Cellulose Chain Binding Free Energy Drives the Processive Move of Cellulases on the Cellulose Surface

doi:10.1002/bit.25970

QIBEBT-IR > 蛋白质设计研究组

	Cellulose Chain Binding Free Energy Drives the Processive Move of Cellulases on the Cellulose Surface
	Wang, Yefei 1,2; Zhang, Shujun 1,2; Song, Xiangfei 1,2; Yao, Lishan 1,2
	2016-09-01
发表期刊	BIOTECHNOLOGY AND BIOENGINEERING
卷号	113 期号:9 页码:1873-1880
摘要	Processivity is essential for cellulases in their catalysis of cellulose hydrolysis. But what drives the processive move is not well understood. In this work, we use Trichoderma reesei Cel7B as a model system and show that its processivity is directly correlated to the binding free energy difference of a cellulose chain occupying the binding sites -7 to +2 and that occupying sites -7 to -1. Several mutants that have stronger interactions with glycosyl units in sites +1 and +2 than the wild type enzyme show higher processivity. The results suggest that after the release of the product cellobiose located in sites +1 and +2, the enzyme pulls the cellulose chain to fill the vacant sites, which propels its processive move on the cellulose surface. (C) 2016 Wiley Periodicals, Inc.
文章类型	Article
关键词	Cel7b Trichoderma Reesei Processivity Free Energy Calculations
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1002/bit.25970
关键词[WOS]	TRICHODERMA-REESEI CELLOBIOHYDROLASES ; AROMATIC-CARBOHYDRATE INTERACTIONS ; PARTICLE MESH EWALD ; MOLECULAR SIMULATION ; CRYSTALLINE CELLULOSE ; GLYCOSIDE HYDROLASES ; AUTOMATED DOCKING ; STRUCTURAL BASIS ; ENDOGLUCANASE-I ; ENZYMES
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
项目资助者	National Natural Science Foundation of China(21173247 ; Qingdao Institute of Bioenergy and Bioprocess Technology Director Innovation Foundation(QIBEBT-DIFYS-201504) ; 31270785 ; 31400061)
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000384751500004
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/8479
专题	蛋白质设计研究组
作者单位	1.Chinese Acad Sci, Shandong Prov Key Lab Synthet Biol, Qingdao, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biofuels, Qingdao 266061, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yefei,Zhang, Shujun,Song, Xiangfei,et al. Cellulose Chain Binding Free Energy Drives the Processive Move of Cellulases on the Cellulose Surface[J]. BIOTECHNOLOGY AND BIOENGINEERING,2016,113(9):1873-1880.
APA	Wang, Yefei,Zhang, Shujun,Song, Xiangfei,&Yao, Lishan.(2016).Cellulose Chain Binding Free Energy Drives the Processive Move of Cellulases on the Cellulose Surface.BIOTECHNOLOGY AND BIOENGINEERING,113(9),1873-1880.
MLA	Wang, Yefei,et al."Cellulose Chain Binding Free Energy Drives the Processive Move of Cellulases on the Cellulose Surface".BIOTECHNOLOGY AND BIOENGINEERING 113.9(2016):1873-1880.