Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere

doi:10.1016/S1001-0742(09)60084-X

QIBEBT-IR > 蛋白质设计研究组

	Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere
	Gu, Likun 1,2; Bai, Zhihui 1; Jin, Bo 1,3; Hu, Qing 1; Wang, Huili 1; Zhuang, Guoqiang 1; Zhang, Hongxun 2,4
	2010
发表期刊	JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
卷号	22 期号:1 页码:134-141
摘要	Fungicides have been used extensively for controlling fungal pathogens of plants. However, little is known regarding the effects that fungicides upon the indigenous bacterial communities within the plant phyllosphere. The aims of this study were to assess the impact of fungicide enostroburin upon bacterial communities in wheat phyllosphere. Culture-independent methodologies of 16S rDNA clone library and 16S rDNA directed polymerase chain reaction with denaturing gradient gel electrophoresis (PCR-DGGE) were used for monitoring the change of bacterial community. The 16S rDNA clone library and PCR-DGGE analysis both confirmed the microbial community of wheat plant phyllosphere were predominantly of the gamma-Proteobacteria phyla. Results from PCR-DGGE analysis indicated a significant change in bacterial community structure within the phyllosphere following fungicide enostroburin application. Bands sequenced within control cultures were predominantly of Pseudomonas genus, but those bands sequenced in the treated samples were predominantly strains of Pantoea genus and Pseudomonas genus. Of interest was the appearance of two DGGE bands following fungicide treatment, one of which had sequence similarities (98%) to Pantoea sp. which might be a competitor of plant pathogens. This study revealed the wheat phyllosphere bacterial community composition and a shift in the bacterial community following fungicide enostroburin application.
文章类型	Article
关键词	Fungicide Phyllosphere Microbial Community 16s Rdna Clone Library Pcr-dgge
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/S1001-0742(09)60084-X
关键词[WOS]	GROWTH-PROMOTING BACTERIA ; MICROBIAL COMMUNITY ; EPIPHYTIC FITNESS ; ERWINIA-HERBICOLA ; DIVERSITY ; SOILS ; LEAF ; POPULATIONS ; DNA ; MICROORGANISMS
收录类别	SCI
语种	英语
WOS研究方向	Environmental Sciences & Ecology
WOS类目	Environmental Sciences
WOS记录号	WOS:000273860800018
引用统计	被引频次：42[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6570
专题	蛋白质设计研究组
作者单位	1.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China 2.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao 266101, Peoples R China 3.Univ Adelaide, Sch Earth & Environm Sci, Adelaide, SA 5005, Australia 4.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Gu, Likun,Bai, Zhihui,Jin, Bo,et al. Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2010,22(1):134-141.
APA	Gu, Likun.,Bai, Zhihui.,Jin, Bo.,Hu, Qing.,Wang, Huili.,...&Zhang, Hongxun.(2010).Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,22(1),134-141.
MLA	Gu, Likun,et al."Assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 22.1(2010):134-141.