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Title:
A Label-Free Electrochemical Impedance Cytosensor Based on Specific Peptide-Fused Phage Selected from Landscape Phage Library
Author: Han, Lei1,2,3; Liu, Pei3; Petrenko, Valery A.4; Liu, Aihua1,2,3
Source: SCIENTIFIC REPORTS
Issued Date: 2016-02-24
Volume: 6
DOI: 10.1038/srep22199
DOC Type: Article
English Abstract: One of the major challenges in the design of biosensors for cancer diagnosis is to introduce a low-cost and selective probe that can recognize cancer cells. In this paper, we combined the phage display technology and electrochemical impedance spectroscopy (EIS) to develop a label-free cytosensor for the detection of cancer cells, without complicated purification of recognition elements. Fabrication steps of the cytosensing interface were monitored by EIS. Due to the high specificity of the displayed octapeptides and avidity effect of their multicopy display on the phage scaffold, good biocompatibility of recombinant phage, the fibrous nanostructure of phage, and the inherent merits of EIS technology, the proposed cytosensor demonstrated a wide linear range (2.0 x 10(2) - 2.0 x 10(8) cells mL(-1)), a low limit of detection (79 cells mL(-1), S/N = 3), high specificity, good inter-and intra-assay reproducibility and satisfactory storage stability. This novel cytosensor designing strategy will open a new prospect for rapid and label-free electrochemical platform for tumor diagnosis.
WOS Headings: Science & Technology
WOS Subject: Multidisciplinary Sciences
WOS Subject Extended: Science & Technology - Other Topics
WOS Keyword Plus: SURFACE-PLASMON RESONANCE ; COVALENT VIRUS LAYER ; IMPEDIMETRIC SENSOR ; CANCER-CELLS ; LUNG-CANCER ; SPECTROSCOPY ; DISPLAY ; BACTERIA ; APTAMER ; CARBOHYDRATE
Indexed Type: SCI
Funder: National Natural Science Foundation of China(91227116 ; National Institute of Health US grant(1U54CA151881-0) ; 21275152 ; 21475144)
Language: 英语
WOS ID: WOS:000370773500001
Citation statistics:
Content Type: 期刊论文
URI: http://ir.qibebt.ac.cn/handle/337004/8018
Appears in Collections:生物传感技术团队_期刊论文

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description.institution: 1.Qingdao Univ, Inst Biosensing & Invitro Diagnost, 38 Dengzhou Rd, Qingdao 266021, Peoples R China
2.Qingdao Univ, Coll Med, 38 Dengzhou Rd, Qingdao 266021, Peoples R China
3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biosensing, 189 Songling Rd, Qingdao 266101, Peoples R China
4.Auburn Univ, Coll Vet Med, Dept Pathobiol, 269 Greene Hall, Auburn, AL 36849 USA

Recommended Citation:
Han, Lei,Liu, Pei,Petrenko, Valery A.,et al. A Label-Free Electrochemical Impedance Cytosensor Based on Specific Peptide-Fused Phage Selected from Landscape Phage Library[J]. SCIENTIFIC REPORTS,2016,6.
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