Defluviitalea phaphyphila sp nov., a Novel Thermophilic Bacterium That Degrades Brown Algae

doi:10.1128/AEM.03297-15

QIBEBT-IR > 分子微生物工程研究组

	Defluviitalea phaphyphila sp nov., a Novel Thermophilic Bacterium That Degrades Brown Algae
	Ji, Shi-Qi; Wang, Bing; Lu, Ming; Li, Fu-Li
	2016-02-01
发表期刊	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷号	82 期号:3 页码:868-877
摘要	Brown algae are one of the largest groups of oceanic primary producers for CO2 removal and carbon sinks for coastal regions. However, the mechanism for brown alga assimilation remains largely unknown in thermophilic microorganisms. In this work, a thermophilic alginolytic community was enriched from coastal sediment, from which an obligate anaerobic and thermophilic bacterial strain, designated Alg1, was isolated. Alg1 shared a 16S rRNA gene identity of 94.6% with Defluviitalea saccharophila LIND6LT2(T). Phenotypic, chemotaxonomic, and phylogenetic studies suggested strain Alg1 represented a novel species of the genus Defluviitalea, for which the name Defluviitalea phaphyphila sp. nov. is proposed. Alg1 exhibited an intriguing ability to convert carbohydrates of brown algae, including alginate, laminarin, and mannitol, to ethanol and acetic acid. Three gene clusters participating in this process were predicted to be in the genome, and candidate enzymes were successfully expressed, purified, and characterized. Six alginate lyases were demonstrated to synergistically deconstruct alginate into unsaturated monosaccharide, followed by one uronic acid reductase and two 2-keto-3-deoxy-D-gluconate (KDG) kinases to produce pyruvate. A nonclassical mannitol 1-phosphate dehydrogenase, catalyzing D-mannitol 1-phosphate to fructose 1-phosphate in the presence of NAD(+), and one laminarase also were disclosed. This work revealed that a thermophilic brown alga-decomposing system containing numerous novel thermophilic alginate lyases and a unique mannitol 1-phosphate dehydrogenase was adopted by the natural ethanologenic strain Alg1 during the process of evolution in hostile habitats.
文章类型	Article
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1128/AEM.03297-15
关键词[WOS]	4-DEOXY-L-ERYTHRO-5-HEXOSEULOSE URONIC ACID ; OLIGOALGINATE LYASE ; ALGINATE LYASE ; GEN. NOV. ; BIOFUEL PRODUCTION ; CRYSTAL-STRUCTURE ; ESCHERICHIA-COLI ; MARINE SEDIMENT ; DEPOLYMERIZATION ; METABOLISM
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
项目资助者	National Natural Science Foundation of China (NSFC)(41506155) ; Science and Technology Development Project of Shandong Province(2014GHY115027) ; Shandong Province Natural Science Funds for Distinguished Young Scholar(JQ201507) ; Qingdao Institute of Bioenergy and Bioprocess Technology Director Innovation Foundation for Young Scientists(Y37207210B)
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:000369373800011
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/7971
专题	分子微生物工程研究组
作者单位	Chinese Acad Sci, Key Lab Biofuels, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Ji, Shi-Qi,Wang, Bing,Lu, Ming,et al. Defluviitalea phaphyphila sp nov., a Novel Thermophilic Bacterium That Degrades Brown Algae[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2016,82(3):868-877.
APA	Ji, Shi-Qi,Wang, Bing,Lu, Ming,&Li, Fu-Li.(2016).Defluviitalea phaphyphila sp nov., a Novel Thermophilic Bacterium That Degrades Brown Algae.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,82(3),868-877.
MLA	Ji, Shi-Qi,et al."Defluviitalea phaphyphila sp nov., a Novel Thermophilic Bacterium That Degrades Brown Algae".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 82.3(2016):868-877.