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Gold nanoprobe functionalized with specific fusion protein selection from phage display and its application in rapid, selective and sensitive colorimetric biosensing of Staphylococcus aureus
Liu, Pei1,2,3,4; Han, Lei1,2,3,4; Wang, Fei1,2,3,4; Petrenko, Valery A.5; Liu, Aihua1,2,3,4
2016-08-15
发表期刊BIOSENSORS & BIOELECTRONICS
卷号82页码:195-203
摘要Staphylococcus aureus (S. aureus) is one of the most ubiquitous pathogens in public healthcare worldwide. It holds great insterest in establishing robust analytical method for S. aureus. Herein, we report a S. aureus-specific recognition element, isolated from phage monoclone GQTTLITS, which was selected from f8/8 landscape phage library against S. aureus in a high-throughput way. By fiinctionalizing cysteamine (CS)-stabilized gold nanoparticles (CS-AuNPs) with S. aureus-specific pVIII fusion protein (fusion-pVIII), a bifunctional nanoprobe (CS-AuNPs@fusion-pVIII) for S. aureus was developed. In this strategy, the CS-AuNPs@fusion-pVIII could be induced to aggregate quickly in the presence of target S. aureus, resulting in a rapid colorimetric response of gold nanoparticles. More importantly, the as-designed probe exhibited excellent selectivity over other bacteria. Thus, the CS-AuNPs@fusion-pVIII could be used as the indicator of target S. aureus. This assay can detect as low as 19 CFU mL(-1) S. aureus within 30 min. Further, this approach can be applicable to detect S. aureus in real water samples. Due to its sensitivity, specificity and rapidness, this proposed method is promising for on-site testing of S. aureus without using any costly instruments. (C) 2016 Elsevier B.V. All rights reserved.
文章类型Article
关键词Specific Phage Pviii Fusion Protein Staphylococcus Aureus Gold Nanoparticles Colorimetric Sensor Pathogen Detection
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.bios.2016.03.075
关键词[WOS]BIOFUNCTIONAL MAGNETIC NANOPARTICLES ; GRAM-POSITIVE BACTERIA ; BREAST-CANCER CELLS ; LANDSCAPE PHAGE ; SALMONELLA-TYPHIMURIUM ; PATHOGENIC BACTERIA ; PEPTIDE ; LIBRARY ; SURFACE ; DELIVERY
收录类别SCI
语种英语
WOS研究方向Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
项目资助者National Natural Science Foundation of China(21275152 ; 21475144)
WOS类目Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS记录号WOS:000376545400029
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/8274
专题生物传感技术团队
作者单位1.Chinese Acad Sci, Shandong Prov Key Lab Synthet Biol, Lab Biosensing, Qingdao Inst Bioenergy & Bioproc Technol, 189 Songling Rd, Qingdao 266101, Peoples R China
2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
3.Qingdao Univ, Inst Biosensing, Qingdao 266071, Peoples R China
4.Qingdao Univ, Coll Chem Sci & Engn, Qingdao 266071, Peoples R China
5.Auburn Univ, Dept Pathobiol, 269 Greene Hall, Auburn, AL 36849 USA
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Liu, Pei,Han, Lei,Wang, Fei,et al. Gold nanoprobe functionalized with specific fusion protein selection from phage display and its application in rapid, selective and sensitive colorimetric biosensing of Staphylococcus aureus[J]. BIOSENSORS & BIOELECTRONICS,2016,82:195-203.
APA Liu, Pei,Han, Lei,Wang, Fei,Petrenko, Valery A.,&Liu, Aihua.(2016).Gold nanoprobe functionalized with specific fusion protein selection from phage display and its application in rapid, selective and sensitive colorimetric biosensing of Staphylococcus aureus.BIOSENSORS & BIOELECTRONICS,82,195-203.
MLA Liu, Pei,et al."Gold nanoprobe functionalized with specific fusion protein selection from phage display and its application in rapid, selective and sensitive colorimetric biosensing of Staphylococcus aureus".BIOSENSORS & BIOELECTRONICS 82(2016):195-203.
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