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Autotrophic growth of nitrifying community in an agricultural soil
Xia, Weiwei1,2; Zhang, Caixia3; Zeng, Xiaowei4; Feng, Youzhi1; Weng, Jiahua1; Lin, Xiangui1; Zhu, Jianguo1; Xiong, Zhengqin3; Xu, Jian4; Cai, Zucong1; Jia, Zhongjun1
2011-07-01
Source PublicationISME JOURNAL
Volume5Issue:7Pages:1226-1236
AbstractThe two-step nitrification process is an integral part of the global nitrogen cycle, and it is accomplished by distinctly different nitrifiers. By combining DNA-based stable isotope probing (SIP) and high-throughput pyrosequencing, we present the molecular evidence for autotrophic growth of ammonia-oxidizing bacteria (AOB), ammonia-oxidizing archaea (AOA) and nitrite-oxidizing bacteria (NOB) in agricultural soil upon ammonium fertilization. Time-course incubation of SIP microcosms indicated that the amoA genes of AOB was increasingly labeled by (13)CO(2) after incubation for 3, 7 and 28 days during active nitrification, whereas labeling of the AOA amoA gene was detected to a much lesser extent only after a 28-day incubation. Phylogenetic analysis of the (13)C-labeled amoA and 16S rRNA genes revealed that the Nitrosospira cluster 3-like sequences dominate the active AOB community and that active AOA is affiliated with the moderately thermophilic Nitrososphaera gargensis from a hot spring. The higher relative frequency of Nitrospira-like NOB in the (13)C-labeled DNA suggests that it may be more actively involved in nitrite oxidation than Nitrobacter-like NOB. Furthermore, the acetylene inhibition technique showed that (13)CO(2) assimilation by AOB, AOA and NOB occurs only when ammonia oxidation is not blocked, which provides strong hints for the chemolithoautotrophy of nitrifying community in complex soil environments. These results show that the microbial community of AOB and NOB dominates the nitrification process in the agricultural soil tested. The ISME Journal (2011) 5, 1226-1236; doi:10.1038/ismej.2011.5; published online 17 February 2011 Subject Category: microbial ecology and functional diversity of natural habitats
SubtypeArticle
KeywordStable Isotope Probing Pyrosequencing Ammonia-oxidizing Archaea Ammonia-oxidizing Bacteria Nitrite-oxidizing Bacteria Agricultural Soil
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1038/ismej.2011.5
WOS KeywordAMMONIA-OXIDIZING ARCHAEA ; LONG-TERM APPLICATION ; SANDY LOAM SOIL ; IN-SITU ; RIBOSOMAL-RNA ; FRESH-WATER ; MESOPHILIC CRENARCHAEOTA ; NITRITE OXIDATION ; TREATMENT PLANTS ; GRASSLAND SOILS
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Microbiology
WOS SubjectEcology ; Microbiology
WOS IDWOS:000295688500015
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/6544
Collection单细胞中心
Affiliation1.Chinese Acad Sci, Inst Soil Sci, State Key Lab Soil & Sustainable Agr, Nanjing 210008, Jiangsu Provinc, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Nanjing Agr Univ, Coll Resources & Environm Sci, Nanjing, Peoples R China
4.Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, BioEnergy Genome Ctr, Qingdao, Peoples R China
Recommended Citation
GB/T 7714
Xia, Weiwei,Zhang, Caixia,Zeng, Xiaowei,et al. Autotrophic growth of nitrifying community in an agricultural soil[J]. ISME JOURNAL,2011,5(7):1226-1236.
APA Xia, Weiwei.,Zhang, Caixia.,Zeng, Xiaowei.,Feng, Youzhi.,Weng, Jiahua.,...&Jia, Zhongjun.(2011).Autotrophic growth of nitrifying community in an agricultural soil.ISME JOURNAL,5(7),1226-1236.
MLA Xia, Weiwei,et al."Autotrophic growth of nitrifying community in an agricultural soil".ISME JOURNAL 5.7(2011):1226-1236.
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