The potential of Synechococcus elongatus UTEX 2973 for sugar feedstock production

doi:10.1007/s00253-016-7510-z

QIBEBT-IR > 微生物代谢工程研究组

	The potential of Synechococcus elongatus UTEX 2973 for sugar feedstock production
	Song, Kuo 1,2; Tan, Xiaoming 1; Liang, Yajing 1; Lu, Xuefeng 1
	2016-09-01
发表期刊	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷号	100 期号:18 页码:7865-7875
摘要	It is important to obtain abundant sugar feedstocks economically and sustainably for bio-fermentation industry, especially for producing cheap biofuels and biochemicals. Besides plant biomass, photosynthetic cyanobacteria have also been considered to be potential microbe candidates for sustainable production of carbohydrate feedstocks. As the fastest growing cyanobacterium reported so far, Synechococcus elongatus UTEX 2973 (Syn2973) might have huge potential for bioproduction. In this study, we explored the potentials of this strain as photo-bioreactors for sucrose and glycogen production. Under nitrogen-replete condition, Syn2973 could accumulate glycogen with a rate of 0.75 g L-1 day(-1) at the exponential phase and reach a glycogen content as high as 51 % of the dry cell weight (DCW) at the stationary phase. By introducing a sucrose transporter CscB, Syn2973 was endowed with an ability to secrete over 94 % sucrose out of cells under salt stress condition. The highest extracellular sucrose productivity reached 35.5 mg L-1 h(-1) for the Syn2973 strain expressing cscB, which contained the similar amounts of intracellular glycogen with the wild type. Potassium chloride was firstly proved to induce sucrose accumulation as well as sodium chloride in Syn2973. By semi-continuous culturing, 8.7 g L-1 sucrose was produced by the cscB-expressing strain of Syn2973 in 21 days. These results support that Syn2973 is a promising candidate with great potential for production of sugars.
文章类型	Article
关键词	Glycogen Sucrose Salt Stress Synechococcus Elongatus Utex 2973
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s00253-016-7510-z
关键词[WOS]	STRAIN PCC 7002 ; GLYCOGEN-SYNTHESIS ; CYANOBACTERIA ; NITROGEN ; SALT ; ACCLIMATION ; CONVERSION ; BIOFUELS ; SUCROSE ; ETHANOL
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
项目资助者	National Science Fund for Distinguished Young Scholars of China(31525002) ; Excellent Youth Award of the Shandong Natural Science Foundation(JQ201306) ; Shandong Taishan Scholarship ; National Science Foundation of China(31301018) ; State Oceanic Administration (SOA) Global Change and Air-Sea Interaction Program(GASI-03-01-02-05) ; Youth Innovation Promotion Association
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000382008000007
引用统计	被引频次：92[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/8421
专题	微生物代谢工程研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Shandong Prov Key Lab Synthet Biol, 189 Songling Rd, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Song, Kuo,Tan, Xiaoming,Liang, Yajing,et al. The potential of Synechococcus elongatus UTEX 2973 for sugar feedstock production[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2016,100(18):7865-7875.
APA	Song, Kuo,Tan, Xiaoming,Liang, Yajing,&Lu, Xuefeng.(2016).The potential of Synechococcus elongatus UTEX 2973 for sugar feedstock production.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,100(18),7865-7875.
MLA	Song, Kuo,et al."The potential of Synechococcus elongatus UTEX 2973 for sugar feedstock production".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 100.18(2016):7865-7875.