| Inhibition of Starch Synthesis Results in Overproduction of Lipids in Chlamydomonas reinhardtii | |
Li, Yantao1; Han, Danxiang1; Hu, Guangrong1,2 ; Sommerfeld, Milton1; Hu, Qiang1
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| 2010-10-01 | |
| Source Publication | BIOTECHNOLOGY AND BIOENGINEERING
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| Volume | 107Issue:2Pages:258-268 |
| Abstract | Starch and neutral lipids are two major carbon storage compounds in many microalgae and plants. Lipids are more energy rich and have often been used as food and fuel feedstocks. Genetic engineering of the lipid biosynthesis pathway to overproduce lipid has achieved only limited success. We hypothesize that through blocking the competing pathway to produce starch, overproduction of neutral lipid may be achieved. This hypothesis was tested using the green microalga Chlamydomonas reinhardtii and its low starch and starchless mutants. We discovered that a dramatic increase in neutral lipid content and the neutral lipid/total lipid ratio occurred among the mutants under high light and nitrogen starvation. BAFJ5, one of the mutants defective in the small subunit of ADP-glucose pyrophosphorylase, accumulated neutral and total lipid of up to 32.6% and 46.4% of dry weight (DW) or 8- and 3.5-fold higher, respectively, than the wild-type. These results confirmed the feasibility of increasing lipid production through redirecting photosynthetically assimilated carbon away from starch synthesis to neutral lipid synthesis. However, some growth impairment was observed in the low starch and starchless mutants, possibly due to altered energy partitioning in PSII, with more excitation energy dissipated as heat and less to photochemical conversion. This study demonstrated that biomass and lipid production by the selected mutants can be improved by physiological manipulation. Biotechnol. Bioeng. 2010;107: 258-268. (c) 2010 Wiley Periodicals, Inc.; Starch and neutral lipids are two major carbon storage compounds in many microalgae and plants. Lipids are more energy rich and have often been used as food and fuel feedstocks. Genetic engineering of the lipid biosynthesis pathway to overproduce lipid has achieved only limited success. We hypothesize that through blocking the competing pathway to produce starch, overproduction of neutral lipid may be achieved. This hypothesis was tested using the green microalga Chlamydomonas reinhardtii and its low starch and starchless mutants. We discovered that a dramatic increase in neutral lipid content and the neutral lipid/total lipid ratio occurred among the mutants under high light and nitrogen starvation. BAFJ5, one of the mutants defective in the small subunit of ADP-glucose pyrophosphorylase, accumulated neutral and total lipid of up to 32.6% and 46.4% of dry weight (DW) or 8- and 3.5-fold higher, respectively, than the wild-type. These results confirmed the feasibility of increasing lipid production through redirecting photosynthetically assimilated carbon away from starch synthesis to neutral lipid synthesis. However, some growth impairment was observed in the low starch and starchless mutants, possibly due to altered energy partitioning in PSII, with more excitation energy dissipated as heat and less to photochemical conversion. This study demonstrated that biomass and lipid production by the selected mutants can be improved by physiological manipulation. Biotechnol. Bioeng. 2010;107: 258-268. (c) 2010 Wiley Periodicals, Inc. |
| Subtype | Article |
| Keyword | Biofuel High Light Lipid Microalgae Nitrogen Starvation Starch |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1002/bit.22807 |
| WOS Keyword | SEED OIL CONTENT ; ADP-GLUCOSE PYROPHOSPHORYLASE ; RAPE BRASSICA-NAPUS ; OILSEED RAPE ; FATTY-ACID ; DIACYLGLYCEROL ACYLTRANSFERASE ; ALPHA-1,4 GLUCANOTRANSFERASES ; CHLOROPHYLL FLUORESCENCE ; NICOTIANA-SYLVESTRIS ; METABOLIC-CONTROL |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biotechnology & Applied Microbiology |
| WOS Subject | Biotechnology & Applied Microbiology |
| WOS ID | WOS:000281857500007 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.qibebt.ac.cn/handle/337004/714 |
| Collection | 分子微生物工程研究组 |
| Affiliation | 1.Arizona State Univ, Dept Appl Sci & Math, Mesa, AZ 85212 USA 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Shandong, Peoples R China |
| Recommended Citation GB/T 7714 | Li, Yantao,Han, Danxiang,Hu, Guangrong,et al. Inhibition of Starch Synthesis Results in Overproduction of Lipids in Chlamydomonas reinhardtii[J]. BIOTECHNOLOGY AND BIOENGINEERING,2010,107(2):258-268. |
| APA | Li, Yantao,Han, Danxiang,Hu, Guangrong,Sommerfeld, Milton,&Hu, Qiang.(2010).Inhibition of Starch Synthesis Results in Overproduction of Lipids in Chlamydomonas reinhardtii.BIOTECHNOLOGY AND BIOENGINEERING,107(2),258-268. |
| MLA | Li, Yantao,et al."Inhibition of Starch Synthesis Results in Overproduction of Lipids in Chlamydomonas reinhardtii".BIOTECHNOLOGY AND BIOENGINEERING 107.2(2010):258-268. |
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