Succinate Production from Sucrose by Metabolic Engineered Escherichia coli Strains Under Aerobic Conditions

doi:10.1002/btpr.661

QIBEBT-IR > 微生物代谢工程研究组

	Succinate Production from Sucrose by Metabolic Engineered Escherichia coli Strains Under Aerobic Conditions
	Wang, Jian 5; Zhu, Jiangfeng 4; Bennett, George N.3; San, Ka-Yiu 1,2
	2011-09-01
发表期刊	BIOTECHNOLOGY PROGRESS
卷号	27 期号:5 页码:1242-1247
摘要	Two metabolically engineered E. coli strains HL2765k and HL27659k, while capable of producing succinate from glucose with high yields, are not able to grow and produce succinate on sucrose. Consequently, the pUR400 plasmid containing scrK, Y, A, B, and R genes was introduced into HL2765k and HL27659k, respectively. Shake flask culture studies showed that the resulting strains can utilize sucrose; the strain HL2765k pUR400 and HL27659k pUR400 can produce succinate aerobically with a molar yield of 0.78 +/- 0.02 mol/mol and 1.35 +/- 0.13 mol/mol, respectively. On introduction of the plasmid pHL413, which encodes the heterologous pyruvate carboxylase (PYC) from Lactococcus lactis, the molar succinate yield increased to 1.60 +/- 0.01 mol of succinate per mole of sucrose by the HL2765k pUR400 pHL413 strain and to 1.84 +/- 0.10 by the HL27659k pUR400 pHL413 strain. In aerobic batch bioreactor studies, the succinate production rate was faster, and succinate production reached 101.83 mM with a yield of 1.90 when dissolved oxygen (DO) was controlled at 40 +/- 7%. In addition, the results showed that DO had an important effect on succinate production by influencing PYC activity. This work demonstrates the possibility of producing succinate aerobically using sucrose as the carbon source. (C) 2011 American Institute of Chemical Engineers Biotechnol. Prog., 27: 1242-1247, 2011
文章类型	Article
关键词	Escherichia Coli Fermentation Sucrose Aerobic Succinate Production
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1002/btpr.661
关键词[WOS]	PYRUVATE-CARBOXYLASE ; PHOSPHOENOLPYRUVATE CARBOXYLASE ; ACID PRODUCTION ; MUTANT STRAINS ; OVEREXPRESSION ; PATHWAYS ; PLASMID ; FERMENTATION ; EVOLUTION ; INDUSTRY
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology ; Food Science & Technology
WOS类目	Biotechnology & Applied Microbiology ; Food Science & Technology
WOS记录号	WOS:000296061900006
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6628
专题	微生物代谢工程研究组
作者单位	1.Rice Univ, Dept Bioengn, Houston, TX 77030 USA 2.Rice Univ, Dept Chem & Biomol Engn, Houston, TX 77251 USA 3.Rice Univ, Dept Biochem & Cell Biol, Houston, TX 77251 USA 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Peoples R China 5.Jilin Univ, Coll Agr & Biol Engn, Changchun 130023, Peoples R China
推荐引用方式 GB/T 7714	Wang, Jian,Zhu, Jiangfeng,Bennett, George N.,et al. Succinate Production from Sucrose by Metabolic Engineered Escherichia coli Strains Under Aerobic Conditions[J]. BIOTECHNOLOGY PROGRESS,2011,27(5):1242-1247.
APA	Wang, Jian,Zhu, Jiangfeng,Bennett, George N.,&San, Ka-Yiu.(2011).Succinate Production from Sucrose by Metabolic Engineered Escherichia coli Strains Under Aerobic Conditions.BIOTECHNOLOGY PROGRESS,27(5),1242-1247.
MLA	Wang, Jian,et al."Succinate Production from Sucrose by Metabolic Engineered Escherichia coli Strains Under Aerobic Conditions".BIOTECHNOLOGY PROGRESS 27.5(2011):1242-1247.