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Inhibition of Starch Synthesis Results in Overproduction of Lipids in Chlamydomonas reinhardtii
Li, Yantao1; Han, Danxiang1; Hu, Guangrong1,2; Sommerfeld, Milton1; Hu, Qiang1
2010-10-01
Source PublicationBIOTECHNOLOGY AND BIOENGINEERING
Volume107Issue:2Pages:258-268
AbstractStarch and neutral lipids are two major carbon storage compounds in many microalgae and plants. Lipids are more energy rich and have often been used as food and fuel feedstocks. Genetic engineering of the lipid biosynthesis pathway to overproduce lipid has achieved only limited success. We hypothesize that through blocking the competing pathway to produce starch, overproduction of neutral lipid may be achieved. This hypothesis was tested using the green microalga Chlamydomonas reinhardtii and its low starch and starchless mutants. We discovered that a dramatic increase in neutral lipid content and the neutral lipid/total lipid ratio occurred among the mutants under high light and nitrogen starvation. BAFJ5, one of the mutants defective in the small subunit of ADP-glucose pyrophosphorylase, accumulated neutral and total lipid of up to 32.6% and 46.4% of dry weight (DW) or 8- and 3.5-fold higher, respectively, than the wild-type. These results confirmed the feasibility of increasing lipid production through redirecting photosynthetically assimilated carbon away from starch synthesis to neutral lipid synthesis. However, some growth impairment was observed in the low starch and starchless mutants, possibly due to altered energy partitioning in PSII, with more excitation energy dissipated as heat and less to photochemical conversion. This study demonstrated that biomass and lipid production by the selected mutants can be improved by physiological manipulation. Biotechnol. Bioeng. 2010;107: 258-268. (c) 2010 Wiley Periodicals, Inc.; Starch and neutral lipids are two major carbon storage compounds in many microalgae and plants. Lipids are more energy rich and have often been used as food and fuel feedstocks. Genetic engineering of the lipid biosynthesis pathway to overproduce lipid has achieved only limited success. We hypothesize that through blocking the competing pathway to produce starch, overproduction of neutral lipid may be achieved. This hypothesis was tested using the green microalga Chlamydomonas reinhardtii and its low starch and starchless mutants. We discovered that a dramatic increase in neutral lipid content and the neutral lipid/total lipid ratio occurred among the mutants under high light and nitrogen starvation. BAFJ5, one of the mutants defective in the small subunit of ADP-glucose pyrophosphorylase, accumulated neutral and total lipid of up to 32.6% and 46.4% of dry weight (DW) or 8- and 3.5-fold higher, respectively, than the wild-type. These results confirmed the feasibility of increasing lipid production through redirecting photosynthetically assimilated carbon away from starch synthesis to neutral lipid synthesis. However, some growth impairment was observed in the low starch and starchless mutants, possibly due to altered energy partitioning in PSII, with more excitation energy dissipated as heat and less to photochemical conversion. This study demonstrated that biomass and lipid production by the selected mutants can be improved by physiological manipulation. Biotechnol. Bioeng. 2010;107: 258-268. (c) 2010 Wiley Periodicals, Inc.
SubtypeArticle
KeywordBiofuel High Light Lipid Microalgae Nitrogen Starvation Starch
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1002/bit.22807
WOS KeywordSEED OIL CONTENT ; ADP-GLUCOSE PYROPHOSPHORYLASE ; RAPE BRASSICA-NAPUS ; OILSEED RAPE ; FATTY-ACID ; DIACYLGLYCEROL ACYLTRANSFERASE ; ALPHA-1,4 GLUCANOTRANSFERASES ; CHLOROPHYLL FLUORESCENCE ; NICOTIANA-SYLVESTRIS ; METABOLIC-CONTROL
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000281857500007
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/714
Collection微生物资源团队
Affiliation1.Arizona State Univ, Dept Appl Sci & Math, Mesa, AZ 85212 USA
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Li, Yantao,Han, Danxiang,Hu, Guangrong,et al. Inhibition of Starch Synthesis Results in Overproduction of Lipids in Chlamydomonas reinhardtii[J]. BIOTECHNOLOGY AND BIOENGINEERING,2010,107(2):258-268.
APA Li, Yantao,Han, Danxiang,Hu, Guangrong,Sommerfeld, Milton,&Hu, Qiang.(2010).Inhibition of Starch Synthesis Results in Overproduction of Lipids in Chlamydomonas reinhardtii.BIOTECHNOLOGY AND BIOENGINEERING,107(2),258-268.
MLA Li, Yantao,et al."Inhibition of Starch Synthesis Results in Overproduction of Lipids in Chlamydomonas reinhardtii".BIOTECHNOLOGY AND BIOENGINEERING 107.2(2010):258-268.
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