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Efficacy of head space solid-phase microextraction coupled to gas chromatography-mass spectrometry method for determination of the trace extracellular hydrocarbons of cyanobacteria
Guan, Wenna1; Zhu, Tao2; Wang, Yuejie3; Zhang, Zhongyi3; Jin, Zhao4; Wang, Cong1; Bai, Fali1
2016-09-01
Source PublicationJOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume1029Pages:113-120
AbstractHydrocarbons are widespread in cyanobacteria, and the biochemical synthetic pathways were recently identified. Intracellular fatty alka(e)nes of cyanobacteria have been detected by liquid-liquid extraction (LLE) coupled to gas chromatography-mass spectrometry (GC/MS). However, whether fatty alka(e)nes can be released to cyanobacterial culture media remains to be clarified. This work develops a sensitive method for analyzing the trace level of extracellular hydrocarbons in cyanobacterial culture media by head space solid-phase microextraction (HS-SPME) coupled to GC/MS. Headspace (HS) extraction mode using polydimethylsiloxane fiber to extract for 30 min at 50 degrees C was employed as the optimal extraction conditions. Five cyanobacterial fatty alka(e)nes analogs including pentadecene (C15:1), pentadecane (C15:0), heptadecene (C17:1), heptadecane (C17:0), nonadecane (C19:0) were analyzed, and the data obtained from HS-SPME-GC/MS method were quantified using internal standard peak area comparisons. Limits of detection (LOD), limits of quantitation (LOQ), linear dynamic range, precisions (RSD) and recovery for the analysis of extracellular fatty alka(e)nes of cyanobacteria by HS-SPME-GC/MS were evaluated. The LODs limits of detection (S/N = 3) varied from 10 to 21 ng L-1. The correlation coefficients (r) of the calibration curves ranged from 0.9873 to 0.9977 with a linearity from 0.1 to 50 mu g L-1. The RSD values were ranging from 7.8 to 14.0% and from 4.0 to 8.8% at 1.0 mu g L-1 and 10.0 mu g L-1 standard solutions, respectively. Comparative analysis of extracellular fatty alka(e)nes in the culture media of model cyanobacteria Synechocystis sp. PCC 6803 demonstrated that sensitivity of HS-SPME-GC/MS method was significantly higher than LLE method. Finally, we found that heptadecane can be released into the culture media of Synechocystis sp. PCC 6803 at the later growth period. (C) 2016 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordSolid-phase Microextraction Cyanobacteria Extracellular Hydrocarbons Gas Chromatography-mass Spectrometry (Gc-ms)
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.jchromb.2016.06.050
WOS KeywordALGAE ; ALKA(E)NES ; ALDEHYDES ; PRODUCTS ; FORMATE ; ALKANES
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Chemistry
Funding OrganizationNational Natural Science Foundation of China(21305149 ; 31200001)
WOS SubjectBiochemical Research Methods ; Chemistry, Analytical
WOS IDWOS:000381651900016
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/8484
Collection公共实验室
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, 189 Songling Rd, Qingdao 266101, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Key Lab Biofuels, 189 Songling Rd, Qingdao 266101, Peoples R China
3.Qingdao Univ, Coll Chem Sci & Engn, 308 Ningxia Rd, Qingdao 266071, Peoples R China
4.Qingdao Zhenzheng Analyt Instruments Co Ltd, 89 Changcheng Rd, Qingdao 266109, Peoples R China
Recommended Citation
GB/T 7714
Guan, Wenna,Zhu, Tao,Wang, Yuejie,et al. Efficacy of head space solid-phase microextraction coupled to gas chromatography-mass spectrometry method for determination of the trace extracellular hydrocarbons of cyanobacteria[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2016,1029:113-120.
APA Guan, Wenna.,Zhu, Tao.,Wang, Yuejie.,Zhang, Zhongyi.,Jin, Zhao.,...&Bai, Fali.(2016).Efficacy of head space solid-phase microextraction coupled to gas chromatography-mass spectrometry method for determination of the trace extracellular hydrocarbons of cyanobacteria.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1029,113-120.
MLA Guan, Wenna,et al."Efficacy of head space solid-phase microextraction coupled to gas chromatography-mass spectrometry method for determination of the trace extracellular hydrocarbons of cyanobacteria".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1029(2016):113-120.
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