| Sustained photo-hydrogen production by Chlorella pyrenoidosa without sulfur depletion | |
Wang, Huanyu1,2; Fan, Xiaolei1 ; Zhang, Yanting1,2; Yang, Dawei1,2; Guo, Rongbo1
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| 2011-07-01 | |
| 发表期刊 | BIOTECHNOLOGY LETTERS
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| ISSN | 0141-5492 |
| 卷号 | 33期号:7页码:1345-1350 |
| 摘要 | The sustained production of H(2) by Chlorella pyrenoidosa was achieved without sulfur deficiency or PSII inhibition. C. pyrenoidosa preserved hydrogenase activity for several hours in the dark. Hydrogenase activity in vitro is O(2) sensitive, which indicates that respiration may play an important role in H(2) production. A sustainable production of H(2) was obtained for 200 h under illumination. Further, by using 3-(3,4-dichlorophenyl)-1,1-dimethylurea as a PSII inhibitor, at least 70% electrons for H(2) production were generated from PSII-catalyzed-H(2)O oxidation. The remaining electrons were probably from endogenous substrate degradation.; The sustained production of H(2) by Chlorella pyrenoidosa was achieved without sulfur deficiency or PSII inhibition. C. pyrenoidosa preserved hydrogenase activity for several hours in the dark. Hydrogenase activity in vitro is O(2) sensitive, which indicates that respiration may play an important role in H(2) production. A sustainable production of H(2) was obtained for 200 h under illumination. Further, by using 3-(3,4-dichlorophenyl)-1,1-dimethylurea as a PSII inhibitor, at least 70% electrons for H(2) production were generated from PSII-catalyzed-H(2)O oxidation. The remaining electrons were probably from endogenous substrate degradation. |
| 文章类型 | Article |
| 关键词 | Chlorella Pyrenoidosa Hydrogenase Photo-hydrogen Production |
| 学科领域 | 生物制氢与沼气 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1007/s10529-011-0584-x |
| 关键词[WOS] | ALGA PLATYMONAS-SUBCORDIFORMIS ; CHLAMYDOMONAS-REINHARDTII ; GREEN-ALGA ; PHOTOPRODUCTION ; ACETATE ; EVOLUTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000291655700009 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/700 |
| 专题 | 工业生物燃气研究组 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Huanyu,Fan, Xiaolei,Zhang, Yanting,et al. Sustained photo-hydrogen production by Chlorella pyrenoidosa without sulfur depletion[J]. BIOTECHNOLOGY LETTERS,2011,33(7):1345-1350. |
| APA | Wang, Huanyu,Fan, Xiaolei,Zhang, Yanting,Yang, Dawei,&Guo, Rongbo.(2011).Sustained photo-hydrogen production by Chlorella pyrenoidosa without sulfur depletion.BIOTECHNOLOGY LETTERS,33(7),1345-1350. |
| MLA | Wang, Huanyu,et al."Sustained photo-hydrogen production by Chlorella pyrenoidosa without sulfur depletion".BIOTECHNOLOGY LETTERS 33.7(2011):1345-1350. |
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