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A Label-Free Electrochemical Impedance Cytosensor Based on Specific Peptide-Fused Phage Selected from Landscape Phage Library
Han, Lei1,2,3; Liu, Pei3; Petrenko, Valery A.4; Liu, Aihua1,2,3
2016-02-24
发表期刊SCIENTIFIC REPORTS
卷号6
摘要One of the major challenges in the design of biosensors for cancer diagnosis is to introduce a low-cost and selective probe that can recognize cancer cells. In this paper, we combined the phage display technology and electrochemical impedance spectroscopy (EIS) to develop a label-free cytosensor for the detection of cancer cells, without complicated purification of recognition elements. Fabrication steps of the cytosensing interface were monitored by EIS. Due to the high specificity of the displayed octapeptides and avidity effect of their multicopy display on the phage scaffold, good biocompatibility of recombinant phage, the fibrous nanostructure of phage, and the inherent merits of EIS technology, the proposed cytosensor demonstrated a wide linear range (2.0 x 10(2) - 2.0 x 10(8) cells mL(-1)), a low limit of detection (79 cells mL(-1), S/N = 3), high specificity, good inter-and intra-assay reproducibility and satisfactory storage stability. This novel cytosensor designing strategy will open a new prospect for rapid and label-free electrochemical platform for tumor diagnosis.
文章类型Article
WOS标题词Science & Technology
DOI10.1038/srep22199
关键词[WOS]SURFACE-PLASMON RESONANCE ; COVALENT VIRUS LAYER ; IMPEDIMETRIC SENSOR ; CANCER-CELLS ; LUNG-CANCER ; SPECTROSCOPY ; DISPLAY ; BACTERIA ; APTAMER ; CARBOHYDRATE
收录类别SCI
语种英语
WOS研究方向Science & Technology - Other Topics
项目资助者National Natural Science Foundation of China(91227116 ; National Institute of Health US grant(1U54CA151881-0) ; 21275152 ; 21475144)
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000370773500001
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/8018
专题生物传感技术团队
作者单位1.Qingdao Univ, Inst Biosensing & Invitro Diagnost, 38 Dengzhou Rd, Qingdao 266021, Peoples R China
2.Qingdao Univ, Coll Med, 38 Dengzhou Rd, Qingdao 266021, Peoples R China
3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biosensing, 189 Songling Rd, Qingdao 266101, Peoples R China
4.Auburn Univ, Coll Vet Med, Dept Pathobiol, 269 Greene Hall, Auburn, AL 36849 USA
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Han, Lei,Liu, Pei,Petrenko, Valery A.,et al. A Label-Free Electrochemical Impedance Cytosensor Based on Specific Peptide-Fused Phage Selected from Landscape Phage Library[J]. SCIENTIFIC REPORTS,2016,6.
APA Han, Lei,Liu, Pei,Petrenko, Valery A.,&Liu, Aihua.(2016).A Label-Free Electrochemical Impedance Cytosensor Based on Specific Peptide-Fused Phage Selected from Landscape Phage Library.SCIENTIFIC REPORTS,6.
MLA Han, Lei,et al."A Label-Free Electrochemical Impedance Cytosensor Based on Specific Peptide-Fused Phage Selected from Landscape Phage Library".SCIENTIFIC REPORTS 6(2016).
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