CRGO/alginate microbeads: an enzyme immobilization system and its potential application for a continuous enzymatic reaction

doi:10.1039/c5tb01508a

QIBEBT-IR > 绿色化学催化研究组

	CRGO/alginate microbeads: an enzyme immobilization system and its potential application for a continuous enzymatic reaction
	Zhao, Fuhua 1; Li, Hui 1; Wang, Xicheng 1; Wu, Lin 2; Hou, Tonggang 1; Guan, Jing 1; Jiang, Yijun 1; Xu, Huanfei 1; Mu, Xindong 1
	2015
发表期刊	JOURNAL OF MATERIALS CHEMISTRY B
卷号	3 期号:48 页码:9315-9322
摘要	In this work, novel hybrid microbeads composed of chemically reduced graphene oxide (CRGO) and alginate were fabricated, which could encapsulate enzymes by a simple non-covalent adsorption-entrapment method. Compared with alginate gel beads, the intervention of CRGO in the alginate gel enhanced its mechanical strength, effectively prevented the leakage of enzyme, and greatly enhanced the stability and environmental tolerance. Compared with free enzymes or those on a single carrier, the enzyme encapsulated in these hybrid microbeads can retain its optimum activity within a broad range (temperature 45-60 degrees C, pH 4-6). Additionally, the microbeads can be easily recycled by simple filtration and filled into a column to achieve a continuous fixed-bed enzyme catalytic reaction.
文章类型	Article
WOS标题词	Science & Technology ; Technology
DOI	10.1039/c5tb01508a
关键词[WOS]	REDUCED GRAPHENE OXIDE ; INORGANIC HYBRID NANOFLOWERS ; CA-ALGINATE BEADS ; GLUCOSE-OXIDASE ; ENCAPSULATION ; SILICA ; PERFORMANCE ; ENTRAPMENT ; STABILITY ; CAPSULES
收录类别	SCI
语种	英语
WOS研究方向	Materials Science
项目资助者	K. C. Wong Education Foundation ; Youth Innovation Promotion Association of CAS ; Qingdao Institute of Bioenergy and Bioprocess Technology Director Technology Foundation ; Shandong Provincial Natural Science Foundation for Distinguished Young Scholar, China(JQ201305)
WOS类目	Materials Science, Biomaterials
WOS记录号	WOS:000366163900010
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6811
专题	绿色化学催化研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biobased Mat, Qingdao 266101, Peoples R China 2.Qingdao Tech Coll, Qingdao 266000, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Fuhua,Li, Hui,Wang, Xicheng,et al. CRGO/alginate microbeads: an enzyme immobilization system and its potential application for a continuous enzymatic reaction[J]. JOURNAL OF MATERIALS CHEMISTRY B,2015,3(48):9315-9322.
APA	Zhao, Fuhua.,Li, Hui.,Wang, Xicheng.,Wu, Lin.,Hou, Tonggang.,...&Mu, Xindong.(2015).CRGO/alginate microbeads: an enzyme immobilization system and its potential application for a continuous enzymatic reaction.JOURNAL OF MATERIALS CHEMISTRY B,3(48),9315-9322.
MLA	Zhao, Fuhua,et al."CRGO/alginate microbeads: an enzyme immobilization system and its potential application for a continuous enzymatic reaction".JOURNAL OF MATERIALS CHEMISTRY B 3.48(2015):9315-9322.