A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo

doi:10.1038/srep26722

QIBEBT-IR > 仿生智能材料研究组

	A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo
	Liu, Ge 1,2,3; Kuang, Shan 1,2; Wu, Shimei4 ; Jin, Weihua 1,2; Sun, Chaomin 1,2
	2016-05-24
发表期刊	SCIENTIFIC REPORTS
卷号	6
摘要	Many polysaccharides isolated from plants have exhibited promising antitumor activities. The aim of this study is to investigate the antitumor activity of the novel polysaccharide named SPS from Sargassum integerrimum, elucidate the underlying anticancer mechanism in a human lung cancer cell line A549, and evaluate its anti-angiogenic activity both in vitro and in vivo. The results show that SPS significantly reduces A549 cells viability in a dose-and time-dependent manner via MTT method. Flow cytometry analysis indicates that SPS could induce cell apoptosis, the loss of mitochondrial membrane potential (MMP), generation of reactive oxygen species (ROS) and G2/M phase cell cycle arrest of A549 cells. Up-regulation of the expressions of P53 and Bax, down-regulation of the expression of Bcl-2, and activation of cleaved caspase-3, caspase-9 and PARP are also detected by western blotting after the treatment of SPS. In addition, SPS inhibits the proliferation, migration and cord formation of human umbilical vein endothelial cells (HUVECs) in vitro, and prevents the vascular development of zebrafish embryos in vivo. Altogether, our data prove the anticancer and anti-angiogenesis properties of SPS, and provide further insights into the potential pharmacological application of SPS as antitumor and antiangiogenic agent against lung cancer.
文章类型	Article
WOS标题词	Science & Technology
DOI	10.1038/srep26722
关键词[WOS]	ENDOTHELIAL-CELLS ; BCL-2 FAMILY ; LUNG-CANCER ; ANTIOXIDANT ACTIVITIES ; INHIBITS ANGIOGENESIS ; MECHANISMS ; ANTITUMOR ; ROS ; ANTIANGIOGENESIS ; PROLIFERATION
收录类别	SCI
语种	英语
WOS研究方向	Science & Technology - Other Topics
项目资助者	Science and Technology Development Foundation of Qingdao Shi'nan District Science and Technology Bureau(2014-14-006-SW) ; Scientific and Technological Innovation Project of Qingdao National Laboratory for Marine Science and Technology(2015ASKJ02) ; Shandong Provincial Natural Science Foundation, China(ZR2014DQ024) ; "100-Talent Project" of Chinese Academy of Sciences
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000376390900001
引用统计	被引频次：48[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/8278
专题	仿生智能材料研究组
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Liu, Ge,Kuang, Shan,Wu, Shimei,et al. A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo[J]. SCIENTIFIC REPORTS,2016,6.
APA	Liu, Ge,Kuang, Shan,Wu, Shimei,Jin, Weihua,&Sun, Chaomin.(2016).A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo.SCIENTIFIC REPORTS,6.
MLA	Liu, Ge,et al."A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo".SCIENTIFIC REPORTS 6(2016).