| Enhancement of enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media by ultrasonic intensification | |
Yang, Fang; Li, Liangzhi; Li, Qiang; Tan, Weigiang; Liu, Wei; Xian, Mo
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| 2010-06-11 | |
| 发表期刊 | CARBOHYDRATE POLYMERS
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| ISSN | 0144-8617 |
| 卷号 | 81期号:2页码:311-316 |
| 摘要 | A new approach for in situ enzymatic saccharification of cellulose in ionic liquids (ILs)-aqueous media is presented in which ultrasonic pretreatment was used to enhance the conversion of cellulose. For this purpose, the solubility of cellulose and the activity of cellulase were investigated in six alkylphosphate ILs. 1-Methyl-3-methylimidazolium dimethylphosphate ([Mmim][DMP]) giving favorable solubility and biocompatibility was selected to establish aqueous-ILs system for enzymatic in situ saccharification of cellulose. After further optimization of reaction parameters concerning cellulase concentration, temperature and IL concentration, higher conversion (95.48%) of cellulose was obtained in the media of aqueous-[Mmim][DMP] by conducting the pretreatment of cellulose with ultrasonic heating, whereas the conversion of cellulose untreated was 42.77%. Scanning electronic microscopy (SEM) and viscosity analysis indicated that IL-treated cellulose under ultrasonic condition was subjected to depolymerizadon, which led to more efficient saccharification. The findings of this study would have great implications for developing a continuous process for transformation of biomass such as straw cellulose to ethanol or other hydrocarbons. (C) 2010 Elsevier Ltd. All rights reserved.; A new approach for in situ enzymatic saccharification of cellulose in ionic liquids (ILs)-aqueous media is presented in which ultrasonic pretreatment was used to enhance the conversion of cellulose. For this purpose, the solubility of cellulose and the activity of cellulase were investigated in six alkylphosphate ILs. 1-Methyl-3-methylimidazolium dimethylphosphate ([Mmim][DMP]) giving favorable solubility and biocompatibility was selected to establish aqueous-ILs system for enzymatic in situ saccharification of cellulose. After further optimization of reaction parameters concerning cellulase concentration, temperature and IL concentration, higher conversion (95.48%) of cellulose was obtained in the media of aqueous-[Mmim][DMP] by conducting the pretreatment of cellulose with ultrasonic heating, whereas the conversion of cellulose untreated was 42.77%. Scanning electronic microscopy (SEM) and viscosity analysis indicated that IL-treated cellulose under ultrasonic condition was subjected to depolymerizadon, which led to more efficient saccharification. The findings of this study would have great implications for developing a continuous process for transformation of biomass such as straw cellulose to ethanol or other hydrocarbons. (C) 2010 Elsevier Ltd. All rights reserved. |
| 文章类型 | Article |
| 关键词 | Cellulose Enzymatic Hydrolysis In Situ Saccharification Ionic Liquid Pretreatment Ultrasonic |
| 学科领域 | 生物基化学品 |
| WOS标题词 | Science & Technology ; Physical Sciences |
| DOI | 10.1016/j.carbpol.2010.02.031 |
| 关键词[WOS] | DISSOLUTION ; HYDROLYSIS ; PRETREATMENT ; BIOCATALYSIS ; MECHANISM ; ENZYMES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Chemistry ; Polymer Science |
| WOS类目 | Chemistry, Applied ; Chemistry, Organic ; Polymer Science |
| WOS记录号 | WOS:000278170400020 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/670 |
| 专题 | 生物基材料组群 |
| 作者单位 | Chinese Acad Sci, Key Lab Biofuel, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266061, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Fang,Li, Liangzhi,Li, Qiang,et al. Enhancement of enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media by ultrasonic intensification[J]. CARBOHYDRATE POLYMERS,2010,81(2):311-316. |
| APA | Yang, Fang,Li, Liangzhi,Li, Qiang,Tan, Weigiang,Liu, Wei,&Xian, Mo.(2010).Enhancement of enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media by ultrasonic intensification.CARBOHYDRATE POLYMERS,81(2),311-316. |
| MLA | Yang, Fang,et al."Enhancement of enzymatic in situ saccharification of cellulose in aqueous-ionic liquid media by ultrasonic intensification".CARBOHYDRATE POLYMERS 81.2(2010):311-316. |
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