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Synergistic effect between the recombinant exo-inulinase and endo-inulinase on inulin hydrolysis
Liu, Yuan1; Zhou, Shun-Hua1; Cheng, Yu-Rong2; Chi, Zhe1; Chi, Zhen-Ming1; Liu, Guang-Lei1
2016-06-01
发表期刊JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
卷号128页码:27-38
摘要An exo-inulinase gene from Kluyveromyces marxianus and an endo-inulinase gene from Aspergillus niger were cloned and expressed in Yarrowia lipolytica, respectively. Then, the purified recombinant exo-inulinase (rEXINU) and endo-inulinase (rENINU), either individual or combination with another, were used for hydrolysis of inulin and inulin in tuber meal of Jerusalem artichoke. After the analysis of the dynamic hydrolysis process, it was found that the rEXINU and the rENINU acted synergistically with each other, and the maximum degree of synergistic effect on the level of fructose formation (DSEFru) could be obtained with a molar ratio of the rEXINU to the rENINU at 1:1. Furthermore, the investigation of the hydrolysis product in the synergistic hydrolysis process showed that a wide range of oligosaccharides were first generated and then converted to fructose. Based on the enzyme kinetics, substrate specificity and product inhibition of the rEXINU and the rENINU, a synergism mechanism of the them for the inulin hydrolysis was proposed. During the process of the synergistic action, the rENINU preferentially degraded the inulin with high DP, but was inhibited by the produced oligosaccharides. Then the released oligosaccharides which were the optimum substrates of the rEXIUN were rapidly hydrolyzed by the enzyme to produce fructose and glucose. Meanwhile, this process relieved the inhibition of the produced oligosaccharides to the rENINU. Besides, in the synergistic hydrolysis of the inulin in tuber meal of Jerusalem artichoke, the depolymerization of the rENINU could result in the increase of the subtrate solubility to improve the hydrolysis efficiency. Therefore, this kind of the synergistic effect between the exo-inulinase and the endo-inulinase may provide a potential driving force in the industrial application of inulin and inulin-containing materials. (C) 2016 Elsevier B.V. All rights reserved.
文章类型Article
关键词Synergistic Effect Exo-inulinase Endo-inulinase Inulin Inulo-oligosaccharides
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1016/j.molcatb.2016.03.005
关键词[WOS]HETEROLOGOUS PROTEIN EXPRESSION ; SACCHAROMYCES-CEREVISIAE ; KLUYVEROMYCES-MARXIANUS ; ENHANCED EXPRESSION ; YARROWIA-LIPOLYTICA ; ASPERGILLUS-NIGER ; ALPHA-AMYLASE ; HYDROLASE ; GENE ; GUILLIERMONDII
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
项目资助者National Natural Science Foundation of China(31400047) ; General Financial Grant from the China Postdoctoral Science Foundation(2014M560576)
WOS类目Biochemistry & Molecular Biology ; Chemistry, Physical
WOS记录号WOS:000375008100004
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/8268
专题代谢物组学研究组
作者单位1.Ocean Univ China, Yushan Rd 5, Qingdao 266003, Shandong, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Songling Rd 189, Qingdao 266101, Shandong, Peoples R China
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GB/T 7714
Liu, Yuan,Zhou, Shun-Hua,Cheng, Yu-Rong,et al. Synergistic effect between the recombinant exo-inulinase and endo-inulinase on inulin hydrolysis[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2016,128:27-38.
APA Liu, Yuan,Zhou, Shun-Hua,Cheng, Yu-Rong,Chi, Zhe,Chi, Zhen-Ming,&Liu, Guang-Lei.(2016).Synergistic effect between the recombinant exo-inulinase and endo-inulinase on inulin hydrolysis.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,128,27-38.
MLA Liu, Yuan,et al."Synergistic effect between the recombinant exo-inulinase and endo-inulinase on inulin hydrolysis".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 128(2016):27-38.
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