QIBEBT-IR  > 生物基材料组群
Enhancement of enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media by ultrasonic intensification
Yang, Fang; Li, Liangzhi; Li, Qiang; Tan, Weigiang; Liu, Wei; Xian, Mo
2010-06-11
发表期刊CARBOHYDRATE POLYMERS
ISSN0144-8617
卷号81期号:2页码:311-316
摘要A new approach for in situ enzymatic saccharification of cellulose in ionic liquids (ILs)-aqueous media is presented in which ultrasonic pretreatment was used to enhance the conversion of cellulose. For this purpose, the solubility of cellulose and the activity of cellulase were investigated in six alkylphosphate ILs. 1-Methyl-3-methylimidazolium dimethylphosphate ([Mmim][DMP]) giving favorable solubility and biocompatibility was selected to establish aqueous-ILs system for enzymatic in situ saccharification of cellulose. After further optimization of reaction parameters concerning cellulase concentration, temperature and IL concentration, higher conversion (95.48%) of cellulose was obtained in the media of aqueous-[Mmim][DMP] by conducting the pretreatment of cellulose with ultrasonic heating, whereas the conversion of cellulose untreated was 42.77%. Scanning electronic microscopy (SEM) and viscosity analysis indicated that IL-treated cellulose under ultrasonic condition was subjected to depolymerizadon, which led to more efficient saccharification. The findings of this study would have great implications for developing a continuous process for transformation of biomass such as straw cellulose to ethanol or other hydrocarbons. (C) 2010 Elsevier Ltd. All rights reserved.; A new approach for in situ enzymatic saccharification of cellulose in ionic liquids (ILs)-aqueous media is presented in which ultrasonic pretreatment was used to enhance the conversion of cellulose. For this purpose, the solubility of cellulose and the activity of cellulase were investigated in six alkylphosphate ILs. 1-Methyl-3-methylimidazolium dimethylphosphate ([Mmim][DMP]) giving favorable solubility and biocompatibility was selected to establish aqueous-ILs system for enzymatic in situ saccharification of cellulose. After further optimization of reaction parameters concerning cellulase concentration, temperature and IL concentration, higher conversion (95.48%) of cellulose was obtained in the media of aqueous-[Mmim][DMP] by conducting the pretreatment of cellulose with ultrasonic heating, whereas the conversion of cellulose untreated was 42.77%. Scanning electronic microscopy (SEM) and viscosity analysis indicated that IL-treated cellulose under ultrasonic condition was subjected to depolymerizadon, which led to more efficient saccharification. The findings of this study would have great implications for developing a continuous process for transformation of biomass such as straw cellulose to ethanol or other hydrocarbons. (C) 2010 Elsevier Ltd. All rights reserved.
文章类型Article
关键词Cellulose Enzymatic Hydrolysis In Situ Saccharification Ionic Liquid Pretreatment Ultrasonic
学科领域生物基化学品
WOS标题词Science & Technology ; Physical Sciences
DOI10.1016/j.carbpol.2010.02.031
关键词[WOS]DISSOLUTION ; HYDROLYSIS ; PRETREATMENT ; BIOCATALYSIS ; MECHANISM ; ENZYMES
收录类别SCI
语种英语
WOS研究方向Chemistry ; Polymer Science
WOS类目Chemistry, Applied ; Chemistry, Organic ; Polymer Science
WOS记录号WOS:000278170400020
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/670
专题生物基材料组群
作者单位Chinese Acad Sci, Key Lab Biofuel, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266061, Peoples R China
推荐引用方式
GB/T 7714
Yang, Fang,Li, Liangzhi,Li, Qiang,et al. Enhancement of enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media by ultrasonic intensification[J]. CARBOHYDRATE POLYMERS,2010,81(2):311-316.
APA Yang, Fang,Li, Liangzhi,Li, Qiang,Tan, Weigiang,Liu, Wei,&Xian, Mo.(2010).Enhancement of enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media by ultrasonic intensification.CARBOHYDRATE POLYMERS,81(2),311-316.
MLA Yang, Fang,et al."Enhancement of enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media by ultrasonic intensification".CARBOHYDRATE POLYMERS 81.2(2010):311-316.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Enhancement of enzym(657KB) 开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yang, Fang]的文章
[Li, Liangzhi]的文章
[Li, Qiang]的文章
百度学术
百度学术中相似的文章
[Yang, Fang]的文章
[Li, Liangzhi]的文章
[Li, Qiang]的文章
必应学术
必应学术中相似的文章
[Yang, Fang]的文章
[Li, Liangzhi]的文章
[Li, Qiang]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Enhancement of enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media by ultrasonic intensification.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。