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Bioproduction of Glycolic Acid from Glycolonitrile with a New Bacterial Isolate of Alcaligenes sp ECU0401
He, Yu-Cai1,2; Xu, Jian-He1; Su, Jin-Huan1; Zhou, Li1
2010-03-01
发表期刊APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
卷号160期号:5页码:1428-1440
摘要

Alcaligenes sp. ECU0401 has been isolated from soil samples with high nitrilase activity against glycolonitrile using the enrichment culture technique. The preferred carbon/nitrogen sources and metal ions were sodium acetate, a composite of peptone and yeast extract, and Cu(2+), respectively. Glycolic acid was obtained in a yield of 96.5% after 14 h of biotransformation from a total of 200 mM glycolonitrile in the mode of sequential addition during the cultivation of Alcaligenes sp. ECU0401 in a 5-L jar fermenter. Fifty micromolars of glycolonitrile could be hydrolyzed in a yield of 94.1% by resting cells after 36 h. The microbial nitrilase system could hydrolyze various nitriles with high activity, and no amidase activity and glycolic acid were observed in hydrolyzing glycolamide. It significantly exhibited high enantioselectivity in the hydrolysis of mandelonitrile and 2-chloromandelonitrile (> 99.9% e.e. (p) ). Efficient biocatalyst recycling was achieved as a result of immobilization in glutaraldehyde/polyethylenimine cross-linked carrageenan with immobilized cells exhibiting a biocatalyst productivity of 1,042.2 g glycolic acid per gram dry cell weight after 29 batch recycles.

文章类型Article
关键词Nitrilase Alcaligenes Sp Ecu0401 Glycolic Acid Glycolonitrile Immobilization
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s12010-009-8607-y
关键词[WOS]RHODOCOCCUS-RHODOCHROUS J1 ; (R)-(-)-MANDELIC ACID ; PSEUDOMONAS-PUTIDA ; NITRILASE ; MANDELONITRILE ; BIOSYNTHESIS ; OPTIMIZATION ; PURIFICATION ; CLONING ; GENE
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS类目Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
WOS记录号WOS:000274398500016
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/6581
专题生物基材料组群
作者单位1.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biocatalysis & Bioproc, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao 266071, Peoples R China
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He, Yu-Cai,Xu, Jian-He,Su, Jin-Huan,et al. Bioproduction of Glycolic Acid from Glycolonitrile with a New Bacterial Isolate of Alcaligenes sp ECU0401[J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,2010,160(5):1428-1440.
APA He, Yu-Cai,Xu, Jian-He,Su, Jin-Huan,&Zhou, Li.(2010).Bioproduction of Glycolic Acid from Glycolonitrile with a New Bacterial Isolate of Alcaligenes sp ECU0401.APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY,160(5),1428-1440.
MLA He, Yu-Cai,et al."Bioproduction of Glycolic Acid from Glycolonitrile with a New Bacterial Isolate of Alcaligenes sp ECU0401".APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 160.5(2010):1428-1440.
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