| Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes | |
| Wu, Wei1; Zhang, Lei1,2; Yao, Lun1,2; Tan, Xiaoming1; Liu, Xufeng1,2; Lu, Xuefeng1 | |
| 2015-06-03 | |
| 发表期刊 | SCIENTIFIC REPORTS
 |
| 卷号 | 5 |
| 摘要 | Construction of highly efficient microbial cell factories producing drop-in biofuel alkanes is severely limited due to the lack of a fast detection method against alkanes. Here we first developed a sensitive fluorescent biosensor for rapid and in situ monitoring of intracellular alkane synthesis. Using GFP as reporter, the biosensor could actively respond to the intracellular alkane products, especially for the mid-and long-chain alkanes synthesized in the recombinant Escherichia coli and give a concentration-dependent fluorescence response. Our results also suggested the feasibility of developing high-throughput strategies basing on the alkane biosensor device in E. coli, and thus will greatly facilitate the application of directed evolution strategies to further improve the alkane-producing microbial cell factories. |
| 文章类型 | Article |
| WOS标题词 | Science & Technology |
| DOI | 10.1038/srep10907 |
| 关键词[WOS] | SHORT-CHAIN ALKANES ; ESCHERICHIA-COLI ; EXPRESSION ; EVOLUTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Science & Technology - Other Topics |
| 项目资助者 | National Basic Research Program of China (973)(2011CBA00907) ; National Natural Science Foundation of China(21406249) ; SOA project(GASI-03-01-02-05) ; China Postdoctoral Science Foundation(2014M551979) ; Joint Research Laboratory for Sustainable Aviation Biofuels (QIBEBT-Boeing) |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000364452000001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/6776 |
| 专题 | 微生物代谢工程研究组 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Key Lab Biofuels, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Wei,Zhang, Lei,Yao, Lun,et al. Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes[J]. SCIENTIFIC REPORTS,2015,5. |
| APA | Wu, Wei,Zhang, Lei,Yao, Lun,Tan, Xiaoming,Liu, Xufeng,&Lu, Xuefeng.(2015).Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes.SCIENTIFIC REPORTS,5. |
| MLA | Wu, Wei,et al."Genetically assembled fluorescent biosensor for in situ detection of bio-synthesized alkanes".SCIENTIFIC REPORTS 5(2015). |
| 条目包含的文件 | 条目无相关文件。 | |||||
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