| Characterization of D-xylonate dehydratase YjhG from Escherichia coli | |
Jiang, Yudong1,2; Liu, Wei1,2; Cheng, Tao1; Cao, Yujin1; Zhang, Rubing1,2; Xian, Mo1
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| 2015-07-01 | |
| 发表期刊 | BIOENGINEERED
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| 卷号 | 6期号:4页码:227-232 |
| 摘要 | D-xylonate dehydratase YjhG from Escherichia coli can convert D-xylonate into 2-keto-3-deoxy- D-xylonate (KDX), and is a key enzyme in the biosynthesis of 1,2,4-butanetriol and other chemicals. However, the biochemical properties of YjhG still remain unknown. In this study, the activity assay method for YjhG was established based on semicarbazide method, in which KDX reacts with semicarbazide reagent, and is further quantified by high-resolution mass spectrometry. The effect of reaction conditions on YjhG activity was determined in vitro using purified His-tagged YjhG protein. This enzyme showed maximal activity at 30 degrees C and pH 8.0. Bivalent metal ions such as Mg2+ and Mn2+ activated, whereas Ni2+ and Zn2+ inhibited the activity of YjhG. Under optimal conditions, the K-m and V-max values were 4.88 mM and 78.62 mu M l(-1)h(-1), respectively, when using D-xylonate as a substrate. Amino acids sequence alignments and catalytic properties analysis revealed that YjhG might be a member of IlvD/EDD family. Results obtained in this study may lay a foundation for further investigation on YjhG and will benefit its application in biosynthesis of related chemicals. |
| 文章类型 | Article |
| 关键词 | Biosynthesis D-xylonate Dehydratase Escherichia Coli Yjhg 1 2 4-butanetriol |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1080/21655979.2015.1040208 |
| 关键词[WOS] | D-XYLOSE ; METABOLISM ; ACID |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000358662300007 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/6521 |
| 专题 | 生物基材料组群 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Yudong,Liu, Wei,Cheng, Tao,et al. Characterization of D-xylonate dehydratase YjhG from Escherichia coli[J]. BIOENGINEERED,2015,6(4):227-232. |
| APA | Jiang, Yudong,Liu, Wei,Cheng, Tao,Cao, Yujin,Zhang, Rubing,&Xian, Mo.(2015).Characterization of D-xylonate dehydratase YjhG from Escherichia coli.BIOENGINEERED,6(4),227-232. |
| MLA | Jiang, Yudong,et al."Characterization of D-xylonate dehydratase YjhG from Escherichia coli".BIOENGINEERED 6.4(2015):227-232. |
| 条目包含的文件 | 条目无相关文件。 | |||||
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