Purification and biochemical characterization of an alkaline protease from marine bacteria Pseudoalteromonas sp 129-1

doi:10.1002/jobm.201500327

QIBEBT-IR > 仿生智能材料研究组

	Purification and biochemical characterization of an alkaline protease from marine bacteria Pseudoalteromonas sp 129-1
	Wu, Shimei 1; Liu, Ge 2; Zhang, Dechao 3; Li, Chaoxu 1; Sun, Chaomin 2
	2015-12-01
发表期刊	JOURNAL OF BASIC MICROBIOLOGY
卷号	55 期号:12 页码:1427-1434
摘要	An extracellular alkaline protease produced by marine bacteria strain Pseudoalteromonas sp. 129-1 was purified by ammonium sulphate precipitation, anion exchange chromatography, and gel filtration. The purity of the protease was confirmed by SDS-PAGE and molecular mass was estimated to be 35kDa. The protease maintained considerable activity and stability at a wide temperature range of 10-60 degrees C and pH range of 6-11, and optimum activity was detected at temperature of 50 degrees C and pH of 8. Metallo-protease inhibitor, EDTA, had no inhibitory effect on protease activity even at concentration up to 15mM, whereas 15mM PMSF, a common serine protease inhibitor, greatly inactivated the protease. The high stability of the protease in the presence of surfactants (SDS, Tween 80, and Triton X-100), oxidizing agent H2O2, and commercial detergents was observed. Moreover, the protease was tolerant to most of the tested organic solvents, and saline tolerant up to 30%. Interestingly, biofilm of Pseudomonas aeruginosa PAO1 was greatly reduced by 0.01mgml(-1) of the protease, and nearly completely abolished with the concentration of 1mgml(-1). Collectively, the protease showed valuable feathers as an additive in laundry detergent and non-toxic anti-biofilm agent.
文章类型	Article
关键词	Pseudoalteromonas Alkaline Protease Purification Surfactant Biofilm
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1002/jobm.201500327
关键词[WOS]	SALT-TOLERANT ; ENZYMES ; BIOTECHNOLOGY ; SUBTILISIN
收录类别	SCI
语种	英语
WOS研究方向	Microbiology
项目资助者	"100-Talent Project" of Chinese Academy of Sciences(Y42416101Q) ; National Natural Science Foundation of China(21474125 ; 31470181)
WOS类目	Microbiology
WOS记录号	WOS:000366143300009
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6816
专题	仿生智能材料研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Wu, Shimei,Liu, Ge,Zhang, Dechao,et al. Purification and biochemical characterization of an alkaline protease from marine bacteria Pseudoalteromonas sp 129-1[J]. JOURNAL OF BASIC MICROBIOLOGY,2015,55(12):1427-1434.
APA	Wu, Shimei,Liu, Ge,Zhang, Dechao,Li, Chaoxu,&Sun, Chaomin.(2015).Purification and biochemical characterization of an alkaline protease from marine bacteria Pseudoalteromonas sp 129-1.JOURNAL OF BASIC MICROBIOLOGY,55(12),1427-1434.
MLA	Wu, Shimei,et al."Purification and biochemical characterization of an alkaline protease from marine bacteria Pseudoalteromonas sp 129-1".JOURNAL OF BASIC MICROBIOLOGY 55.12(2015):1427-1434.