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Genomic Foundation of Starch-to-Lipid Switch in Oleaginous Chlorella spp.
Fan, Jianhua1; Ning, Kang2,3; Zeng, Xiaowei2,3; Luo, Yuanchan1; Wang, Dongmei2,3; Hu, Jianqiang2,3; Li, Jing2,3; Xu, Hui1; Huang, Jianke1; Wan, Minxi1; Wang, Weiliang; Zhang, Daojing1; Shen, Guomin1; Run, Conglin1; Liao, Junjie1; Fang, Lei1; Huang, Shi2,3; Jing, Xiaoyan2,3; Su, Xiaoquan2,3; Wang, Anhui2,3; Bai, Lili4; Hu, Zanmin4; Xu, Jian2,3; Li, Yuanguang1
2015-12-01
Source PublicationPLANT PHYSIOLOGY
Volume169Issue:4Pages:2444-2461
Abstract

The ability to rapidly switch the intracellular energy storage form from starch to lipids is an advantageous trait for microalgae feedstock. To probe this mechanism, we sequenced the 56.8-Mbp genome of Chlorella pyrenoidosa FACHB-9, an industrial production strain for protein, starch, and lipids. The genome exhibits positive selection and gene family expansion in lipid and carbohydrate metabolism and genes related to cell cycle and stress response. Moreover, 10 lipid metabolism genes might be originated from bacteria via horizontal gene transfer. Transcriptomic dynamics tracked via messenger RNA sequencing over six time points during metabolic switch from starch-rich heterotrophy to lipid-rich photoautotrophy revealed that under heterotrophy, genes most strongly expressed were from the tricarboxylic acid cycle, respiratory chain, oxidative phosphorylation, gluconeogenesis, glyoxylate cycle, and amino acid metabolisms, whereas those most down-regulated were from fatty acid and oxidative pentose phosphate metabolism. The shift from heterotrophy into photoautotrophy highlights up-regulation of genes from carbon fixation, photosynthesis, fatty acid biosynthesis, the oxidative pentose phosphate pathway, and starch catabolism, which resulted in a marked redirection of metabolism, where the primary carbon source of glycine is no longer supplied to cell building blocks by the tricarboxylic acid cycle and gluconeogenesis, whereas carbon skeletons from photosynthesis and starch degradation may be directly channeled into fatty acid and protein biosynthesis. By establishing the first genetic transformation in industrial oleaginous C. pyrenoidosa, we further showed that overexpression of an NAD(H) kinase from Arabidopsis (Arabidopsis thaliana) increased cellular lipid content by 110.4%, yet without reducing growth rate. These findings provide a foundation for exploiting the metabolic switch in microalgae for improved photosynthetic production of food and fuels.

SubtypeArticle
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1104/pp.15.01174
WOS KeywordTRANSCRIPTION FACTOR ; RNA-SEQ ; TRIACYLGLYCEROL ACCUMULATION ; CHLAMYDOMONAS-REINHARDTII ; HETEROTROPHIC CONDITIONS ; HAEMATOCOCCUS-PLUVIALIS ; COMMERCIAL APPLICATIONS ; NITROGEN DEPRIVATION ; MICROALGAL BIOFUELS ; ARABIDOPSIS PLANTS
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences
Funding OrganizationNational Basic Research Program of China(2011CB200904 ; National Natural Science Foundation of China(31300295 ; Chenguang Program by Shanghai Education Development Foundation and Shanghai Municipal Education Commission ; National Special Fund for State Key Laboratory of Bioreactor Engineering(2060204) ; National Key Technologies Research and Development Program of China(2011BAD14B02 ; Fundamental Research Funds for the Central Universities ; Open Funding Project of the State Key Laboratory of Bioreactor Engineering ; 2012CB721101) ; 31010103907 ; 2011BAD23B04) ; 31271410)
WOS SubjectPlant Sciences
WOS IDWOS:000368472700009
Citation statistics
Cited Times:48[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/7927
Collection单细胞中心
Affiliation1.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China
3.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China
4.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
Recommended Citation
GB/T 7714
Fan, Jianhua,Ning, Kang,Zeng, Xiaowei,et al. Genomic Foundation of Starch-to-Lipid Switch in Oleaginous Chlorella spp.[J]. PLANT PHYSIOLOGY,2015,169(4):2444-2461.
APA Fan, Jianhua.,Ning, Kang.,Zeng, Xiaowei.,Luo, Yuanchan.,Wang, Dongmei.,...&Li, Yuanguang.(2015).Genomic Foundation of Starch-to-Lipid Switch in Oleaginous Chlorella spp..PLANT PHYSIOLOGY,169(4),2444-2461.
MLA Fan, Jianhua,et al."Genomic Foundation of Starch-to-Lipid Switch in Oleaginous Chlorella spp.".PLANT PHYSIOLOGY 169.4(2015):2444-2461.
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