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Rational design of xylose dehydrogenase for improved thermostability and its application in development of efficient enzymatic biofuel cell
Feng, Ruirui1,2,3,4; Liang, Bo3,4,5; Hou, Chuantao3,4; Han, Dongfei3,4; Han, Lei3,4,5; Lang, Qiaolin3,4; Liu, Aihua3,4,5; Han, Lihui1,2
2016-03-01
发表期刊ENZYME AND MICROBIAL TECHNOLOGY
卷号84页码:78-85
摘要In this paper, the construction of 3D model structure of xylose dehydrogenase (XDH) by using homology modeling to guide the rational design of the enzyme for improving thermostability was reported. Three XDH mutants of NA-1 (+249L), NA-2 (G149P) and NA-3 (+249L/G149P) were designed and displayed on the surface of bacteria. Among them, bacteria displaying NA-1 (NA-1-bacteria) exhibited superior thermostability without compromising its activity and substrate specificity in comparison with its wild-type counterpart. NA-1-bacteria retained its original activity after incubation at room temperature for one month with the half-life of 9.8 days at 40 degrees C. Finally, the NA-1-bacteria were applied to construct xylose/O-2 based biofuel cell with good performance including enhanced operational stability. Thus, the approach described here could be explored for engineering of other enzymes for improving certain characters without three-dimensional structure identified by experimental methods. (C) 2015 Elsevier Inc. All rights reserved.
文章类型Article
关键词Homology Modeling Xylose Dehydrogenase Mutants Thermostability Microbial Surface Display Xylose/o-2 Based Biofuel Cell
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.enzmictec.2015.12.002
关键词[WOS]GLUCOSE-DEHYDROGENASE ; SURFACE DISPLAY ; WHOLE-CELL ; MODIFIED-ELECTRODE ; BIOSENSOR ; PROTEIN ; BACTERIA ; COIMMOBILIZATION ; METABOLISM ; SIMULATION
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology
项目资助者National Natural Science Foundation of China(91227116 ; Qingdao Institute of Bioenergy and Bioprocess Technology Director Innovation Foundation for Young Scientists(Y37203210S) ; 31200598 ; 21275152 ; 31300663 ; 21475144 ; 31500799)
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000370306400010
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/8015
专题生物传感技术团队
作者单位1.Ocean Univ China, Minist Educ, Key Lab Marine Chem Theory & Technol, 238 Songling Rd, Qingdao 266100, Peoples R China
2.Ocean Univ China, Coll Chem & Chem Engn, 238 Songling Rd, Qingdao 266100, Peoples R China
3.Chinese Acad Sci, Lab Biosensing, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, 189 Songling Rd, Qingdao 266101, Peoples R China
4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, 189 Songling Rd, Qingdao 266101, Peoples R China
5.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
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Feng, Ruirui,Liang, Bo,Hou, Chuantao,et al. Rational design of xylose dehydrogenase for improved thermostability and its application in development of efficient enzymatic biofuel cell[J]. ENZYME AND MICROBIAL TECHNOLOGY,2016,84:78-85.
APA Feng, Ruirui.,Liang, Bo.,Hou, Chuantao.,Han, Dongfei.,Han, Lei.,...&Han, Lihui.(2016).Rational design of xylose dehydrogenase for improved thermostability and its application in development of efficient enzymatic biofuel cell.ENZYME AND MICROBIAL TECHNOLOGY,84,78-85.
MLA Feng, Ruirui,et al."Rational design of xylose dehydrogenase for improved thermostability and its application in development of efficient enzymatic biofuel cell".ENZYME AND MICROBIAL TECHNOLOGY 84(2016):78-85.
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