Effect of culture operating conditions on succinate production in a multiphase fed-batch bioreactor using an engineered Escherichia coli strain | |
Zhu, Jiangfeng4,5; Thakker, Chandresh1; San, Ka-Yiu2,3; Bennett, George1 | |
2011-11-01 | |
发表期刊 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY |
卷号 | 92期号:3页码:499-508 |
摘要 | A metabolically engineered Escherichia coli strain SBS550MG (pHL413) was used in this study to investigate the impact of various culture operating conditions for improving the specific succinate production rate for better final titer while maintaining the theoretical succinate yield on glucose in multiphase fed-batch cultures. Previously, we reported that changes in the level of aeration during the cell growth phase significantly modified gene expression profiles and metabolic fluxes in this system (Martinez et al. 2010). Based on these observations, the examination of culture conditions was mainly focused on the aerobic growth phase. It was found that 2-5 h of low dissolved oxygen culture during the aerobic phase improves cell productivity, but pH control during the aerobic phase was not favorable for the system. Cell viability has been identified as a major limiting factor for succinate production. Supplementing LB medium and betaine, an anti-osmotic stress reagent, did not improve cell activity. A higher succinate titer (537.8 mM) using the current metabolic engineering E. coli strain was achieved, which can potentially be improved further by increasing cell viability. |
文章类型 | Article |
关键词 | Succinate Production Escherichia Coli Culture Conditions Multiphase Fermentation |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
DOI | 10.1007/s00253-011-3314-3 |
关键词[WOS] | ACID PRODUCTION ; ANAEROBIOSPIRILLUM-SUCCINICIPRODUCENS ; MANNHEIMIA-SUCCINICIPRODUCENS ; VOLUMETRIC PRODUCTIVITY ; METABOLIC FLUXES ; PROTEIN ; FERMENTATION ; EXPRESSION ; PHOSPHORYLATION ; REDISTRIBUTION |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Biotechnology & Applied Microbiology |
WOS类目 | Biotechnology & Applied Microbiology |
WOS记录号 | WOS:000295673800007 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qibebt.ac.cn/handle/337004/6574 |
专题 | 微生物代谢工程研究组 |
作者单位 | 1.Rice Univ, Dept Biochem & Cell Biol, Houston, TX 77005 USA 2.Rice Univ, Dept Bioengn, Houston, TX 77005 USA 3.Rice Univ, Dept Chem & Biomol Engn, Houston, TX 77005 USA 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Peoples R China 5.Chinese Acad Sci, Key Lab Biofuels, Qingdao, Peoples R China |
推荐引用方式 GB/T 7714 | Zhu, Jiangfeng,Thakker, Chandresh,San, Ka-Yiu,et al. Effect of culture operating conditions on succinate production in a multiphase fed-batch bioreactor using an engineered Escherichia coli strain[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2011,92(3):499-508. |
APA | Zhu, Jiangfeng,Thakker, Chandresh,San, Ka-Yiu,&Bennett, George.(2011).Effect of culture operating conditions on succinate production in a multiphase fed-batch bioreactor using an engineered Escherichia coli strain.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,92(3),499-508. |
MLA | Zhu, Jiangfeng,et al."Effect of culture operating conditions on succinate production in a multiphase fed-batch bioreactor using an engineered Escherichia coli strain".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 92.3(2011):499-508. |
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