Antibiofilm and Anti-Infection of a Marine Bacterial Exopolysaccharide Against Pseudomonas aeruginosa

doi:10.3389/fmicb.2016.00102

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	Antibiofilm and Anti-Infection of a Marine Bacterial Exopolysaccharide Against Pseudomonas aeruginosa
	Wu, Shimei 1; Liu, Ge 2,3,4; Jin, Weihua 2,3; Xiu, Pengyuan 2,3,4; Sun, Chaomin 2,3
	2016-02-08
发表期刊	FRONTIERS IN MICROBIOLOGY
卷号	7
摘要	Pseudomonas aeruginosa is a well-known pathogenic bacterium that forms biofilms and produces virulence factors, thus leading to major problems in many fields, such as clinical infection, food contamination, and marine biofouling. In this study, we report the purification and characterization of an exopolysaccharide EPS273 from the culture supernatant of marine bacterium P. stutzeri 273. The exopolysaccharide EPS273 not only effectively inhibits biofilm formation but also disperses preformed biofilm of P aeruginosa PAO1. High performance liquid chromatography traces of the hydrolyzed polysaccharides shows that EPS273 primarily consists of glucosamine, rhamnose, glucose and mannose. Further investigation demonstrates that EPS273 reduces the production of the virulence factors pyocyanin, exoprotease, and rhamnolipid, and the virulence of P aeruginosa PAO1 to human lung cells A549 and zebrafish embryos is also obviously attenuated by EPS273. In addition, EPS273 also greatly reduces the production of hydrogen peroxide (H2O2) and extracellular DNA (eDNA), which are important factors for biofilm formation. Furthermore, EPS273 exhibits strong antioxidant potential by quenching hydroxyl and superoxide anion radicals. Notably, the antibiofouling activity of EPS273 is observed in the marine environment up to 2 weeks according to the amounts of bacteria and diatoms in the glass slides submerged in the ocean. Taken together, the properties of EPS273 indicate that it has a promising prospect in combating bacterial biofilm-associated infection, food-processing contamination and marine biofouling.
文章类型	Article
关键词	Antibiofilm Anti-infection Marine Exopolysaccharide Pseudomonas
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.3389/fmicb.2016.00102
关键词[WOS]	SPECTRUM BIOFILM INHIBITION ; EXTRACELLULAR DNA ; VIRULENCE ; PURIFICATION ; ANTIOXIDANT ; INFECTION ; PRODUCTS ; PROTEASE ; STRAINS ; FOOD
收录类别	SCI
语种	英语
WOS研究方向	Microbiology
项目资助者	Key Laboratory of Experimental Marine Biology Institute of Oceanology, Chinese Academy of Sciences ; "100-Talent Project" of Chinese Academy of Sciences(Y52424101L)
WOS类目	Microbiology
WOS记录号	WOS:000369474800001
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/7964
专题	仿生智能材料研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wu, Shimei,Liu, Ge,Jin, Weihua,et al. Antibiofilm and Anti-Infection of a Marine Bacterial Exopolysaccharide Against Pseudomonas aeruginosa[J]. FRONTIERS IN MICROBIOLOGY,2016,7.
APA	Wu, Shimei,Liu, Ge,Jin, Weihua,Xiu, Pengyuan,&Sun, Chaomin.(2016).Antibiofilm and Anti-Infection of a Marine Bacterial Exopolysaccharide Against Pseudomonas aeruginosa.FRONTIERS IN MICROBIOLOGY,7.
MLA	Wu, Shimei,et al."Antibiofilm and Anti-Infection of a Marine Bacterial Exopolysaccharide Against Pseudomonas aeruginosa".FRONTIERS IN MICROBIOLOGY 7(2016).