In depth understanding the molecular response to the enhanced secretion of fatty acids in Saccharomyces cerevisiae due to one-step gene deletion of acyl-CoA synthetases

doi:10.1016/j.procbio.2016.05.017

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	In depth understanding the molecular response to the enhanced secretion of fatty acids in Saccharomyces cerevisiae due to one-step gene deletion of acyl-CoA synthetases
	Fang, Fang 1; Dai, Bingye 2; Zhao, Guang 1; Zhao, Hua 3; Sun, Chao1 ; Liu, Hui1 ; Xian, Mo1
	2016-09-01
发表期刊	PROCESS BIOCHEMISTRY
卷号	51 期号:9 页码:1162-1174
摘要	Considering of the possible application of fatty acids secretion in industrial production process for reducing extraction costs, the effects of enhanced fatty acids secretion by knocking out acyl-CoA synthetases on the global regulation, cellular metabolism were investigated through integrated omics analysis. Firstly, functional category analysis of differential expression genes showed that most of the genes were associated with the cell cycle and biology process such as cellular component biogenesis, cell wall organization and so on. Secondly, the decreased mRNA levels of Tdh, Eno and Pgk1 in glycolysis in faa mutant YB526 were confirmed by the differential protein analysis. As described in previous reports, the decreased protein levels of Tdh, Eno and Pgk1 were resulted from the increase of 14-3-3 protein activity. However, the increased level of 14-3-3 proteins could not improve the biosynthesis of ergosterol in the acyl-CoA deficiency strain YB526 due to the toxic effects of fatty acids. Thirdly, the decrease of ergosterol and ceramide contents in cell envelopes can improve the permeation of membrane and fatty acids secretion in faa mutant YB526. This comprehensive omics analysis could be helpful in the rational design of robust yeasts for the production of free fatty acids or other biobased chemicals. (C) 2016 Elsevier Ltd. All rights reserved.
文章类型	Article
关键词	Fatty Acids Cell Envelope Biobased Chemicals Integrated Omics Analysis Cellular Response Saccharomyces Cerevisiae
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Technology
DOI	10.1016/j.procbio.2016.05.017
关键词[WOS]	SP PCC 6803 ; ESCHERICHIA-COLI ; BACILLUS-SUBTILIS ; INTEGRATIVE ANALYSIS ; PROTEOME ANALYSIS ; ANALYSIS REVEALS ; YEAST ; TRANSCRIPTOME ; METABOLISM ; RESISTANCE
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering
项目资助者	National Natural Science Foundation(21106170 ; Sci-Tech development project of Qingdao(12-4-1-45-nsh) ; 21202179 ; 21376255)
WOS类目	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
WOS记录号	WOS:000382349500007
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/8489
专题	生物基材料组群
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China 2.China Rural Technol Dev Ctr, Beijing 100045, Peoples R China 3.Tianjin Univ Sci & Technol, Coll Biotechnol, Tianjin 300457, Peoples R China
推荐引用方式 GB/T 7714	Fang, Fang,Dai, Bingye,Zhao, Guang,et al. In depth understanding the molecular response to the enhanced secretion of fatty acids in Saccharomyces cerevisiae due to one-step gene deletion of acyl-CoA synthetases[J]. PROCESS BIOCHEMISTRY,2016,51(9):1162-1174.
APA	Fang, Fang.,Dai, Bingye.,Zhao, Guang.,Zhao, Hua.,Sun, Chao.,...&Xian, Mo.(2016).In depth understanding the molecular response to the enhanced secretion of fatty acids in Saccharomyces cerevisiae due to one-step gene deletion of acyl-CoA synthetases.PROCESS BIOCHEMISTRY,51(9),1162-1174.
MLA	Fang, Fang,et al."In depth understanding the molecular response to the enhanced secretion of fatty acids in Saccharomyces cerevisiae due to one-step gene deletion of acyl-CoA synthetases".PROCESS BIOCHEMISTRY 51.9(2016):1162-1174.