KMS Qingdao Institute of Biomass Energy and Bioprocess Technology ,CAS
The pH Shift and Precursor Feeding Strategy in a Low-Toxicity FR-008/Candicidin Derivative CS103 Fermentation Bioprocess by a Mutant of Streptomyces sp FR-008 | |
Mao, Xiangzhao1,2; Wang, Feng1; Zhang, Jianguo1; Chen, Shi3,4; Deng, Zixin3,4; Shen, Yaling1; Wei, Dongzhi1,2 | |
2009-12-01 | |
发表期刊 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY |
卷号 | 159期号:3页码:673-686 |
摘要 | CS103, the novel derivative of polyene macrolides antibiotic FR-008/candicidin with lower toxicity has been isolated from the culture mycelia of the mutant of Streptomyces sp. FR-008, with targeted deletions of the fscP cytochrome P450 gene from its chromosome. To enhance biosynthesis of CS103, pH shift and precursor feeding strategy for fermentation process by the mutant of Streptomyces sp. FR-008 in a stirred tank bioreactor was developed. According to the process parameters analysis, the effectiveness of the strategy was examined and confirmed by experiments. A maximal CS103 concentration of 139.98 mu g/mL was obtained, 2.05-fold higher than that in the pH-uncontrolled fermentation. Compared to other three cases as pH-uncontrolled, pH-controlled, and two-stage pH-controlled batch cultures, the proposed "pH shift and precursor feeding strategy" effectively avoided the scarcity of the antibiotic precursor, increased the CS103 yield from biomass (Y (P/X)) and substrate (Y (P/S)) by 110.61% and 48.52%, respectively, and at the time the fermentation time was shortened from 120 to 96 h. The highest CS103 production rate (1.46 mu g mL(-1) h(-1)) of the pH shift and precursor feeding strategy was 284.21%, 97.30%, and 58.70% higher than that of pH-uncontrolled, pH-controlled, and two-stage pH-controlled batch culture cases, respectively. |
文章类型 | Article |
关键词 | Two-stage Ph Control Strategy Precursor Feeding Polyene Macrolides Antibiotic Low-toxicity Fr-008/candicidin Fermentation |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
DOI | 10.1007/s12010-008-8502-y |
关键词[WOS] | MACROLIDE ANTIBIOTICS ; POLYENE MACROLIDES ; CANDICIDIN ; CARBOXYL |
收录类别 | SCI ; ISTP |
语种 | 英语 |
WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
WOS类目 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology |
WOS记录号 | WOS:000271068000010 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qibebt.ac.cn/handle/337004/6633 |
专题 | 生物反应工程团队(过去) |
作者单位 | 1.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266071, Peoples R China 3.Shanghai Jiao Tong Univ, Lab Microbial Metab, Shanghai 200030, Peoples R China 4.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200030, Peoples R China |
推荐引用方式 GB/T 7714 | Mao, Xiangzhao,Wang, Feng,Zhang, Jianguo,et al. The pH Shift and Precursor Feeding Strategy in a Low-Toxicity FR-008/Candicidin Derivative CS103 Fermentation Bioprocess by a Mutant of Streptomyces sp FR-008[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2009,159(3):673-686. |
APA | Mao, Xiangzhao.,Wang, Feng.,Zhang, Jianguo.,Chen, Shi.,Deng, Zixin.,...&Wei, Dongzhi.(2009).The pH Shift and Precursor Feeding Strategy in a Low-Toxicity FR-008/Candicidin Derivative CS103 Fermentation Bioprocess by a Mutant of Streptomyces sp FR-008.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,159(3),673-686. |
MLA | Mao, Xiangzhao,et al."The pH Shift and Precursor Feeding Strategy in a Low-Toxicity FR-008/Candicidin Derivative CS103 Fermentation Bioprocess by a Mutant of Streptomyces sp FR-008".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 159.3(2009):673-686. |
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