Effects of light intensity on metabolism and antioxidant defense in Haliotis discus hannai Ino

doi:10.1016/j.aquaculture.2016.08.010

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	Effects of light intensity on metabolism and antioxidant defense in Haliotis discus hannai Ino
	Gao Xiaolong 1,2; Li Xian 2; Li Meijie 3; Song Changbin 4; Liu Ying 2
	2016-12-01
发表期刊	AQUACULTURE
卷号	465 页码:78-87
摘要	We examined the effects of light intensity on the metabolism, antioxidant enzymes, and expression of related genes in Haliotis discus hannai Ino, to determine the optimal lighting conditions for aquaculture of these animals. Succinate dehydrogenase activity was significantly lower after 48 h in animals exposed to light intensities of 30 and 60 mu mol/m(2)/s, while lactate dehydrogenase activity and lactic acid content at 96 h were significantly higher compared with animals in the dark or exposed to 5 or 15 mu mol/m(2)/s (both P < 0.05). There was no significant difference in reactive oxygen species (ROS) contents between animals in the dark and those exposed to 5 or 15 mu mol/m(2)/s (P > 0.05) throughout the experiment. However, ROS contents under light intensities of 30 and 60 mu mol/m(2)/s peaked at 12 h, when expression levels of genes encoding catalase (CAT), thioredoxin peroxidase (TPX), sigma-glutathione-s-transferase (GST(S)), and mu-glutathione-s-transferase (GST(m)) also began to increase, and CAT and glutathione peroxidase (GP(X)) activities were significantly higher than in animals in the dark or exposed to 5 or 15 mu mol/m(2)/s (P < 0.05). After a further 12 h, the ROS content at 30 mu mol/m(2)/s began to decrease, and GPX activity and malondialdehyde (MDA) content also gradually decreased, followed by increases in ROS and MDA 48 h later, to reach respective peaks at 96 h. Gene expression levels of TPX, GST(S), GST(m), heat shock protein 70 (HSP70), and CAT began to decrease 72 h later, suggesting that excessive accumulation of ROS may have caused oxidative damage to the tissues. At a light intensity of 60 mu mol/m(2)/s, expression levels of TPX, GST(S), GST(m), and HSP70 genes, total antioxidant capacity, and GP(X) activity peaked at 48 h, and the ROS content was significantly higher than in any other group 48 h later (P < 0.05). However, gene expression levels of TPX, GST(S), GST(m), HSP70, HSP90, and CAT, and reduced glutathione content began to decrease, indicating that the antioxidant system had been unable to perform its normal physiological functions to withstand the long-term stress of adverse environmental factors. These results suggest that the light intensity should be controlled at 5-15 mu mol/m(2)/s during H. discus hannai aquaculture, to maintain the organism's normal physiological metabolism.
文章类型	Article
关键词	Haliotis Discus Hannai Ino Light Intensity Antioxidant Enzyme Heat Shock Protein Real-time Quantitative Pcr
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.aquaculture.2016.08.010
关键词[WOS]	MESSENGER-RNA EXPRESSION ; HEAT-SHOCK-PROTEIN ; GILTHEAD SEA BREAM ; OXIDATIVE STRESS ; DISK ABALONE ; VITAMIN-E ; HEAT-SHOCK-PROTEIN-90 GENE ; MOLECULAR CHARACTERIZATION ; LACTATE CONCENTRATIONS ; LIPID-PEROXIDATION
收录类别	SCI
语种	英语
WOS研究方向	Fisheries ; Marine & Freshwater Biology
项目资助者	earmarked fund for the Modern Agro-industry Technology Research System(CARS-48) ; National Natural Science Foundation of China(31402283 ; 41306152)
WOS类目	Fisheries ; Marine & Freshwater Biology
WOS记录号	WOS:000385372200011
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/9063
专题	其他文献
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Dalian Ocean Univ, Dalian 116023, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc, Qingdao 266101, Peoples R China 4.Chinese Acad Sci, Inst Semicond, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Gao Xiaolong,Li Xian,Li Meijie,et al. Effects of light intensity on metabolism and antioxidant defense in Haliotis discus hannai Ino[J]. AQUACULTURE,2016,465:78-87.
APA	Gao Xiaolong,Li Xian,Li Meijie,Song Changbin,&Liu Ying.(2016).Effects of light intensity on metabolism and antioxidant defense in Haliotis discus hannai Ino.AQUACULTURE,465,78-87.
MLA	Gao Xiaolong,et al."Effects of light intensity on metabolism and antioxidant defense in Haliotis discus hannai Ino".AQUACULTURE 465(2016):78-87.