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Title:
Gold nanoprobe functionalized with specific fusion protein selection from phage display and its application in rapid, selective and sensitive colorimetric biosensing of Staphylococcus aureus
Author: Liu, Pei1,2,3,4; Han, Lei1,2,3,4; Wang, Fei1,2,3,4; Petrenko, Valery A.5; Liu, Aihua1,2,3,4
Source: BIOSENSORS & BIOELECTRONICS
Issued Date: 2016-08-15
Volume: 82, Pages:195-203
Keyword: Specific phage pVIII fusion protein ; Staphylococcus aureus ; Gold nanoparticles ; Colorimetric sensor ; Pathogen detection
DOI: 10.1016/j.bios.2016.03.075
DOC Type: Article
English Abstract: Staphylococcus aureus (S. aureus) is one of the most ubiquitous pathogens in public healthcare worldwide. It holds great insterest in establishing robust analytical method for S. aureus. Herein, we report a S. aureus-specific recognition element, isolated from phage monoclone GQTTLITS, which was selected from f8/8 landscape phage library against S. aureus in a high-throughput way. By fiinctionalizing cysteamine (CS)-stabilized gold nanoparticles (CS-AuNPs) with S. aureus-specific pVIII fusion protein (fusion-pVIII), a bifunctional nanoprobe (CS-AuNPs@fusion-pVIII) for S. aureus was developed. In this strategy, the CS-AuNPs@fusion-pVIII could be induced to aggregate quickly in the presence of target S. aureus, resulting in a rapid colorimetric response of gold nanoparticles. More importantly, the as-designed probe exhibited excellent selectivity over other bacteria. Thus, the CS-AuNPs@fusion-pVIII could be used as the indicator of target S. aureus. This assay can detect as low as 19 CFU mL(-1) S. aureus within 30 min. Further, this approach can be applicable to detect S. aureus in real water samples. Due to its sensitivity, specificity and rapidness, this proposed method is promising for on-site testing of S. aureus without using any costly instruments. (C) 2016 Elsevier B.V. All rights reserved.
WOS Headings: Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
WOS Subject: Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS Subject Extended: Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS Keyword Plus: BIOFUNCTIONAL MAGNETIC NANOPARTICLES ; GRAM-POSITIVE BACTERIA ; BREAST-CANCER CELLS ; LANDSCAPE PHAGE ; SALMONELLA-TYPHIMURIUM ; PATHOGENIC BACTERIA ; PEPTIDE ; LIBRARY ; SURFACE ; DELIVERY
Indexed Type: SCI
Funder: National Natural Science Foundation of China(21275152 ; 21475144)
Language: 英语
WOS ID: WOS:000376545400029
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Content Type: 期刊论文
URI: http://ir.qibebt.ac.cn/handle/337004/8274
Appears in Collections:生物传感技术团队_期刊论文

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description.institution: 1.Chinese Acad Sci, Shandong Prov Key Lab Synthet Biol, Lab Biosensing, Qingdao Inst Bioenergy & Bioproc Technol, 189 Songling Rd, Qingdao 266101, Peoples R China
2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
3.Qingdao Univ, Inst Biosensing, Qingdao 266071, Peoples R China
4.Qingdao Univ, Coll Chem Sci & Engn, Qingdao 266071, Peoples R China
5.Auburn Univ, Dept Pathobiol, 269 Greene Hall, Auburn, AL 36849 USA

Recommended Citation:
Liu, Pei,Han, Lei,Wang, Fei,et al. Gold nanoprobe functionalized with specific fusion protein selection from phage display and its application in rapid, selective and sensitive colorimetric biosensing of Staphylococcus aureus[J]. BIOSENSORS & BIOELECTRONICS,2016,82:195-203.
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