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Integration of bacterial expansin-like proteins into cellulosome promotes the cellulose degradation
Chen, Chao1,2,3,4; Cui, Zhenling1,2,3,4; Song, Xiangfei2,3; Liu, Ya-Jun1,2,3; Cui, Qiu1,2,3; Feng, Yingang1,2,3
2016-03-01
发表期刊APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷号100期号:5页码:2203-2212
摘要

Cellulosomes are multi-enzyme complexes assembled by cellulases and hemicellulases through dockerin-cohesin interactions, which are the most efficient system for the degradation of lignocellulosic resources in nature. Recent genomic analysis of a cellulosome-producing anaerobe Clostridium clariflavum DSM 19732 revealed that two expansin-like proteins, Clocl_1298 and Clocl_1862, contain a dockerin module, which suggests that they are components of the cellulosome. Bacterial expansin-like proteins do not have hydrolytic activities, but can facilitate the degradation of cellulosic biomass via synergistic effects with cellulases. In this study, the synergistic effect of the expansin-like proteins with both native and designer cellulosomes was investigated. The free expansin-like proteins, including expansin-like domains of Clocl_1298 and Clocl_1862, as well as a well-studied bacterial expansin-like protein BsEXLX1 from Bacillus subtilis, promoted the cellulose degradation by native cellulosomes, indicating the cellulosomal expansin-like proteins have the synergistic function. When they were integrated into a trivalent designer cellulosome, the synergistic effect was further amplified. The sequence and structure analyses indicated that these cellulosomal expansin-like proteins share the conserved functional mechanism with other bacterial expansin-like proteins. These results indicated that non-catalytic expansin-like proteins in the cellulosome can enhance the activity of the cellulosome in lignocellulose degradation. The involvement of functional expansin-like proteins in the cellulosome also implies new physiological functions of bacterial expansin-like proteins and cellulosomes.

文章类型Article
关键词Expansin Cellulosome Cellulose Hydrolysis Dockerin Clostridium Clariflavum
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s00253-015-7071-6
关键词[WOS]CLOSTRIDIUM-THERMOCELLUM CELLULOSOME ; BACILLUS-SUBTILIS ; BINDING CHARACTERISTICS ; GENE-EXPRESSION ; CELLULOLYTICUM ; HYDROLYSIS ; IDENTIFICATION ; CLARIFLAVUM ; COMPONENTS ; INHIBITOR
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology
项目资助者National Basic Research Program of China (973 Program)(2011CB707404) ; National Natural Science Foundation of China(31270784 ; 31300035 ; 31470210)
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000371243500016
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/8052
专题代谢物组学研究组
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Lab Single Cell Oil, 189 Songling Rd, Qingdao 266101, Shandong, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, 189 Songling Rd, Qingdao 266101, Shandong, Peoples R China
3.Chinese Acad Sci, CAS Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, 189 Songling Rd, Qingdao 266101, Shandong, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Chen, Chao,Cui, Zhenling,Song, Xiangfei,et al. Integration of bacterial expansin-like proteins into cellulosome promotes the cellulose degradation[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2016,100(5):2203-2212.
APA Chen, Chao,Cui, Zhenling,Song, Xiangfei,Liu, Ya-Jun,Cui, Qiu,&Feng, Yingang.(2016).Integration of bacterial expansin-like proteins into cellulosome promotes the cellulose degradation.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,100(5),2203-2212.
MLA Chen, Chao,et al."Integration of bacterial expansin-like proteins into cellulosome promotes the cellulose degradation".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 100.5(2016):2203-2212.
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