Rapid Salt-Extraction of Genomic DNA from Formalin-Fixed Toad and Frog Tissues for PCR-Based Analyses

doi:10.3923/ajava.2011.958.965

QIBEBT-IR > 仿生与固态能源系统研究组

	Rapid Salt-Extraction of Genomic DNA from Formalin-Fixed Toad and Frog Tissues for PCR-Based Analyses
	Liu, Wu-Yi 1,2; Zhang, Ke-Jun 3
	2011-09-01
发表期刊	ASIAN JOURNAL OF ANIMAL AND VETERINARY ADVANCES
卷号	6 期号:9 页码:958-965
摘要	The technical procedures for extraction of DNA from formalin-fixed tissues include many steps such as chemical treatment, enzymatic digestion, phenol-chloroform purification and alcohol precipitation. Formalin-fixed specimens used in molecular cell and DNA studies have shown shortcomings with respect to the efficacy of DNA isolation and subsequent PCR (Polymerase Chain Reaction) amplification. This study was designed to simplify and maximize recovery of PCR-amplifiable DNA from formalin-fixed toad and frog specimens and also to minimize co-extraction of substances that inhibit PCR amplification. This is achieved by a combination of DNA extraction from formalin-fixed muscle tissues using a salt-out buffer consisting of EDTA and proteinase K and NaCl. All steps are performed at room temperature (20-25 degrees C), thereby reducing further degradation of the already damaged fragile specimen DNA and providing an optimal trade-off between DNA release and degradation. The salt-extraction method of genomic DNA presented here allows DNA isolation from formalin-fixed tissues with a minimum of working steps and equipment and rapidly yields much DNA.
文章类型	Article
关键词	Toad And Frog Salt-extraction Genomic Dna Formalin Pcr Amplification
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.3923/ajava.2011.958.965
关键词[WOS]	PARAFFIN-EMBEDDED TISSUES ; POLYMERASE-CHAIN-REACTION ; GENE-EXPRESSION ; REACTION AMPLIFICATION ; BUCCAL CELLS ; SECTIONS ; RETRIEVAL ; SAMPLES ; OPTIMIZATION ; COLLECTION
收录类别	SCI
语种	英语
WOS研究方向	Veterinary Sciences
WOS类目	Veterinary Sciences
WOS记录号	WOS:000294653200009
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6549
专题	仿生与固态能源系统研究组
作者单位	1.Fuyang Normal Coll, Dept Biol Sci, Fuyang City 236041, Peoples R China 2.Fuyang Normal Coll, Dept Sci Res, Fuyang City 236041, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Biomass Energy & Bioproc Technol, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Liu, Wu-Yi,Zhang, Ke-Jun. Rapid Salt-Extraction of Genomic DNA from Formalin-Fixed Toad and Frog Tissues for PCR-Based Analyses[J]. ASIAN JOURNAL OF ANIMAL AND VETERINARY ADVANCES,2011,6(9):958-965.
APA	Liu, Wu-Yi,&Zhang, Ke-Jun.(2011).Rapid Salt-Extraction of Genomic DNA from Formalin-Fixed Toad and Frog Tissues for PCR-Based Analyses.ASIAN JOURNAL OF ANIMAL AND VETERINARY ADVANCES,6(9),958-965.
MLA	Liu, Wu-Yi,et al."Rapid Salt-Extraction of Genomic DNA from Formalin-Fixed Toad and Frog Tissues for PCR-Based Analyses".ASIAN JOURNAL OF ANIMAL AND VETERINARY ADVANCES 6.9(2011):958-965.