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Title:
Rational design of xylose dehydrogenase for improved thermostability and its application in development of efficient enzymatic biofuel cell
Author: Feng, Ruirui1,2,3,4; Liang, Bo3,4,5; Hou, Chuantao3,4; Han, Dongfei3,4; Han, Lei3,4,5; Lang, Qiaolin3,4; Liu, Aihua3,4,5; Han, Lihui1,2
Source: ENZYME AND MICROBIAL TECHNOLOGY
Issued Date: 2016-03-01
Volume: 84, Pages:78-85
Keyword: Homology modeling ; Xylose dehydrogenase mutants ; Thermostability ; Microbial surface display ; Xylose/O-2 based biofuel cell
DOI: 10.1016/j.enzmictec.2015.12.002
DOC Type: Article
English Abstract: In this paper, the construction of 3D model structure of xylose dehydrogenase (XDH) by using homology modeling to guide the rational design of the enzyme for improving thermostability was reported. Three XDH mutants of NA-1 (+249L), NA-2 (G149P) and NA-3 (+249L/G149P) were designed and displayed on the surface of bacteria. Among them, bacteria displaying NA-1 (NA-1-bacteria) exhibited superior thermostability without compromising its activity and substrate specificity in comparison with its wild-type counterpart. NA-1-bacteria retained its original activity after incubation at room temperature for one month with the half-life of 9.8 days at 40 degrees C. Finally, the NA-1-bacteria were applied to construct xylose/O-2 based biofuel cell with good performance including enhanced operational stability. Thus, the approach described here could be explored for engineering of other enzymes for improving certain characters without three-dimensional structure identified by experimental methods. (C) 2015 Elsevier Inc. All rights reserved.
WOS Headings: Science & Technology ; Life Sciences & Biomedicine
WOS Subject: Biotechnology & Applied Microbiology
WOS Subject Extended: Biotechnology & Applied Microbiology
WOS Keyword Plus: GLUCOSE-DEHYDROGENASE ; SURFACE DISPLAY ; WHOLE-CELL ; MODIFIED-ELECTRODE ; BIOSENSOR ; PROTEIN ; BACTERIA ; COIMMOBILIZATION ; METABOLISM ; SIMULATION
Indexed Type: SCI
Funder: National Natural Science Foundation of China(91227116 ; Qingdao Institute of Bioenergy and Bioprocess Technology Director Innovation Foundation for Young Scientists(Y37203210S) ; 31200598 ; 21275152 ; 31300663 ; 21475144 ; 31500799)
Language: 英语
WOS ID: WOS:000370306400010
Citation statistics:
Content Type: 期刊论文
URI: http://ir.qibebt.ac.cn/handle/337004/8015
Appears in Collections:生物传感技术团队_期刊论文

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description.institution: 1.Ocean Univ China, Minist Educ, Key Lab Marine Chem Theory & Technol, 238 Songling Rd, Qingdao 266100, Peoples R China
2.Ocean Univ China, Coll Chem & Chem Engn, 238 Songling Rd, Qingdao 266100, Peoples R China
3.Chinese Acad Sci, Lab Biosensing, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, 189 Songling Rd, Qingdao 266101, Peoples R China
4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, 189 Songling Rd, Qingdao 266101, Peoples R China
5.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China

Recommended Citation:
Feng, Ruirui,Liang, Bo,Hou, Chuantao,et al. Rational design of xylose dehydrogenase for improved thermostability and its application in development of efficient enzymatic biofuel cell[J]. ENZYME AND MICROBIAL TECHNOLOGY,2016,84:78-85.
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