中国科学院青岛生物能源与过程研究所机构知识库
Advanced  
QIBEBT-IR  > 仿真与模拟团队  > 期刊论文
Title:
Cellulose Chain Binding Free Energy Drives the Processive Move of Cellulases on the Cellulose Surface
Author: Wang, Yefei1,2; Zhang, Shujun1,2; Song, Xiangfei1,2; Yao, Lishan1,2
Source: BIOTECHNOLOGY AND BIOENGINEERING
Issued Date: 2016-09-01
Volume: 113, Issue:9, Pages:1873-1880
Keyword: Cel7B ; Trichoderma reesei ; processivity ; free energy calculations
DOI: 10.1002/bit.25970
DOC Type: Article
English Abstract: Processivity is essential for cellulases in their catalysis of cellulose hydrolysis. But what drives the processive move is not well understood. In this work, we use Trichoderma reesei Cel7B as a model system and show that its processivity is directly correlated to the binding free energy difference of a cellulose chain occupying the binding sites -7 to +2 and that occupying sites -7 to -1. Several mutants that have stronger interactions with glycosyl units in sites +1 and +2 than the wild type enzyme show higher processivity. The results suggest that after the release of the product cellobiose located in sites +1 and +2, the enzyme pulls the cellulose chain to fill the vacant sites, which propels its processive move on the cellulose surface. (C) 2016 Wiley Periodicals, Inc.
WOS Headings: Science & Technology ; Life Sciences & Biomedicine
WOS Subject: Biotechnology & Applied Microbiology
WOS Subject Extended: Biotechnology & Applied Microbiology
WOS Keyword Plus: TRICHODERMA-REESEI CELLOBIOHYDROLASES ; AROMATIC-CARBOHYDRATE INTERACTIONS ; PARTICLE MESH EWALD ; MOLECULAR SIMULATION ; CRYSTALLINE CELLULOSE ; GLYCOSIDE HYDROLASES ; AUTOMATED DOCKING ; STRUCTURAL BASIS ; ENDOGLUCANASE-I ; ENZYMES
Indexed Type: SCI
Funder: National Natural Science Foundation of China(21173247 ; Qingdao Institute of Bioenergy and Bioprocess Technology Director Innovation Foundation(QIBEBT-DIFYS-201504) ; 31270785 ; 31400061)
Language: 英语
WOS ID: WOS:000384751500004
Citation statistics:
Content Type: 期刊论文
URI: http://ir.qibebt.ac.cn/handle/337004/8479
Appears in Collections:仿真与模拟团队_期刊论文

Files in This Item:

There are no files associated with this item.


description.institution: 1.Chinese Acad Sci, Shandong Prov Key Lab Synthet Biol, Qingdao, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biofuels, Qingdao 266061, Peoples R China

Recommended Citation:
Wang, Yefei,Zhang, Shujun,Song, Xiangfei,et al. Cellulose Chain Binding Free Energy Drives the Processive Move of Cellulases on the Cellulose Surface[J]. BIOTECHNOLOGY AND BIOENGINEERING,2016,113(9):1873-1880.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, Yefei]'s Articles
[Zhang, Shujun]'s Articles
[Song, Xiangfei]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, Yefei]‘s Articles
[Zhang, Shujun]‘s Articles
[Song, Xiangfei]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace