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Increasing fatty acid production in E. coli by simulating the lipid accumulation of oleaginous microorganisms
Meng, Xin1; Yang, Jianming1; Cao, Yujin1; Li, Liangzhi1; Jiang, Xinglin1; Xu, Xin1; Liu, Wei1; Xian, Mo1; Zhang, Yingwei2
2011-08-01
发表期刊JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
ISSN1367-5435
卷号38期号:8页码:919-925
摘要Unlike many oleaginous microorganisms, E. coli only maintains a small amount of natural lipids in cells, impeding its utility to overproduce fatty acids. In this study, acetyl-CoA carboxylase (ACC) from Acinetobacter calcoaceticus was expressed in E. coli to redirect the carbon flux to the generation of malonyl-CoA, which resulted in a threefold increase in intracellular lipids. Moreover, providing a high level of NADPH by overexpressing malic enzyme and adding malate to the culture medium resulted in a fourfold increase in intracellular lipids (about 197.74 mg/g). Co-expression of ACC and malic enzyme resulted in 284.56 mg/g intracellular lipids, a 5.6-fold increase compared to the wild-type strain. This study provides some attractive strategies for increasing lipid production in E. coli by simulating the lipid accumulation of oleaginous microorganisms, which could aid the development of a prokaryotic fatty acid producer.; Unlike many oleaginous microorganisms, E. coli only maintains a small amount of natural lipids in cells, impeding its utility to overproduce fatty acids. In this study, acetyl-CoA carboxylase (ACC) from Acinetobacter calcoaceticus was expressed in E. coli to redirect the carbon flux to the generation of malonyl-CoA, which resulted in a threefold increase in intracellular lipids. Moreover, providing a high level of NADPH by overexpressing malic enzyme and adding malate to the culture medium resulted in a fourfold increase in intracellular lipids (about 197.74 mg/g). Co-expression of ACC and malic enzyme resulted in 284.56 mg/g intracellular lipids, a 5.6-fold increase compared to the wild-type strain. This study provides some attractive strategies for increasing lipid production in E. coli by simulating the lipid accumulation of oleaginous microorganisms, which could aid the development of a prokaryotic fatty acid producer.
文章类型Article
关键词Fatty Acid Lipids Oleaginous Microorganisms Acc Nadp Me
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s10295-010-0861-z
关键词[WOS]COENZYME-A CARBOXYLASE ; CELL OIL PRODUCTION ; ESCHERICHIA-COLI ; MALIC ENZYME ; ACETYL-COA ; MUCOR-CIRCINELLOIDES ; OVERPRODUCTION ; BIOSYNTHESIS ; BIODIESEL ; MICROBES
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000293002000005
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/678
专题生物基材料组群
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuel, Qingdao, Peoples R China
2.Risun Coal Chem Grp Ltd, Xingtai, Peoples R China
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GB/T 7714
Meng, Xin,Yang, Jianming,Cao, Yujin,et al. Increasing fatty acid production in E. coli by simulating the lipid accumulation of oleaginous microorganisms[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2011,38(8):919-925.
APA Meng, Xin.,Yang, Jianming.,Cao, Yujin.,Li, Liangzhi.,Jiang, Xinglin.,...&Zhang, Yingwei.(2011).Increasing fatty acid production in E. coli by simulating the lipid accumulation of oleaginous microorganisms.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,38(8),919-925.
MLA Meng, Xin,et al."Increasing fatty acid production in E. coli by simulating the lipid accumulation of oleaginous microorganisms".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 38.8(2011):919-925.
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