QIBEBT-IR  > 蛋白质设计研究组
Cellulose Chain Binding Free Energy Drives the Processive Move of Cellulases on the Cellulose Surface
Wang, Yefei1,2; Zhang, Shujun1,2; Song, Xiangfei1,2; Yao, Lishan1,2
2016-09-01
发表期刊BIOTECHNOLOGY AND BIOENGINEERING
卷号113期号:9页码:1873-1880
摘要Processivity is essential for cellulases in their catalysis of cellulose hydrolysis. But what drives the processive move is not well understood. In this work, we use Trichoderma reesei Cel7B as a model system and show that its processivity is directly correlated to the binding free energy difference of a cellulose chain occupying the binding sites -7 to +2 and that occupying sites -7 to -1. Several mutants that have stronger interactions with glycosyl units in sites +1 and +2 than the wild type enzyme show higher processivity. The results suggest that after the release of the product cellobiose located in sites +1 and +2, the enzyme pulls the cellulose chain to fill the vacant sites, which propels its processive move on the cellulose surface. (C) 2016 Wiley Periodicals, Inc.
文章类型Article
关键词Cel7b Trichoderma Reesei Processivity Free Energy Calculations
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1002/bit.25970
关键词[WOS]TRICHODERMA-REESEI CELLOBIOHYDROLASES ; AROMATIC-CARBOHYDRATE INTERACTIONS ; PARTICLE MESH EWALD ; MOLECULAR SIMULATION ; CRYSTALLINE CELLULOSE ; GLYCOSIDE HYDROLASES ; AUTOMATED DOCKING ; STRUCTURAL BASIS ; ENDOGLUCANASE-I ; ENZYMES
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology
项目资助者National Natural Science Foundation of China(21173247 ; Qingdao Institute of Bioenergy and Bioprocess Technology Director Innovation Foundation(QIBEBT-DIFYS-201504) ; 31270785 ; 31400061)
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000384751500004
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/8479
专题蛋白质设计研究组
作者单位1.Chinese Acad Sci, Shandong Prov Key Lab Synthet Biol, Qingdao, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biofuels, Qingdao 266061, Peoples R China
推荐引用方式
GB/T 7714
Wang, Yefei,Zhang, Shujun,Song, Xiangfei,et al. Cellulose Chain Binding Free Energy Drives the Processive Move of Cellulases on the Cellulose Surface[J]. BIOTECHNOLOGY AND BIOENGINEERING,2016,113(9):1873-1880.
APA Wang, Yefei,Zhang, Shujun,Song, Xiangfei,&Yao, Lishan.(2016).Cellulose Chain Binding Free Energy Drives the Processive Move of Cellulases on the Cellulose Surface.BIOTECHNOLOGY AND BIOENGINEERING,113(9),1873-1880.
MLA Wang, Yefei,et al."Cellulose Chain Binding Free Energy Drives the Processive Move of Cellulases on the Cellulose Surface".BIOTECHNOLOGY AND BIOENGINEERING 113.9(2016):1873-1880.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Yefei]的文章
[Zhang, Shujun]的文章
[Song, Xiangfei]的文章
百度学术
百度学术中相似的文章
[Wang, Yefei]的文章
[Zhang, Shujun]的文章
[Song, Xiangfei]的文章
必应学术
必应学术中相似的文章
[Wang, Yefei]的文章
[Zhang, Shujun]的文章
[Song, Xiangfei]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。