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Effect of culture operating conditions on succinate production in a multiphase fed-batch bioreactor using an engineered Escherichia coli strain
Zhu, Jiangfeng4,5; Thakker, Chandresh1; San, Ka-Yiu2,3; Bennett, George1
2011-11-01
发表期刊APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
卷号92期号:3页码:499-508
摘要

A metabolically engineered Escherichia coli strain SBS550MG (pHL413) was used in this study to investigate the impact of various culture operating conditions for improving the specific succinate production rate for better final titer while maintaining the theoretical succinate yield on glucose in multiphase fed-batch cultures. Previously, we reported that changes in the level of aeration during the cell growth phase significantly modified gene expression profiles and metabolic fluxes in this system (Martinez et al. 2010). Based on these observations, the examination of culture conditions was mainly focused on the aerobic growth phase. It was found that 2-5 h of low dissolved oxygen culture during the aerobic phase improves cell productivity, but pH control during the aerobic phase was not favorable for the system. Cell viability has been identified as a major limiting factor for succinate production. Supplementing LB medium and betaine, an anti-osmotic stress reagent, did not improve cell activity. A higher succinate titer (537.8 mM) using the current metabolic engineering E. coli strain was achieved, which can potentially be improved further by increasing cell viability.

文章类型Article
关键词Succinate Production Escherichia Coli Culture Conditions Multiphase Fermentation
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1007/s00253-011-3314-3
关键词[WOS]ACID PRODUCTION ; ANAEROBIOSPIRILLUM-SUCCINICIPRODUCENS ; MANNHEIMIA-SUCCINICIPRODUCENS ; VOLUMETRIC PRODUCTIVITY ; METABOLIC FLUXES ; PROTEIN ; FERMENTATION ; EXPRESSION ; PHOSPHORYLATION ; REDISTRIBUTION
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000295673800007
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/6574
专题微生物代谢工程研究组
作者单位1.Rice Univ, Dept Biochem & Cell Biol, Houston, TX 77005 USA
2.Rice Univ, Dept Bioengn, Houston, TX 77005 USA
3.Rice Univ, Dept Chem & Biomol Engn, Houston, TX 77005 USA
4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Peoples R China
5.Chinese Acad Sci, Key Lab Biofuels, Qingdao, Peoples R China
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Zhu, Jiangfeng,Thakker, Chandresh,San, Ka-Yiu,et al. Effect of culture operating conditions on succinate production in a multiphase fed-batch bioreactor using an engineered Escherichia coli strain[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2011,92(3):499-508.
APA Zhu, Jiangfeng,Thakker, Chandresh,San, Ka-Yiu,&Bennett, George.(2011).Effect of culture operating conditions on succinate production in a multiphase fed-batch bioreactor using an engineered Escherichia coli strain.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,92(3),499-508.
MLA Zhu, Jiangfeng,et al."Effect of culture operating conditions on succinate production in a multiphase fed-batch bioreactor using an engineered Escherichia coli strain".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 92.3(2011):499-508.
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