Reversible regulation of thrombin adsorption and desorption based on photoresponsive-aptamer modified gold nanoparticles

doi:10.1016/j.talanta.2015.06.053

QIBEBT-IR > 资源环境与绿色分离研究组

	Reversible regulation of thrombin adsorption and desorption based on photoresponsive-aptamer modified gold nanoparticles
	Yu, Jiemiao 1,2,3; Yang, Liangrong 2; Liang, Xiangfeng2 ; Dong, Tingting 2,3; Liu, Huizhou1,2
	2015-11-01
发表期刊	TALANTA
卷号	144 页码:312-317
摘要	In the protein separation, adsorption and desorption of target protein have been using different buffer condition. Different buffer will change the structure and activity of target protein in some cases. This work describes the use of different wavelength light for remote regulation of adsorption and desorption of target protein in the same buffer solutions. A dynamic system that captured and released protein in response to light is reported. Matrix gold nanoparticles and light-responsive affinity ligand comprising thrombin aptamer (APT15), polyethylene glycol linker, and azobenzene-modified complementary sequence were used. UV light induced a trans-cis isomerization of the azobenzene that destabilized the duplex of aptamer and azobenzene-modified complementary sequence, resulting in thrombin binding to aptamer sequence. Visible light irradiation resulted in DNA duplex rehybridization and thrombin released. Our work demonstrates that different light wavelengths effectively regulated the adsorption and desorption of thrombin in the same buffer, and this system also can capture and release prothrombin from plasma with different wavelength light. Furthermore, this method can be widely applied to a variety of different protein separation process. (C) 2015 Elsevier B.V. All rights reserved.
文章类型	Article
关键词	Photoresponsive Thrombin Aptamer Capture Release
WOS标题词	Science & Technology ; Physical Sciences
DOI	10.1016/j.talanta.2015.06.053
关键词[WOS]	AZOBENZENE-TETHERED DNA ; AFFINITY-CHROMATOGRAPHY ; CAPILLARY CHROMATOGRAPHY ; COMBINATORIAL CHEMISTRY ; DUPLEX FORMATION ; PHOTOREGULATION ; TRANSCRIPTION ; POLYMERASE ; SELECTION ; BIND
收录类别	SCI
语种	英语
WOS研究方向	Chemistry
WOS类目	Chemistry, Analytical
WOS记录号	WOS:000363346200044
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6722
专题	资源环境与绿色分离研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Key Lab Green Proc & Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yu, Jiemiao,Yang, Liangrong,Liang, Xiangfeng,et al. Reversible regulation of thrombin adsorption and desorption based on photoresponsive-aptamer modified gold nanoparticles[J]. TALANTA,2015,144:312-317.
APA	Yu, Jiemiao,Yang, Liangrong,Liang, Xiangfeng,Dong, Tingting,&Liu, Huizhou.(2015).Reversible regulation of thrombin adsorption and desorption based on photoresponsive-aptamer modified gold nanoparticles.TALANTA,144,312-317.
MLA	Yu, Jiemiao,et al."Reversible regulation of thrombin adsorption and desorption based on photoresponsive-aptamer modified gold nanoparticles".TALANTA 144(2015):312-317.