A sensitive acetylcholinesterase biosensor based on gold nanorods modified electrode for detection of organophosphate pesticide

doi:10.1016/j.talanta.2016.05.002

QIBEBT-IR > 环境资源化与水回用研究组

	A sensitive acetylcholinesterase biosensor based on gold nanorods modified electrode for detection of organophosphate pesticide
	Lang, Qiaolin 1,2,3; Han, Lei 1,2,3; Hou, Chuantao 1,2; Wang, Fei 1,2; Liu, Aihua 1,2
	2016-08-15
发表期刊	TALANTA
卷号	156 页码:34-41
摘要	A sensitive amperometric acetylcholinesterase (AChE) biosensor, based on gold nanorods (AuNRs), was developed for the detection of organophosphate pesticide. Compared with Au@Ag heterogeneous NRs, AuNRs exhibited excellent electrocatalytic properties, which can electrocatalytically oxidize thiocholine, the hydrolysate of acetylthiocholine chloride (ATCI) by AChE at +0.55 V (vs. SCE). The AChE/AuNRs/GCE biosensor was fabricated on basis of the inhibition of AChE activity by organophosphate pesticide. The biosensor could detect paraoxon in the linear range from 1 nM to 5 mu M and dimethoate in the linear range from 5 nM to 1 mu M, respectively. The detection limits of paraoxon and dimethoate were 0.7 nM and 3.9 nM, which were lower than the reported AChE biosensor. The proposed biosensor could restore to over 95% of its original current, which demonstrated the good reactivation. Moreover, the biosensor can be applicable to real water sample measurement. Thus, the biosensor exhibited low applied potential, high sensitivity and good stability, providing a promising tool for analysis of pesticides. (C) 2016 Elsevier B.V. All rights reserved.
文章类型	Article
关键词	Acetylcholinesterase Biosensor Gold Nanorods Au@ag Heterogeneous Nrs Organophosphate Pesticide
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1016/j.talanta.2016.05.002
关键词[WOS]	AG HETEROGENEOUS NANORODS ; GLUCOSE-OXIDASE ; MICROBIAL BIOSENSOR ; MULTIRESIDUE METHOD ; CARBON NANOTUBES ; NERVE AGENTS ; NANOPARTICLES ; SURFACE ; SENSOR ; HYDROLASE
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
项目资助者	National Natural Science Foundation of China(21275152 ; Natural Science Foundation of Shandong Province(ZR2015PB008) ; 21475144 ; 31500799)
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000378182900006
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/8336
专题	环境资源化与水回用研究组
作者单位	1.Qingdao Univ, Inst Biosensing, Qingdao 266071, Peoples R China 2.Qingdao Univ, Coll Chem & Chem Engn, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biosensing, 189 Songling Rd, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Lang, Qiaolin,Han, Lei,Hou, Chuantao,et al. A sensitive acetylcholinesterase biosensor based on gold nanorods modified electrode for detection of organophosphate pesticide[J]. TALANTA,2016,156:34-41.
APA	Lang, Qiaolin,Han, Lei,Hou, Chuantao,Wang, Fei,&Liu, Aihua.(2016).A sensitive acetylcholinesterase biosensor based on gold nanorods modified electrode for detection of organophosphate pesticide.TALANTA,156,34-41.
MLA	Lang, Qiaolin,et al."A sensitive acetylcholinesterase biosensor based on gold nanorods modified electrode for detection of organophosphate pesticide".TALANTA 156(2016):34-41.