Simultaneous accumulation of neutral lipids and biomass in Nannochloropsis oceanica IMET1 under high light intensity and nitrogen replete conditions

doi:10.1016/j.algal.2015.05.019

QIBEBT-IR > 代谢物组学研究组

	Simultaneous accumulation of neutral lipids and biomass in Nannochloropsis oceanica IMET1 under high light intensity and nitrogen replete conditions
	Xiao, Yan1,2 ; Zhang, Jingtao 1,2; Cui, Jiatao 1,2; Yao, Xingzhe 1,2,4; Sun, Zhijie1,2 ; Feng, Yingang1,2,3 ; Cui, Qiu1,2,3
	2015-09-01
发表期刊	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷号	11 页码:55-62
摘要	The difficulty in simultaneously obtaining a high production of neutral lipids and biomass in the oleaginous microalga Nannochloropsis is an attractive challenge that needs to be addressed. To overcome this challenge and unveil the underlying metabolic characteristics, the model oleaginous microalga Nannochloropsis oceanica IMET1, under high light intensity and nitrogen-replete conditions, was prepared via steady-state continuous culture in the present study. The simultaneous accumulation of lipids, particularly neutral lipids, and biomass in N. oceanica IMET1 was triggered at a high light intensity (331.2 mu mol photons m(-2) s(-1)) in the light saturation region accompanied by a sufficient nitrogen supply (2000 mu mol L-1). Moreover, high amounts of valuable fatty acids (arachidonic acid and eicosapentaenoic acid) were also produced. The distribution of fatty acids and intracellular soluble metabolites, the membrane lipid composition and the photosynthetic pathway were associated with the concurrent accumulation of biomass and TAGs in N. oceanica IMET1. The common and different metabolic responses between high-light and nitrogen-depletion strategies for the accumulation of neutral lipids were also discussed. These results provide detailed metabolic clues for the genetic and metabolic engineering of Nannochloropsis for biofuel production. (C) 2015 Elsevier B.V. All rights reserved.
文章类型	Article
关键词	Nannochloropsis Neutral Lipid Biodiesel High Light Intensity Nitrogen Repletion
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.algal.2015.05.019
关键词[WOS]	GENOME SEQUENCE ; FATTY-ACIDS ; MICROALGAE ; BIODIESEL ; OIL ; TRIACYLGLYCEROL ; STARVATION ; GROWTH ; EUSTIGMATOPHYCEAE ; PHOTOSYNTHESIS
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000363046900008
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6697
专题	代谢物组学研究组
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Lab Single Cell Oil, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 4.Chinese Acad Sci, Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Xiao, Yan,Zhang, Jingtao,Cui, Jiatao,et al. Simultaneous accumulation of neutral lipids and biomass in Nannochloropsis oceanica IMET1 under high light intensity and nitrogen replete conditions[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2015,11:55-62.
APA	Xiao, Yan.,Zhang, Jingtao.,Cui, Jiatao.,Yao, Xingzhe.,Sun, Zhijie.,...&Cui, Qiu.(2015).Simultaneous accumulation of neutral lipids and biomass in Nannochloropsis oceanica IMET1 under high light intensity and nitrogen replete conditions.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,11,55-62.
MLA	Xiao, Yan,et al."Simultaneous accumulation of neutral lipids and biomass in Nannochloropsis oceanica IMET1 under high light intensity and nitrogen replete conditions".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 11(2015):55-62.