Single cell stable isotope probing in microbiology using Raman microspectroscopy

doi:10.1016/j.copbio.2016.04.018

QIBEBT-IR > 单细胞中心组群

	Single cell stable isotope probing in microbiology using Raman microspectroscopy
	Wang, Yun1,2,3 ; Huang, Wei E.4; Cui, Li 5; Wagner, Michael 6
	2016-10-01
发表期刊	CURRENT OPINION IN BIOTECHNOLOGY
卷号	41 页码:34-42
摘要	Microbial communities are essential for most ecosystem processes and interact in highly complex ways with virtually all eukaryotes. Thus, a detailed understanding of the function of such communities is a fundamental prerequisite for microbial ecologists, applied microbiologists and microbiome researchers. Using single cell Raman microspectroscopy, biochemical fingerprints of individual microbial cells can be obtained in an externally label-free and non-destructive manner. If combined with stable isotope probing (SIP), Raman spectroscopy can directly reveal functions of single microorganisms in their natural habitat. This review provides an update on various SIP-approaches suitable for combination with different Raman scattering techniques and illustrates how single cell Raman SIP can be directly combined with the omics-centric analysis pipelines to investigate microbial communities.
文章类型	Review
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.copbio.2016.04.018
关键词[WOS]	IN-SITU HYBRIDIZATION ; SPECTROSCOPY ; MICROSCOPY ; MICROORGANISMS ; RNA ; MACROPHAGES ; COMBINATION ; POPULATIONS ; SCATTERING ; PHYLOGENY
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
项目资助者	EPSRC(EP/M002403/1) ; NERC(NE/M002934/1) ; BBSRC sLoLa(BB/M000265/1) ; NSFC(31400436) ; CAS(XDB15040100) ; ERC Advanced Grant (NITRICARE)(294343) ; Emerging Technologies Opportunity Program, Office of Science of the US Department of Energy(DE-AC02-05CH11231)
WOS类目	Biochemical Research Methods ; Biotechnology & Applied Microbiology
WOS记录号	WOS:000385899800007
引用统计	被引频次：156[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9068
专题	单细胞中心组群
作者单位	1.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, CAS Key Lab Biofuels, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Peoples R China 3.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Single Cell Ctr, Qingdao 266101, Peoples R China 4.Univ Oxford, Dept Engn Sci, Parks Rd, Oxford OX1 3PJ, England 5.Chinese Acad Sci, Inst Urban Environm, Key Lab Urban Environm & Hlth, Xiamen 361021, Peoples R China 6.Univ Vienna, Div Microbial Ecol, Dept Microbiol & Ecosyst Sci, Res Network Chem Meets Microbiol, A-1090 Vienna, Austria
推荐引用方式 GB/T 7714	Wang, Yun,Huang, Wei E.,Cui, Li,et al. Single cell stable isotope probing in microbiology using Raman microspectroscopy[J]. CURRENT OPINION IN BIOTECHNOLOGY,2016,41:34-42.
APA	Wang, Yun,Huang, Wei E.,Cui, Li,&Wagner, Michael.(2016).Single cell stable isotope probing in microbiology using Raman microspectroscopy.CURRENT OPINION IN BIOTECHNOLOGY,41,34-42.
MLA	Wang, Yun,et al."Single cell stable isotope probing in microbiology using Raman microspectroscopy".CURRENT OPINION IN BIOTECHNOLOGY 41(2016):34-42.