| Synergistic effect between the recombinant exo-inulinase and endo-inulinase on inulin hydrolysis | |
| Liu, Yuan1; Zhou, Shun-Hua1; Cheng, Yu-Rong2; Chi, Zhe1; Chi, Zhen-Ming1; Liu, Guang-Lei1 | |
| 2016-06-01 | |
| 发表期刊 | JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
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| 卷号 | 128页码:27-38 |
| 摘要 | An exo-inulinase gene from Kluyveromyces marxianus and an endo-inulinase gene from Aspergillus niger were cloned and expressed in Yarrowia lipolytica, respectively. Then, the purified recombinant exo-inulinase (rEXINU) and endo-inulinase (rENINU), either individual or combination with another, were used for hydrolysis of inulin and inulin in tuber meal of Jerusalem artichoke. After the analysis of the dynamic hydrolysis process, it was found that the rEXINU and the rENINU acted synergistically with each other, and the maximum degree of synergistic effect on the level of fructose formation (DSEFru) could be obtained with a molar ratio of the rEXINU to the rENINU at 1:1. Furthermore, the investigation of the hydrolysis product in the synergistic hydrolysis process showed that a wide range of oligosaccharides were first generated and then converted to fructose. Based on the enzyme kinetics, substrate specificity and product inhibition of the rEXINU and the rENINU, a synergism mechanism of the them for the inulin hydrolysis was proposed. During the process of the synergistic action, the rENINU preferentially degraded the inulin with high DP, but was inhibited by the produced oligosaccharides. Then the released oligosaccharides which were the optimum substrates of the rEXIUN were rapidly hydrolyzed by the enzyme to produce fructose and glucose. Meanwhile, this process relieved the inhibition of the produced oligosaccharides to the rENINU. Besides, in the synergistic hydrolysis of the inulin in tuber meal of Jerusalem artichoke, the depolymerization of the rENINU could result in the increase of the subtrate solubility to improve the hydrolysis efficiency. Therefore, this kind of the synergistic effect between the exo-inulinase and the endo-inulinase may provide a potential driving force in the industrial application of inulin and inulin-containing materials. (C) 2016 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 关键词 | Synergistic Effect Exo-inulinase Endo-inulinase Inulin Inulo-oligosaccharides |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| DOI | 10.1016/j.molcatb.2016.03.005 |
| 关键词[WOS] | HETEROLOGOUS PROTEIN EXPRESSION ; SACCHAROMYCES-CEREVISIAE ; KLUYVEROMYCES-MARXIANUS ; ENHANCED EXPRESSION ; YARROWIA-LIPOLYTICA ; ASPERGILLUS-NIGER ; ALPHA-AMYLASE ; HYDROLASE ; GENE ; GUILLIERMONDII |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 项目资助者 | National Natural Science Foundation of China(31400047) ; General Financial Grant from the China Postdoctoral Science Foundation(2014M560576) |
| WOS类目 | Biochemistry & Molecular Biology ; Chemistry, Physical |
| WOS记录号 | WOS:000375008100004 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/8268 |
| 专题 | 代谢物组学研究组 |
| 作者单位 | 1.Ocean Univ China, Yushan Rd 5, Qingdao 266003, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Songling Rd 189, Qingdao 266101, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yuan,Zhou, Shun-Hua,Cheng, Yu-Rong,et al. Synergistic effect between the recombinant exo-inulinase and endo-inulinase on inulin hydrolysis[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2016,128:27-38. |
| APA | Liu, Yuan,Zhou, Shun-Hua,Cheng, Yu-Rong,Chi, Zhe,Chi, Zhen-Ming,&Liu, Guang-Lei.(2016).Synergistic effect between the recombinant exo-inulinase and endo-inulinase on inulin hydrolysis.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,128,27-38. |
| MLA | Liu, Yuan,et al."Synergistic effect between the recombinant exo-inulinase and endo-inulinase on inulin hydrolysis".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 128(2016):27-38. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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