurantiochytrium sp based on heavy-ions mutagenesis and synergistic effects of cold stress and inhibitors of enoyl-ACP reductase
	Cheng Yu-rong 1,3,4; Sun Zhi-jie 1; Cui Gu-zhen 1,3; Song Xiaojin 1,3; Cui Qiu 1,2,3
	2016-11-01
发表期刊	ENZYME AND MICROBIAL TECHNOLOGY
卷号	93-94 页码:182-190
摘要	Developing a strain with high docosahexaenoic acid (DHA) yield and stable fermenting-performance is an imperative way to improve DHA production using Aurantiochytrium sp., a microorganism with two fatty acid synthesis pathways: polyketide synthase (PKS) pathway and Type I fatty acid synthase (FAS) pathway. This study investigated the growth and metabolism response of Aurantiochytrium sp. CGMCC 6208 to two inhibitors of enoyl-ACP reductase of Type II FAS pathway (isoniazid and triclosan), and proposed a method of screening high DHA yield Aurantiochytrium sp. strains with heavy ion mutagenesis and pre-selection by synergistic usage of cold stress (4 degrees C) and FAS inhibitors (triclosan and isoniazid). Results showed that (1) isoniazid and triclosan have positive effects on improving DHA level of cells; (2) mutants from irradiation dosage of 120 Gy yielded more DHA compared with cells from 40 Gy, 80 Gy treatment and wild type; (3) DHA contents of mutants pre-selected by inhibitors of enoyl-ACP reductase of Type II FAS pathway (isoniazid and triclosan)at 4 degrees C, were significantly higher than that of wild type; (4) compared to the wild type, the DHA productivity and yield of a mutant (T-99) obtained from Aurantiochytrium sp. CGMCC 6208 by the proposed method increased by 50% from 0.18 to 0.27 g/Lh and 30% from 21 to 27 g/L, respectively. In conclusion, this study developed a feasible method to screen Aurantiochytrium sp. with high DHA yield by a combination of heavy-ion mutagenesis and mutant-preselection by FAS inhibitors and cold stress. (C) 2016 Elsevier Inc. All rights reserved.
文章类型	Article
关键词	Aurantiochytrium Sp. Heavy-ions Mutagenesis Triclosan Isoniazid Coldstress
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.enzmictec.2016.08.019
关键词[WOS]	FATTY-ACID BIOSYNTHESIS ; DOCOSAHEXAENOIC ACID ; SCHIZOCHYTRIUM SP ; ARABIDOPSIS-THALIANA ; GAMMA-IRRADIATION ; LOW-TEMPERATURE ; OIL PRODUCTION ; MUTATIONS ; NANNOCHLOROPSIS ; OPTIMIZATION
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
项目资助者	National Natural Science Foundation of China(41306132 ; National High Technology Research and Development Program of China(2014AA021701) ; Qingdao Science and Technology Development Plan(14-2-4-69-jch) ; Qingdao Institute of Bioenergy and Bioprocess Technology Director Innovation Foundation for Young Scientists(QIBEBT-201503) ; 31500078)
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000386317700021
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9069
专题	代谢物组学研究组
作者单位	1.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao, Shandong, Peoples R China 3.Qingdao Engn Lab Single Cell Oil, Qingdao, Shandong, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Cheng Yu-rong,Sun Zhi-jie,Cui Gu-zhen,et al. A new strategy for strain improvement of Aurantiochytrium sp based on heavy-ions mutagenesis and synergistic effects of cold stress and inhibitors of enoyl-ACP reductase[J]. ENZYME AND MICROBIAL TECHNOLOGY,2016,93-94:182-190.
APA	Cheng Yu-rong,Sun Zhi-jie,Cui Gu-zhen,Song Xiaojin,&Cui Qiu.(2016).A new strategy for strain improvement of Aurantiochytrium sp based on heavy-ions mutagenesis and synergistic effects of cold stress and inhibitors of enoyl-ACP reductase.ENZYME AND MICROBIAL TECHNOLOGY,93-94,182-190.
MLA	Cheng Yu-rong,et al."A new strategy for strain improvement of Aurantiochytrium sp based on heavy-ions mutagenesis and synergistic effects of cold stress and inhibitors of enoyl-ACP reductase".ENZYME AND MICROBIAL TECHNOLOGY 93-94(2016):182-190.