| Combinatory strategy for characterizing and understanding the ethanol synthesis pathway in cyanobacteria cell factories | |
Luan, Guodong1,2 ; Qi, Yunjing4; Wang, Min1,3; Li, Zhimin1,2; Duan, Yangkai1,3,5; Tan, Xiaoming1,2; Lu, Xuefeng1,2
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| 2015-11-21 | |
| 发表期刊 | BIOTECHNOLOGY FOR BIOFUELS
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| 卷号 | 8 |
| 摘要 | Background: Photosynthetic production of chemicals and fuels by recycling CO2 in cyanobacteria is a promising solution facing energy shortage and resource declination. Ethanol is an attractive and demonstrative biofuel product, and ethanol synthesis in cyanobacteria has been achieved by assembling of a pathway consisting of pyruvate decarboxylase (PDCzm) and alcohol dehydrogenase II (slr1192). For enabling more powerful ethanol photosynthetic production, an optimized and balanced catalyzing route was required. In this work, we provided a paradigm for systematically characterizing and optimizing the PDCzm-slr1192 pathway from engineered cyanobacteria strains, combining in vitro reconstitution, genetic engineering and feeding-cultivation. |
| 文章类型 | Article |
| 关键词 | In Vitro Reconstitution Steady-state Analysis Ethanol Cyanobacteria Metabolic Engineering |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| DOI | 10.1186/s13068-015-0367-z |
| 关键词[WOS] | SYNECHOCYSTIS SP PCC6803 ; SP PCC 6803 ; IN-VITRO RECONSTITUTION ; FATTY-ACID SYNTHASE ; CARBON-DIOXIDE ; PHOTOSYNTHETIC PRODUCTION ; ESCHERICHIA-COLI ; LACTIC-ACID ; ZYMOMONAS-MOBILIS ; 2,3-BUTANEDIOL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Energy & Fuels |
| 项目资助者 | National High-Tech Research and Development Program of China(2012AA052103) ; Excellent Youth Award of the Shandong Natural Science Foundation(JQ201306) ; Shandong Taishan Scholarship ; Shandong Postdoctoral Innovation Project(145071) ; Scientific Funds for Outstanding Young Scientists of Shandong Province(BS2014SW021) ; China Postdoctoral Science Foundation(2015M570616) |
| WOS类目 | Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS记录号 | WOS:000365783700001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/6800 |
| 专题 | 微生物代谢工程研究组 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, Qingdao 266101, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China 4.Qingdao Univ Sci & Technol, Qingdao 266061, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luan, Guodong,Qi, Yunjing,Wang, Min,et al. Combinatory strategy for characterizing and understanding the ethanol synthesis pathway in cyanobacteria cell factories[J]. BIOTECHNOLOGY FOR BIOFUELS,2015,8. |
| APA | Luan, Guodong.,Qi, Yunjing.,Wang, Min.,Li, Zhimin.,Duan, Yangkai.,...&Lu, Xuefeng.(2015).Combinatory strategy for characterizing and understanding the ethanol synthesis pathway in cyanobacteria cell factories.BIOTECHNOLOGY FOR BIOFUELS,8. |
| MLA | Luan, Guodong,et al."Combinatory strategy for characterizing and understanding the ethanol synthesis pathway in cyanobacteria cell factories".BIOTECHNOLOGY FOR BIOFUELS 8(2015). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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