| Low stability of the reduced state of Mycobacterium tuberculosis NrdH redoxin | |
Liu, Ya-Jun1,2,3 ; Song, Xiaxia4; Li, Yifei1,2,3; Xuan, Jinsong4; Cui, Qiu1,2,3; Wang, Jinfeng5; Feng, Yingang1,2,3
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| 2016-02-01 | |
| 发表期刊 | FEBS LETTERS
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| 卷号 | 590期号:3页码:387-395 |
| 摘要 | NrdH redoxin is the only hydrogen donor for ribonucleotide reductase in Mycobacterium tuberculosis. Several crystal structures of NrdH redoxins in the oxidized state from different species have been reported, but no structure of the reduced state has yet been reported. Using NMR spectroscopy, we found surprisingly that the reduced NrdH redoxin from M. tuberculosis is largely unfolded at a pH lower than the pKa of its first active site cysteine, and the structural basis of the low stability was analyzed. In addition, a single mutant of the NrdH redoxin suitable to determine the structure in the reduced state was obtained. |
| 文章类型 | Article |
| 关键词 | Mycobacterium Tuberculosis Nmr Nrdh Redoxin Protein Stability Protein Structure |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1002/1873-3468.12068 |
| 关键词[WOS] | N-15 NMR RELAXATION ; ESCHERICHIA-COLI ; ACTIVITY PROFILE ; PROTEIN ; C-13 ; DYNAMICS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| 项目资助者 | National Natural Science Foundation of China(31170701 ; 31300635) |
| WOS类目 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| WOS记录号 | WOS:000370136300009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/8008 |
| 专题 | 代谢物组学研究组 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Synthet Biol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Lab Single Cell Oil, Beijing 100864, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Beijing 100864, Peoples R China 4.Univ Sci & Technol Beijing, Sch Chem & Biol Engn, Dept Biol Sci & Engn, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Ya-Jun,Song, Xiaxia,Li, Yifei,et al. Low stability of the reduced state of Mycobacterium tuberculosis NrdH redoxin[J]. FEBS LETTERS,2016,590(3):387-395. |
| APA | Liu, Ya-Jun.,Song, Xiaxia.,Li, Yifei.,Xuan, Jinsong.,Cui, Qiu.,...&Feng, Yingang.(2016).Low stability of the reduced state of Mycobacterium tuberculosis NrdH redoxin.FEBS LETTERS,590(3),387-395. |
| MLA | Liu, Ya-Jun,et al."Low stability of the reduced state of Mycobacterium tuberculosis NrdH redoxin".FEBS LETTERS 590.3(2016):387-395. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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