KMS Qingdao Institute of Biomass Energy and Bioprocess Technology ,CAS
Bare magnetic nanoparticles as fluorescence quenchers for detection of thrombin | |
Yu, Jiemiao1,2,3; Yang, Liangrong2; Liang, Xiangfeng2; Dong, Tingting2,3; Liu, Huizhou1,2 | |
2015 | |
发表期刊 | ANALYST |
卷号 | 140期号:12页码:4114-4120 |
摘要 | Rapid and sensitive detection of thrombin has very important significance in clinical diagnosis. In this work, bare magnetic iron oxide nanoparticles (magnetic nanoparticles) without any modification were used as fluorescence quenchers. In the absence of thrombin, a fluorescent dye (CY3) labeled thrombin aptamer (named CY3-aptamer) was adsorbed on the surface of magnetic nanoparticles through interaction between a phosphate backbone of the CY3-aptamer and hydroxyl groups on the bare magnetic nanoparticles in binding solution, leading to fluorescence quenching. Once thrombin was introduced, the CY3-aptamer formed a G-quartet structure and combined with thrombin, which resulted in the CY3-aptamer being separated from the magnetic nanoparticles and restoration of fluorescence. This proposed assay took advantage of binding affinity between the CY3-aptamer and thrombin for specificity, and bare magnetic nanoparticles for fluorescence quenching. The fluorescence signal had a good linear relationship with thrombin concentration in the range of 1-60 nM, and the limit of detection for thrombin was estimated as low as 0.5 nM. Furthermore, this method could be applied for other target detection using the corresponding fluorescence labeled aptamer. |
文章类型 | Article |
WOS标题词 | Science & Technology ; Physical Sciences |
DOI | 10.1039/c5an00519a |
关键词[WOS] | HOST-GUEST-RECOGNITION ; SINGLE-STRANDED-DNA ; ELECTROCHEMICAL APTASENSOR ; MASS-SPECTROMETRY ; GOLD NANOPARTICLES ; ULTRASENSITIVE DETECTION ; SIGNAL AMPLIFICATION ; OXIDE NANOPARTICLES ; SENSITIVE DETECTION ; GRAPHENE OXIDE |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Chemistry |
WOS类目 | Chemistry, Analytical |
WOS记录号 | WOS:000355852400030 |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qibebt.ac.cn/handle/337004/6474 |
专题 | 资源环境与绿色分离研究组 |
作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Key Lab Green Proc & Engn, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Yu, Jiemiao,Yang, Liangrong,Liang, Xiangfeng,et al. Bare magnetic nanoparticles as fluorescence quenchers for detection of thrombin[J]. ANALYST,2015,140(12):4114-4120. |
APA | Yu, Jiemiao,Yang, Liangrong,Liang, Xiangfeng,Dong, Tingting,&Liu, Huizhou.(2015).Bare magnetic nanoparticles as fluorescence quenchers for detection of thrombin.ANALYST,140(12),4114-4120. |
MLA | Yu, Jiemiao,et al."Bare magnetic nanoparticles as fluorescence quenchers for detection of thrombin".ANALYST 140.12(2015):4114-4120. |
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