An Easy and Efficient Fluorescent Method for Detecting Aldehydes and Its Application in Biotransformation

doi:10.1007/s10895-010-0746-6

	An Easy and Efficient Fluorescent Method for Detecting Aldehydes and Its Application in Biotransformation
	Xing, Yanlong2 ; Wang, Shu 2; Mao, Xiangzhao 2; Zhao, Xuebo 2; Wei, Dongzhi 1
	2011-03-01
发表期刊	JOURNAL OF FLUORESCENCE
卷号	21 期号:2 页码:587-594
摘要	Water-soluble aldehydes (acetaldehyde, propionaldehyde) and non-water-soluble aldehydes (butyraldehyde and phenylacetaldehyde) were easily detected by an efficient fluorescent method with 5-aminofluorescein as probe. Under optimal detection conditions, 5-aminofluorescein could selectively respond to aldehydes with high sensitivity in comparison with other carbonyl compounds like ketones and acids. Thus, the proposed method was used to monitor microbial oxidation and succeeded in trapping transiently-produced aldehydes during biotransformation of primary alcohols by Gluconobacter oxydans.
文章类型	Article
关键词	Fluorescent Probe 5-aminofluorescein Aldehydes Microbial Oxidation
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1007/s10895-010-0746-6
关键词[WOS]	PERFORMANCE LIQUID-CHROMATOGRAPHY ; CARBONYL-COMPOUNDS ; GLUCONOBACTER-OXYDANS ; ALIPHATIC-ALDEHYDES ; CARBOXYLIC-ACIDS ; XANTHENE DYES ; OXIDATION ; AGGREGATION ; HYDRAZINE ; REAGENTS
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemical Research Methods ; Chemistry, Analytical
WOS记录号	WOS:000291061500013
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6560
专题	储氢及新型纳孔材料团队（过去）
作者单位	1.E China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Xing, Yanlong,Wang, Shu,Mao, Xiangzhao,et al. An Easy and Efficient Fluorescent Method for Detecting Aldehydes and Its Application in Biotransformation[J]. JOURNAL OF FLUORESCENCE,2011,21(2):587-594.
APA	Xing, Yanlong,Wang, Shu,Mao, Xiangzhao,Zhao, Xuebo,&Wei, Dongzhi.(2011).An Easy and Efficient Fluorescent Method for Detecting Aldehydes and Its Application in Biotransformation.JOURNAL OF FLUORESCENCE,21(2),587-594.
MLA	Xing, Yanlong,et al."An Easy and Efficient Fluorescent Method for Detecting Aldehydes and Its Application in Biotransformation".JOURNAL OF FLUORESCENCE 21.2(2011):587-594.