Exploring the Synergy between Cellobiose Dehydrogenase from Phanerochaete chrysosporium and Cellulase from Trichoderma reesei

doi:10.3389/fmicb.2016.00620

QIBEBT-IR > 微生物代谢工程研究组

	Exploring the Synergy between Cellobiose Dehydrogenase from Phanerochaete chrysosporium and Cellulase from Trichoderma reesei
	Wang, Min 1,2; Lu, Xuefeng 1,3
	2016-04-29
发表期刊	FRONTIERS IN MICROBIOLOGY
卷号	7
摘要	Recent demands for the production of lignocellulose biofuels boosted research on cellulase. Hydrolysis efficiency and production cost of cellulase are two bottlenecks in "biomass to biofuels" process. The Trichoderma cellulase mixture is one of the most commonly used enzymes for cellulosic hydrolysis. During hydrolytic process cellobiose accumulation causes feedback inhibition against most cellobiohydrolases and endoglucanases. In this study, we demonstrated the synergism effects between cellobiose dehydrogenase (CDH) and cellulase both in vitro and in vivo. The CDH from Phanerochaete chrysosporium was heterologously expressed in Pichia pastoris. Supplementation of the purified CDH in Trichoderma cellulase increased the cellulase activities. Especially beta-glucosidase activity was increased by 30-100% varying at different time points. On the other hand, the cdh gene was heterologously expressed in Trichoderma reesei to explore the synergism between CDH and cellulases in vivo. The analyses of gene expression and enzymatic profiles of filter paper activity, carboxymethylcellulase (CMCase) and beta-glucosidase show the increased cellulase activity and the enhanced cellulase production in the cdh-expressing strains. The results elucidate a possible mechanism for diminishing the cellobiose inhibition of cellulase by CDH. These findings provide a novel perspective to make more economic enzyme cocktails for commercial application or explore alternative strategies for generating cellulase-producing strains with higher efficiency.
文章类型	Article
关键词	Tritroderma Reesei Cellulase Cellobiose Inhibition Cellobiose Dehydrogenase Synergy
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.3389/fmicb.2016.00620
关键词[WOS]	HYPOCREA-JECORINA ; BETA-GLUCOSIDASE ; ENZYMATIC-HYDROLYSIS ; GENE ; CELLOBIOHYDROLASE ; DEGRADATION ; EXPRESSION ; INHIBITION ; INDUCTION ; BIOFUELS
收录类别	SCI
语种	英语
WOS研究方向	Microbiology
项目资助者	National Natural Science Foundation of China (NSFC)(31100585) ; Basic Research Project of Qingdao - Qingdao Municipal Science and Technology Commission(12-1-1-9-(1)-jch)
WOS类目	Microbiology
WOS记录号	WOS:000375388100001
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/8297
专题	微生物代谢工程研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Provinc Key Lab Energy Genet, Qingdao, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Provinc Key Lab Synthet Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Wang, Min,Lu, Xuefeng. Exploring the Synergy between Cellobiose Dehydrogenase from Phanerochaete chrysosporium and Cellulase from Trichoderma reesei[J]. FRONTIERS IN MICROBIOLOGY,2016,7.
APA	Wang, Min,&Lu, Xuefeng.(2016).Exploring the Synergy between Cellobiose Dehydrogenase from Phanerochaete chrysosporium and Cellulase from Trichoderma reesei.FRONTIERS IN MICROBIOLOGY,7.
MLA	Wang, Min,et al."Exploring the Synergy between Cellobiose Dehydrogenase from Phanerochaete chrysosporium and Cellulase from Trichoderma reesei".FRONTIERS IN MICROBIOLOGY 7(2016).