| Development of a 3-hydroxypropionate resistant Escherichia coli strain | |
| Liu, Min1,2; Han, Xueping1,2; Xian, Mo1; Ding, Yamei3; Liu, Huizhou1; Zhao, Guang1 | |
| 2016 | |
| 发表期刊 | BIOENGINEERED
 |
| 卷号 | 7期号:1页码:21-27 |
| 摘要 | 3-hydroxypropionate (3HP) is an important platform chemical, and its biosynthesis is severely restricted by the toxicity of 3HP on cell growth and survival. To improve Escherichia coli resistance to 3HP and reduce the total production cost in industrial applications, we have identified variations in protein expression level exposed to sub-lethal concentration of this chemical using 2-dimensional gel electrophoresis. Under 3HP stress, the amount of 46 proteins was increased while the amount of 23 proteins was reduced. According to the proteomic results, overexpression of some identified proteins significantly increased the E. coli survival rate under 3HP stress. This study shed light on clues for developing E. coli strains with higher resistance to 3HP toxicity and lower production cost for industrial applications. |
| 文章类型 | Article |
| 关键词 | Bacterial Stress Response Comparative Proteomics Escherichia Coli 3-hydroxypropionate 3-hydroxypropionate Resistance |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1080/21655979.2015.1122143 |
| 关键词[WOS] | PROLINE BIOSYNTHESIS ; PROTEOME ANALYSIS ; ACID RESISTANCE ; STRESS ; IDENTIFICATION ; DEHYDROGENASE ; MECHANISMS ; TOLERANCE ; SURVIVAL ; GLYCEROL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 项目资助者 | National Natural Science Foundation of China(31200030) ; "135" Projects Fund of CAS-QIBEBT Director Innovation Foundation ; 100-Talent Project of CAS ; Taishan Scholar Climbing Program of Shandong Province(TSPD20150210) ; CAS(KGZD-EW-606-1-3) |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000373159100009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/8246 |
| 专题 | 生物基材料组群 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Min,Han, Xueping,Xian, Mo,et al. Development of a 3-hydroxypropionate resistant Escherichia coli strain[J]. BIOENGINEERED,2016,7(1):21-27. |
| APA | Liu, Min,Han, Xueping,Xian, Mo,Ding, Yamei,Liu, Huizhou,&Zhao, Guang.(2016).Development of a 3-hydroxypropionate resistant Escherichia coli strain.BIOENGINEERED,7(1),21-27. |
| MLA | Liu, Min,et al."Development of a 3-hydroxypropionate resistant Escherichia coli strain".BIOENGINEERED 7.1(2016):21-27. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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