The effect of laccase on cellulase-treated lignin in 1-n-butyl-3-methylimidazolium chloride/H2O homogeneous and H2O heterogeneous solutions

doi:10.5897/AJB11.1607

QIBEBT-IR > 生物基材料组群

	The effect of laccase on cellulase-treated lignin in 1-n-butyl-3-methylimidazolium chloride/H2O homogeneous and H2O heterogeneous solutions
	Zhang Yinglong 1,2; Zhang Haibo1,3 ; Zhu Youshuang 1; Huang Feng 1
	2011-11-07
发表期刊	AFRICAN JOURNAL OF BIOTECHNOLOGY
卷号	10 期号:69 页码:15647-15655
摘要	In this study, the effect of laccase earlier obtained by our laboratory on cellulase-treated lignin (CEL) in two different solution systems was further investigated. Results obtained were as follows: After laccase treatment of CEL in the heterogeneous water solution, CEL was then compared with control sample A. Ultraviolet (UV) spectra showed that the total absorbance of sample B increased at 205 and 280 nm; Fourier-transform infrared (FTIR) spectra showed that the carbonyl group obviously appeared; and C-13 nuclear magnetic resonance (NMR) spectra showed that the C-gamma signals disappeared, the C-alpha signals significantly increased, and the methoxyl content decreased. After laccase treatment of CEL in the [bmim]Cl/H2O homogeneous solution, CEL was compared with control sample C. UV spectra showed that the total absorbance of sample D decreased at 280 nm; high-performance liquid chromatography (HPLC) analysis showed that some small absorption peaks disappeared; and C-13 NMR spectra showed that the C-gamma signals obviously increased, the C-alpha signals slightly decreased, while the methoxyl content increased. Clearly, for insoluble CEL in the heterogeneous water solution, laccase partly oxidized the hydroxyl group into a carbonyl group, and thereby partly degraded CEL to increase its solubility. In contrast, for soluble CEL in the [bmim]Cl/H2O homogeneous solution, laccase primarily polymerized small molecule fragments with the CEL macromolecule, increased the methoxyl content of CEL, and thereby played a role in lignin polymerization.
文章类型	Article
关键词	Cellulase-treated Lignin Laccase [Bmim]Cl
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.5897/AJB11.1607
关键词[WOS]	IONIC LIQUIDS ; ENZYME ; SYSTEM ; STABILITY ; SOLVENTS
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000298555400025
引用统计	被引频次：1[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6639
专题	生物基材料组群
作者单位	1.Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China 2.Shandong Inst Commerce & Technol, Dept Biotechnol, Jinan 250103, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Zhang Yinglong,Zhang Haibo,Zhu Youshuang,et al. The effect of laccase on cellulase-treated lignin in 1-n-butyl-3-methylimidazolium chloride/H2O homogeneous and H2O heterogeneous solutions[J]. AFRICAN JOURNAL OF BIOTECHNOLOGY,2011,10(69):15647-15655.
APA	Zhang Yinglong,Zhang Haibo,Zhu Youshuang,&Huang Feng.(2011).The effect of laccase on cellulase-treated lignin in 1-n-butyl-3-methylimidazolium chloride/H2O homogeneous and H2O heterogeneous solutions.AFRICAN JOURNAL OF BIOTECHNOLOGY,10(69),15647-15655.
MLA	Zhang Yinglong,et al."The effect of laccase on cellulase-treated lignin in 1-n-butyl-3-methylimidazolium chloride/H2O homogeneous and H2O heterogeneous solutions".AFRICAN JOURNAL OF BIOTECHNOLOGY 10.69(2011):15647-15655.