Efficacy of head space solid-phase microextraction coupled to gas chromatography-mass spectrometry method for determination of the trace extracellular hydrocarbons of cyanobacteria | |
Guan, Wenna1; Zhu, Tao2; Wang, Yuejie3; Zhang, Zhongyi3; Jin, Zhao4; Wang, Cong1; Bai, Fali1 | |
2016-09-01 | |
发表期刊 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES |
卷号 | 1029页码:113-120 |
摘要 | Hydrocarbons are widespread in cyanobacteria, and the biochemical synthetic pathways were recently identified. Intracellular fatty alka(e)nes of cyanobacteria have been detected by liquid-liquid extraction (LLE) coupled to gas chromatography-mass spectrometry (GC/MS). However, whether fatty alka(e)nes can be released to cyanobacterial culture media remains to be clarified. This work develops a sensitive method for analyzing the trace level of extracellular hydrocarbons in cyanobacterial culture media by head space solid-phase microextraction (HS-SPME) coupled to GC/MS. Headspace (HS) extraction mode using polydimethylsiloxane fiber to extract for 30 min at 50 degrees C was employed as the optimal extraction conditions. Five cyanobacterial fatty alka(e)nes analogs including pentadecene (C15:1), pentadecane (C15:0), heptadecene (C17:1), heptadecane (C17:0), nonadecane (C19:0) were analyzed, and the data obtained from HS-SPME-GC/MS method were quantified using internal standard peak area comparisons. Limits of detection (LOD), limits of quantitation (LOQ), linear dynamic range, precisions (RSD) and recovery for the analysis of extracellular fatty alka(e)nes of cyanobacteria by HS-SPME-GC/MS were evaluated. The LODs limits of detection (S/N = 3) varied from 10 to 21 ng L-1. The correlation coefficients (r) of the calibration curves ranged from 0.9873 to 0.9977 with a linearity from 0.1 to 50 mu g L-1. The RSD values were ranging from 7.8 to 14.0% and from 4.0 to 8.8% at 1.0 mu g L-1 and 10.0 mu g L-1 standard solutions, respectively. Comparative analysis of extracellular fatty alka(e)nes in the culture media of model cyanobacteria Synechocystis sp. PCC 6803 demonstrated that sensitivity of HS-SPME-GC/MS method was significantly higher than LLE method. Finally, we found that heptadecane can be released into the culture media of Synechocystis sp. PCC 6803 at the later growth period. (C) 2016 Elsevier B.V. All rights reserved. |
文章类型 | Article |
关键词 | Solid-phase Microextraction Cyanobacteria Extracellular Hydrocarbons Gas Chromatography-mass Spectrometry (Gc-ms) |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
DOI | 10.1016/j.jchromb.2016.06.050 |
关键词[WOS] | ALGAE ; ALKA(E)NES ; ALDEHYDES ; PRODUCTS ; FORMATE ; ALKANES |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
项目资助者 | National Natural Science Foundation of China(21305149 ; 31200001) |
WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
WOS记录号 | WOS:000381651900016 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qibebt.ac.cn/handle/337004/8484 |
专题 | 公共实验室 |
作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, 189 Songling Rd, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Key Lab Biofuels, 189 Songling Rd, Qingdao 266101, Peoples R China 3.Qingdao Univ, Coll Chem Sci & Engn, 308 Ningxia Rd, Qingdao 266071, Peoples R China 4.Qingdao Zhenzheng Analyt Instruments Co Ltd, 89 Changcheng Rd, Qingdao 266109, Peoples R China |
推荐引用方式 GB/T 7714 | Guan, Wenna,Zhu, Tao,Wang, Yuejie,et al. Efficacy of head space solid-phase microextraction coupled to gas chromatography-mass spectrometry method for determination of the trace extracellular hydrocarbons of cyanobacteria[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2016,1029:113-120. |
APA | Guan, Wenna.,Zhu, Tao.,Wang, Yuejie.,Zhang, Zhongyi.,Jin, Zhao.,...&Bai, Fali.(2016).Efficacy of head space solid-phase microextraction coupled to gas chromatography-mass spectrometry method for determination of the trace extracellular hydrocarbons of cyanobacteria.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1029,113-120. |
MLA | Guan, Wenna,et al."Efficacy of head space solid-phase microextraction coupled to gas chromatography-mass spectrometry method for determination of the trace extracellular hydrocarbons of cyanobacteria".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1029(2016):113-120. |
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