Putative methyltransferase LaeA and transcription factor CreA are necessary for proper asexual development and controlling secondary metabolic gene cluster expression

doi:10.1016/j.fgb.2016.07.004

QIBEBT-IR > 分子微生物工程研究组

	Putative methyltransferase LaeA and transcription factor CreA are necessary for proper asexual development and controlling secondary metabolic gene cluster expression
	Zhang, Xiujun 1,2,3; Zhu, Yingying 1,2; Bao, Longfei 1,2; Gao, Liwei 1,2; Yao, Guangshan 1,2; Li, Yanan 1,2,3; Yang, Zhifeng 4; Li, Zhonghai 1,2; Zhong, Yaohua 1,2; Li, Fuli5 ; Yin, Heng 6; Qu, Yinbo 1,2; Qin, Yuqi 1,2,3
	2016-09-01
发表期刊	FUNGAL GENETICS AND BIOLOGY
卷号	94 页码:32-46
摘要	The morphological development of fungi is a complex process and is often coupled with secondary metabolite production. In this study, we assessed the function of putative methyltransferase LaeA and transcription factor CreA in controlling asexual development and secondary metabolic gene cluster expression in Penicillium oxalicum. The deletion of laeA (Delta laeA) impaired the conidiation in P. oxalicum, with a downregulated expression of brlA. Overexpression of P. oxalicum brlA in Delta laeA could upregulate brlA and abaA remarkably, but could not rescue the conidiation defect; therefore, brlA and abaA expression were necessary but not sufficient for conidiation. Deletion of creA in Delta laeA background (Delta laeA Delta creA) blocked conidiation with a white fluffy phenotype. Nutrient-rich medium could not rescue developmental defects in Delta laeA Delta creA mutant but could rescue defects in Delta laeA. Expression of 10 genes, namely, albA/wA, abrB/yA, arpA, aygA, arpA-like, arpB, arpB-like, rodA, rodA-like, and rodB, for pigmentation and spore wall protein genes was silenced in Delta laeA Delta creA, whereas only six of them were downregulated in Delta laeA. Among the 28 secondary metabolism gene clusters in P. oxalicum, four secondary metabolism gene clusters were silenced in Delta laeA and two were also silenced in Delta brlA mutant. A total of 10 physically linked and coregulated genes were distributed over five chromosomes in Delta laeA. Six of these genes were located in subtelomeric regions, thus demonstrating a positional bias for LaeA-regulated clusters toward subtelomeric regions. All of silenced clusters located in subtelomeric regions were derepressed in Delta laeA Delta creA, hence showing that lack of CreA could remediate the repression of gene clusters in Delta laeA background. Results show that both putative methyltransferase LaeA and transcription factor CreA are necessary for proper asexual development and controlling secondary metabolic gene cluster expression. (C) 2016 Elsevier Inc. All rights reserved.
文章类型	Article
关键词	Penicillium Oxalicum Laea Crea Asexual Development Secondary Metabolism
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.fgb.2016.07.004
关键词[WOS]	CARBON CATABOLITE REPRESSION ; ASPERGILLUS-NIDULANS ; PENICILLIUM-CHRYSOGENUM ; TRICHODERMA-REESEI ; FILAMENTOUS FUNGI ; CONIDIOPHORE DEVELOPMENT ; CHEMICAL DIVERSITY ; ROQUEFORTINE C ; BIOSYNTHESIS ; FUMIGATUS
收录类别	SCI
语种	英语
WOS研究方向	Genetics & Heredity ; Mycology
项目资助者	National Natural Sciences Foundation of China(31370086) ; National High Technology Research and Development Program(2012AA022203B) ; Foundation of Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences(CASKLB201509)
WOS类目	Genetics & Heredity ; Mycology
WOS记录号	WOS:000381242900005
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/8500
专题	分子微生物工程研究组
作者单位	1.Shandong Univ, Natl Glycoengn Res Ctr, Jinan 250100, Peoples R China 2.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China 3.Shandong Univ, Shandong Prov Key Lab Carbohydrate Chem & Glycobi, Jinan 250100, Peoples R China 4.Shandong Univ, Sch Math, Jinan 250100, Peoples R China 5.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 6.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xiujun,Zhu, Yingying,Bao, Longfei,et al. Putative methyltransferase LaeA and transcription factor CreA are necessary for proper asexual development and controlling secondary metabolic gene cluster expression[J]. FUNGAL GENETICS AND BIOLOGY,2016,94:32-46.
APA	Zhang, Xiujun.,Zhu, Yingying.,Bao, Longfei.,Gao, Liwei.,Yao, Guangshan.,...&Qin, Yuqi.(2016).Putative methyltransferase LaeA and transcription factor CreA are necessary for proper asexual development and controlling secondary metabolic gene cluster expression.FUNGAL GENETICS AND BIOLOGY,94,32-46.
MLA	Zhang, Xiujun,et al."Putative methyltransferase LaeA and transcription factor CreA are necessary for proper asexual development and controlling secondary metabolic gene cluster expression".FUNGAL GENETICS AND BIOLOGY 94(2016):32-46.