Biosynthetic pathway for acrylic acid from glycerol in recombinant Escherichia coli | |
Tong, Wenhua1,2; Xu, Ying1,2; Xian, Mo1; Niu, Wei3; Guo, Jiantao3; Liu, Huizhou1; Zhao, Guang1 | |
2016-06-01 | |
发表期刊 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY |
卷号 | 100期号:11页码:4901-4907 |
摘要 | Acrylic acid is an important industrial feedstock. In this study, a de novo acrylate biosynthetic pathway from inexpensive carbon source glycerol was constructed in Escherichia coli. The acrylic acid was produced from glycerol via 3-hydroxypropionaldehyde, 3-hydroxypropionyl-CoA, and acrylyl-CoA. The acrylate production was improved by screening and site-directed mutagenesis of key enzyme enoyl-CoA hydratase and chromosomal integration of some exogenous genes. Finally, our recombinant strain produced 37.7 mg/L acrylic acid under shaking flask conditions. Although the acrylate production is low, our study shows feasibility of engineering an acrylate biosynthetic pathway from inexpensive carbon source. Furthermore, the reasons for limited acrylate production and further strain optimization that should be performed in the future were also discussed. |
文章类型 | Article |
关键词 | Acrylic Acid Biosynthesis Enoyl-coa Hydratase Recombinant Escherichia Coli Inexpensive Carbon Source |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
DOI | 10.1007/s00253-015-7272-z |
关键词[WOS] | ENOYL-COA HYDRATASE ; 3-HYDROXYPROPIONATE CYCLE ; COENZYME ; ENZYME ; CLONING |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Biotechnology & Applied Microbiology |
项目资助者 | CAS ; CAS(KGZD-EW-606-1-3) ; Taishan Scholars Climbing Program of Shandong(TSPD20150210) |
WOS类目 | Biotechnology & Applied Microbiology |
WOS记录号 | WOS:000376255200015 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qibebt.ac.cn/handle/337004/8283 |
专题 | 生物基材料组群 |
作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Nebraska, Dept Chem, Lincoln, NE 68588 USA |
推荐引用方式 GB/T 7714 | Tong, Wenhua,Xu, Ying,Xian, Mo,et al. Biosynthetic pathway for acrylic acid from glycerol in recombinant Escherichia coli[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2016,100(11):4901-4907. |
APA | Tong, Wenhua.,Xu, Ying.,Xian, Mo.,Niu, Wei.,Guo, Jiantao.,...&Zhao, Guang.(2016).Biosynthetic pathway for acrylic acid from glycerol in recombinant Escherichia coli.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,100(11),4901-4907. |
MLA | Tong, Wenhua,et al."Biosynthetic pathway for acrylic acid from glycerol in recombinant Escherichia coli".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 100.11(2016):4901-4907. |
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