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Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes
Wu, Wei1; Zhang, Lei1,2; Yao, Lun1,2; Tan, Xiaoming1; Liu, Xufeng1,2; Lu, Xuefeng1
2015-06-03
Source PublicationSCIENTIFIC REPORTS
Volume5
AbstractConstruction of highly efficient microbial cell factories producing drop-in biofuel alkanes is severely limited due to the lack of a fast detection method against alkanes. Here we first developed a sensitive fluorescent biosensor for rapid and in situ monitoring of intracellular alkane synthesis. Using GFP as reporter, the biosensor could actively respond to the intracellular alkane products, especially for the mid-and long-chain alkanes synthesized in the recombinant Escherichia coli and give a concentration-dependent fluorescence response. Our results also suggested the feasibility of developing high-throughput strategies basing on the alkane biosensor device in E. coli, and thus will greatly facilitate the application of directed evolution strategies to further improve the alkane-producing microbial cell factories.
SubtypeArticle
WOS HeadingsScience & Technology
DOI10.1038/srep10907
WOS KeywordSHORT-CHAIN ALKANES ; ESCHERICHIA-COLI ; EXPRESSION ; EVOLUTION
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
Funding OrganizationNational Basic Research Program of China (973)(2011CBA00907) ; National Natural Science Foundation of China(21406249) ; SOA project(GASI-03-01-02-05) ; China Postdoctoral Science Foundation(2014M551979) ; Joint Research Laboratory for Sustainable Aviation Biofuels (QIBEBT-Boeing)
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000364452000001
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/6776
Collection微生物代谢工程研究组
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Key Lab Biofuels, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Wu, Wei,Zhang, Lei,Yao, Lun,et al. Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes[J]. SCIENTIFIC REPORTS,2015,5.
APA Wu, Wei,Zhang, Lei,Yao, Lun,Tan, Xiaoming,Liu, Xufeng,&Lu, Xuefeng.(2015).Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes.SCIENTIFIC REPORTS,5.
MLA Wu, Wei,et al."Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes".SCIENTIFIC REPORTS 5(2015).
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