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Towards high-throughput microfluidic Raman-activated cell sorting
Zhang, Qiang1,2,3; Zhang, Peiran1,2; Gou, Honglei1,2; Mou, Chunbo3; Huang, Wei E.1,2,4; Yang, Menglong5,6; Xu, Jian1,2; Ma, Bo1,2
2015
Source PublicationANALYST
Volume140Issue:18Pages:6163-6174
Abstract

Raman-activated cell sorting (RACS) is a promising single-cell analysis technology that is able to identify and isolate individual cells of targeted type, state or environment from an isogenic population or complex consortium of cells, in a label-free and non-invasive manner. However, compared with those widely used yet labeling-required or staining-dependent cell sorting technologies such as FACS and MACS, the weak Raman signal greatly limits the further development of the existing RACS systems to achieve higher throughput. Strategies that can tackle this bottleneck include, first, improvement of Raman-acquisition efficiency and quality based on advanced Raman spectrometers and enhanced Raman techniques; second, development of novel microfluidic devices for cell sorting followed by integration into a complete RACS system. Exploiting these strategies, prototypes for a new generation of RACS have been demonstrated, such as flow-based OT-RACS, DEP-RACS, and SERS/CARS flow cytometry. Such high-throughput microfluidic RACS can provide biologists with a powerful single-cell analysis tool to explore the scientific questions or applications that have been beyond the reach of FACS and MACS.

SubtypeReview
WOS HeadingsScience & Technology ; Physical Sciences
DOI10.1039/c5an01074h
WOS KeywordSURFACE-ENHANCED RAMAN ; CIRCULATING TUMOR-CELLS ; SINGLE MICROBIAL-CELLS ; EMBRYONIC STEM-CELLS ; FREE FLOW-CYTOMETRY ; IN-VIVO ; LASER TWEEZERS ; LIVING CELLS ; LIVE CELLS ; SCATTERING MICROSCOPY
Indexed BySCI
Language英语
WOS Research AreaChemistry
WOS SubjectChemistry, Analytical
WOS IDWOS:000360648500001
Citation statistics
Cited Times:34[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/6498
Collection单细胞中心组群
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Single Cell Ctr, CAS Key Lab Biofuels, Qingdao, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao, Peoples R China
3.Qingdao Univ, Coll Chem Sci & Engn, Qingdao, Peoples R China
4.Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England
5.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Publ Lab, Qingdao, Peoples R China
6.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Qingdao, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Qiang,Zhang, Peiran,Gou, Honglei,et al. Towards high-throughput microfluidic Raman-activated cell sorting[J]. ANALYST,2015,140(18):6163-6174.
APA Zhang, Qiang.,Zhang, Peiran.,Gou, Honglei.,Mou, Chunbo.,Huang, Wei E..,...&Ma, Bo.(2015).Towards high-throughput microfluidic Raman-activated cell sorting.ANALYST,140(18),6163-6174.
MLA Zhang, Qiang,et al."Towards high-throughput microfluidic Raman-activated cell sorting".ANALYST 140.18(2015):6163-6174.
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