中国科学院青岛生物能源与过程研究所机构知识库
Advanced  
QIBEBT-IR  > 单细胞中心  > 期刊论文
Title:
In vitro method for prediction of plaque reduction by dentifrice
Author: Tepper, Bruce1; Howard, Brian2; Schnell, Daniel3; Mills, Lisa1; Xu Jian(徐健)4,5
Source: JOURNAL OF MICROBIOLOGICAL METHODS
Issued Date: 2015-11-01
Volume: 118, Issue:1, Pages:85-92
Keyword: Oral biofilm ; Biofilm cleaning ; 454 pyrosequencing
DOI: 10.1016/j.mimet.2015.06.017
DOC Type: Article
English Abstract:

An in vitro Particle Based Biofilm (PBB) model was developed to enable high throughput screening tests to predict clinical plaque reduction. Multi-species oral biofilms were cultured from pooled stimulated human saliva on continuously-colliding hydroxyapatite particles. After three days PBBs were saline washed prior to use in screening tests. Testing involved dosing PBBs for 1 min followed by neutralization of test materials and rinsing. PBBs were then assayed for intact biofilm activity measured as ATP. The ranking of commercial dentifrices from most to least reduction of intact biofilm activity was Crest ProHealth Clinical Gum Protection, Crest ProHealth, Colgate Total and Crest Cavity Protection. We demonstrated five advantages of the PBB model: 1) the ATP metric had a linear response over >= 1000-fold dynamic range, 2) potential interference with the ATP assay by treatments was easily eliminated by rinsing PBBs with saline, 3) discriminating power was statistically excellent between all treatment comparisons with the negative controls, 4) screening test results were reproducible across four tests, and 5) the screening test produced the same rank order for dentifrices as clinical studies that measured plaque reduction. In addition, 454 pyrosequencing of the PBBs indicated an oral microbial consortium was present. The most prevalent genera were Neisseria, Rothia, Streptococcus, Porphyromonas, Prevotella, Actinomyces, Fusobacterium, Veillonella and Haemophilus. We conclude these in vitro methods offer an efficient, effective and relevant screening tool for reduction of intact biofilm activity by dentifrices. Moreover, dentifrice rankings by the in vitro test method are expected to predict clinical results for plaque reduction. (C) 2015 Elsevier B.V. All rights reserved.

WOS Headings: Science & Technology ; Life Sciences & Biomedicine
WOS Subject: Biochemical Research Methods ; Microbiology
WOS Subject Extended: Biochemistry & Molecular Biology ; Microbiology
WOS Keyword Plus: COATED GLASS-BEADS ; HYDROXYAPATITE SURFACES ; MODEL SYSTEMS ; FLORA ; MOUTHRINSES ; VALIDATION ; CARIES
Indexed Type: SCI
Funder: Procter Gamble Company
Language: 英语
WOS ID: WOS:000364892300015
Citation statistics:
Content Type: 期刊论文
URI: http://ir.qibebt.ac.cn/handle/337004/6786
Appears in Collections:单细胞中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
In vitro method for prediction of plaque reduction by dentifrice.pdf(754KB)期刊论文作者接受稿开放获取View Download

description.institution: 1.Procter & Gamble Co, Microbiol Capabil Org, Mason, OH 45040 USA
2.Procter & Gamble Co, Life Sci Innovat, Mason, OH 45040 USA
3.Procter & Gamble Co, Stat & Data Management, Mason, OH 45040 USA
4.Chinese Acad Sci, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China
5.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China

Recommended Citation:
Tepper, Bruce,Howard, Brian,Schnell, Daniel,et al. In vitro method for prediction of plaque reduction by dentifrice[J]. JOURNAL OF MICROBIOLOGICAL METHODS,2015,118(1):85-92.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Tepper, Bruce]'s Articles
[Howard, Brian]'s Articles
[Schnell, Daniel]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Tepper, Bruce]‘s Articles
[Howard, Brian]‘s Articles
[Schnell, Daniel]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: In vitro method for prediction of plaque reduction by dentifrice.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace