Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation

doi:10.1038/srep12240

QIBEBT-IR > 资源植物与环境工程研究组

	Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation
	Tang, Xianfeng 1; Zhuang, Yamei 1,2; Qi, Guang 1; Wang, Dian 1; Liu, Huanhuan 1; Wang, Kairong 2; Chai, Guohua 1; Zhou, Gongke 1
	2015-07-16
发表期刊	SCIENTIFIC REPORTS
卷号	5
摘要	Wood is formed by the successive addition of secondary xylem, which consists of cells with a conspicuously thickened secondary wall composed mainly of cellulose, xylan and lignin. Currently, few transcription factors involved in the direct regulation of secondary wall biosynthesis have been characterized in tree species. Here, we show that PdMYB221, a poplar ortholog of the Arabidopsis R2R3-MYB transcription factor AtMYB4, directly regulates secondary wall biosynthesis during wood formation. PdMYB221 is predominantly expressed in cells of developing wood, and the protein it encodes localizes to the nucleus and acts as a transcriptional repressor. Ectopic expression of PdMYB221 resulted in reduced cell wall thicknesses of fibers and vessels in Arabidopsis inflorescence stems. The amounts of cellulose, xylose, and lignin were decreased and the expression of key genes synthesizing the three components was suppressed in PdMYB221 overexpression plants. Transcriptional activation assays showed that PdMYB221 repressed the promoters of poplar PdCESA7/8, PdGT47C, PdCOMT2 and PdCCR1. Electrophoretic mobility shift assays revealed that PdMYB221 bound directly to the PdCESA8, PdGT47C, and PdCOMT2 promoters. Together, our results suggest that PdMYB221 may be involved in the negative regulation of secondary wall formation through the direct and indirect suppression of the gene expression of secondary wall biosynthesis.
文章类型	Article
WOS标题词	Science & Technology
DOI	10.1038/srep12240
关键词[WOS]	MYB TRANSCRIPTION FACTOR ; FUNCTIONAL-CHARACTERIZATION ; ARABIDOPSIS ; POPULUS ; EXPRESSION ; LIGNIN ; FAMILY ; CELLULOSE ; TOBACCO ; XYLEM
收录类别	SCI
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000358009300001
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/6515
专题	资源植物与环境工程研究组
作者单位	1.Chinese Acad Sci, Key Lab Biofuels, Shandong Prov Key Lab Energy Genet, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Qingdao Agr Univ, Coll Resources & Environm, Qingdao Engn Res Ctr Rural Environm, Qingdao 266109, Peoples R China
推荐引用方式 GB/T 7714	Tang, Xianfeng,Zhuang, Yamei,Qi, Guang,et al. Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation[J]. SCIENTIFIC REPORTS,2015,5.
APA	Tang, Xianfeng.,Zhuang, Yamei.,Qi, Guang.,Wang, Dian.,Liu, Huanhuan.,...&Zhou, Gongke.(2015).Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation.SCIENTIFIC REPORTS,5.
MLA	Tang, Xianfeng,et al."Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation".SCIENTIFIC REPORTS 5(2015).