New insights into the structural basis of DNA recognition by HINa and HINb domains of IFI16

doi:10.1093/jmcb/mjv053

QIBEBT-IR > 代谢物组学研究组

	New insights into the structural basis of DNA recognition by HINa and HINb domains of IFI16
	Ni, Xiangmin 1,2; Ru, Heng 2; Ma, Feng 3; Zhao, Lixia 2; Shaw, Neil 2; Feng, Yingang4,5 ; Ding, Wei 2; Gong, Weibin 2; Wang, Qiaofeng 1; Ouyang, Songying 2; Cheng, Genhong 3; Liu, Zhi-Jie 1,2,6
	2016-02-01
发表期刊	JOURNAL OF MOLECULAR CELL BIOLOGY
卷号	8 期号:1 页码:51-61
摘要	Interferon gamma-inducible protein 16 (IFI16) senses DNA in the cytoplasm and the nucleus by using two tandem hematopoietic interferon-inducible nuclear (HIN) domains, HINa and HINb, through the cooperative assembly of IFI16 filaments on double-stranded DNA (dsDNA). The role of HINa in sensing DNA is not clearly understood. Here, we describe the crystal structure of the HINa domain in complex with DNA at 2.55 resolution and provide the first insight into the mode of DNA binding by the HINa domain. The structure reveals the presence of two oligosaccharide/nucleotide-binding (OB) folds with a unique DNA-binding surface. HINa uses loop L-45 of the canonical OB2 fold to bind to the DNA backbone. The dsDNA is recognized as two single strands of DNA. Interestingly, deletion of HINb compromises the ability of IFI16 to induce IFN-beta, while HINa mutants impaired in DNA binding enhance the production of IFN-beta. These results shed light on the roles of IFI16 HIN domains in DNA recognition and innate immune responses.
文章类型	Article
关键词	Interferon Gamma-inducible Protein 16 (Ifi16) Hematopoietic Interferon-inducible Nuclear (Hin) Domain Dna Recognition Innate Immune Responses
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1093/jmcb/mjv053
关键词[WOS]	CYCLIC GMP-AMP ; INNATE IMMUNE-RESPONSE ; TOLL-LIKE RECEPTORS ; DENDRITIC CELLS ; INTRACELLULAR DNA ; CYTOSOLIC DNA ; PROVIDES INSIGHTS ; CYTOPLASMIC DNA ; I INTERFERON ; PROTEIN
收录类别	SCI
语种	英语
WOS研究方向	Cell Biology
项目资助者	National Natural Science Foundation of China(31570875 ; Ministry of Science and Technology of China(2014CB910400 ; National Science and Technology Major Project of China(2013ZX10004-602) ; Beijing Nova Program(Z141102001814020) ; Youth Innovation Promotion Association CAS ; special project of Ebola virus research from president foundation of Chinese Academy of Sciences ; 31330019 ; 2013CB911103) ; 31200559 ; 81590761)
WOS类目	Cell Biology
WOS记录号	WOS:000372615600006
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/8065
专题	代谢物组学研究组
作者单位	1.Kunming Med Univ, Inst Mol & Clin Med, Kunming 650500, Peoples R China 2.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 3.Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Lab Single Cell Oil, Qingdao 266101, Peoples R China 5.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China 6.ShanghaiTech Univ, IHuman Inst, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Ni, Xiangmin,Ru, Heng,Ma, Feng,et al. New insights into the structural basis of DNA recognition by HINa and HINb domains of IFI16[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2016,8(1):51-61.
APA	Ni, Xiangmin.,Ru, Heng.,Ma, Feng.,Zhao, Lixia.,Shaw, Neil.,...&Liu, Zhi-Jie.(2016).New insights into the structural basis of DNA recognition by HINa and HINb domains of IFI16.JOURNAL OF MOLECULAR CELL BIOLOGY,8(1),51-61.
MLA	Ni, Xiangmin,et al."New insights into the structural basis of DNA recognition by HINa and HINb domains of IFI16".JOURNAL OF MOLECULAR CELL BIOLOGY 8.1(2016):51-61.