| Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere | |
| Gu, Likun1,2; Bai, Zhihui1; Jin, Bo1,3; Hu, Qing1; Wang, Huili1; Zhuang, Guoqiang1; Zhang, Hongxun2,4 | |
| 2010 | |
| 发表期刊 | JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
 |
| 卷号 | 22期号:1页码:134-141 |
| 摘要 | Fungicides have been used extensively for controlling fungal pathogens of plants. However, little is known regarding the effects that fungicides upon the indigenous bacterial communities within the plant phyllosphere. The aims of this study were to assess the impact of fungicide enostroburin upon bacterial communities in wheat phyllosphere. Culture-independent methodologies of 16S rDNA clone library and 16S rDNA directed polymerase chain reaction with denaturing gradient gel electrophoresis (PCR-DGGE) were used for monitoring the change of bacterial community. The 16S rDNA clone library and PCR-DGGE analysis both confirmed the microbial community of wheat plant phyllosphere were predominantly of the gamma-Proteobacteria phyla. Results from PCR-DGGE analysis indicated a significant change in bacterial community structure within the phyllosphere following fungicide enostroburin application. Bands sequenced within control cultures were predominantly of Pseudomonas genus, but those bands sequenced in the treated samples were predominantly strains of Pantoea genus and Pseudomonas genus. Of interest was the appearance of two DGGE bands following fungicide treatment, one of which had sequence similarities (98%) to Pantoea sp. which might be a competitor of plant pathogens. This study revealed the wheat phyllosphere bacterial community composition and a shift in the bacterial community following fungicide enostroburin application. |
| 文章类型 | Article |
| 关键词 | Fungicide Phyllosphere Microbial Community 16s Rdna Clone Library Pcr-dgge |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1016/S1001-0742(09)60084-X |
| 关键词[WOS] | GROWTH-PROMOTING BACTERIA ; MICROBIAL COMMUNITY ; EPIPHYTIC FITNESS ; ERWINIA-HERBICOLA ; DIVERSITY ; SOILS ; LEAF ; POPULATIONS ; DNA ; MICROORGANISMS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Environmental Sciences & Ecology |
| WOS类目 | Environmental Sciences |
| WOS记录号 | WOS:000273860800018 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/6570 |
| 专题 | 蛋白质设计研究组 |
| 作者单位 | 1.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China 2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao 266101, Peoples R China 3.Univ Adelaide, Sch Earth & Environm Sci, Adelaide, SA 5005, Australia 4.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gu, Likun,Bai, Zhihui,Jin, Bo,et al. Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2010,22(1):134-141. |
| APA | Gu, Likun.,Bai, Zhihui.,Jin, Bo.,Hu, Qing.,Wang, Huili.,...&Zhang, Hongxun.(2010).Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,22(1),134-141. |
| MLA | Gu, Likun,et al."Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 22.1(2010):134-141. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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