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Title:
Improving the activity of Trichoderma reesei cel7B through stabilizing the transition state
Author: Wang, Yefei1,2; Song, Xiangfei1,2; Zhang, Shujun1,2; Li, Jingwen1,2; Shu, Zhiyu1,2; He, Chunyan1,2; Huang, Qingshan1,2; Yao, Lishan1,2
Source: BIOTECHNOLOGY AND BIOENGINEERING
Issued Date: 2016-06-01
Volume: 113, Issue:6, Pages:1171-1177
Keyword: cel7B ; transition state ; cellulase ; molecular dynamics simulation ; protein engineering
DOI: 10.1002/bit.25887
DOC Type: Article
English Abstract: Trichoderma reesei (Tr.) cellulases, which convert cellulose to reducing sugars, are a promising catalyst used in the lignocellulosic biofuel production. Improving Tr. cellulases activity, though very difficult, is highly desired due to the recalcitrance of lignocellulose. Meanwhile, it is preferable to enhance the cellulase's promiscuity so that substrates other than cellulose can also be hydrolyzed. In this work, an attempt is made to improve the catalytic activity of a major endogluanase Tr. Cel7B against xylan which crosslinks with cellulose in lignocellulose. By using quantum mechanics/molecular mechanics (QM/MM) molecular dynamics (MD) simulations, the transition state of the xylo-oligosaccharide hydrolysis is identified. Then, mutations are introduced and their effect on the transition state stabilization is ranked based on the free energy calculations. Seven top ranked mutants are evaluated experimentally. Three mutants A208Q, A222D, and G230R show a higher activity than the wild-type Tr. Cel7B in the hydrolysis of xylan (by up to 47%) as well as filter paper (by up to 50%). The combination of the single mutants can further improve the enzyme activity. Our work demonstrates that the free energy method is effective in engineering the Tr. Cel7B activity against xylan and cellulose, and thus may also be useful for improving the activity of other Tr. cellulases. (C) 2015 Wiley Periodicals, Inc.
WOS Headings: Science & Technology ; Life Sciences & Biomedicine
WOS Subject: Biotechnology & Applied Microbiology
WOS Subject Extended: Biotechnology & Applied Microbiology
WOS Keyword Plus: CELLULOSE HYDROLYSIS ; CELLOBIOHYDROLASE I ; QM/MM METADYNAMICS ; CATALYTIC MECHANISM ; CRYSTAL-STRUCTURE ; ENDOGLUCANASE-I ; FREE-ENERGIES ; ENZYMES ; RESOLUTION ; COMPLEX
Indexed Type: SCI
Funder: National Natural Science Foundation of China(21173247 ; "135'' Projects Fund of CAS-QIBEBT Director Innovation Foundation ; 21106169 ; 31400061)
Language: 英语
WOS ID: WOS:000375119900003
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Content Type: 期刊论文
URI: http://ir.qibebt.ac.cn/handle/337004/8266
Appears in Collections:仿真与模拟团队_期刊论文

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description.institution: 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, Qingdao 266061, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biofuels, Qingdao 266061, Peoples R China

Recommended Citation:
Wang, Yefei,Song, Xiangfei,Zhang, Shujun,et al. Improving the activity of Trichoderma reesei cel7B through stabilizing the transition state[J]. BIOTECHNOLOGY AND BIOENGINEERING,2016,113(6):1171-1177.
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