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In vitro method for prediction of plaque reduction by dentifrice
Tepper, Bruce1; Howard, Brian2; Schnell, Daniel3; Mills, Lisa1; Xu, Jian4,5
2015-11-01
发表期刊JOURNAL OF MICROBIOLOGICAL METHODS
卷号118期号:1页码:85-92
摘要

An in vitro Particle Based Biofilm (PBB) model was developed to enable high throughput screening tests to predict clinical plaque reduction. Multi-species oral biofilms were cultured from pooled stimulated human saliva on continuously-colliding hydroxyapatite particles. After three days PBBs were saline washed prior to use in screening tests. Testing involved dosing PBBs for 1 min followed by neutralization of test materials and rinsing. PBBs were then assayed for intact biofilm activity measured as ATP. The ranking of commercial dentifrices from most to least reduction of intact biofilm activity was Crest ProHealth Clinical Gum Protection, Crest ProHealth, Colgate Total and Crest Cavity Protection. We demonstrated five advantages of the PBB model: 1) the ATP metric had a linear response over >= 1000-fold dynamic range, 2) potential interference with the ATP assay by treatments was easily eliminated by rinsing PBBs with saline, 3) discriminating power was statistically excellent between all treatment comparisons with the negative controls, 4) screening test results were reproducible across four tests, and 5) the screening test produced the same rank order for dentifrices as clinical studies that measured plaque reduction. In addition, 454 pyrosequencing of the PBBs indicated an oral microbial consortium was present. The most prevalent genera were Neisseria, Rothia, Streptococcus, Porphyromonas, Prevotella, Actinomyces, Fusobacterium, Veillonella and Haemophilus. We conclude these in vitro methods offer an efficient, effective and relevant screening tool for reduction of intact biofilm activity by dentifrices. Moreover, dentifrice rankings by the in vitro test method are expected to predict clinical results for plaque reduction. (C) 2015 Elsevier B.V. All rights reserved.

文章类型Article
关键词Oral Biofilm Biofilm Cleaning 454 Pyrosequencing
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.mimet.2015.06.017
关键词[WOS]COATED GLASS-BEADS ; HYDROXYAPATITE SURFACES ; MODEL SYSTEMS ; FLORA ; MOUTHRINSES ; VALIDATION ; CARIES
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Microbiology
项目资助者Procter Gamble Company
WOS类目Biochemical Research Methods ; Microbiology
WOS记录号WOS:000364892300015
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/6786
专题单细胞中心
作者单位1.Procter & Gamble Co, Microbiol Capabil Org, Mason, OH 45040 USA
2.Procter & Gamble Co, Life Sci Innovat, Mason, OH 45040 USA
3.Procter & Gamble Co, Stat & Data Management, Mason, OH 45040 USA
4.Chinese Acad Sci, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China
5.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China
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GB/T 7714
Tepper, Bruce,Howard, Brian,Schnell, Daniel,et al. In vitro method for prediction of plaque reduction by dentifrice[J]. JOURNAL OF MICROBIOLOGICAL METHODS,2015,118(1):85-92.
APA Tepper, Bruce,Howard, Brian,Schnell, Daniel,Mills, Lisa,&Xu, Jian.(2015).In vitro method for prediction of plaque reduction by dentifrice.JOURNAL OF MICROBIOLOGICAL METHODS,118(1),85-92.
MLA Tepper, Bruce,et al."In vitro method for prediction of plaque reduction by dentifrice".JOURNAL OF MICROBIOLOGICAL METHODS 118.1(2015):85-92.
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