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Biosynthesis of poly(3-hydroxypropionate) from glycerol using engineered Klebsiella pneumoniae strain without vitamin B-12
Feng, Xinjun1,2; Xian, Mo1; Liu, Wei1; Xu, Chao1; Zhang, Haibo1; Zhao, Guang1
2015-03-01
发表期刊BIOENGINEERED
卷号6期号:2页码:77-81
摘要

Poly(3-hydroxypropionate) (P3HP) is a biodegradable and biocompatible thermoplastic. Previous studies demonstrated that engineered Escherichia coli strains can produce P3HP with supplementation of expensive vitamin B-12. The present study examined the production of P3HP from glycerol in the recombinant Klebsiella pneumoniae strain, which naturally synthesizes vitamin B-12. The genes glycerol dehydratase and its reactivation factor (dhaB123, gdrA, and gdrB from K. pneumoniae), aldehyde dehydrogenase (aldH from E. coli) were cloned and expressed in K. pneumoniae to produce 3-hydroxypropionate (3HP), with 2 genes (dhaT and yqhD) for biosynthesis of 1,3-propanediol were deleted. To obtain P3HP production, propionyl-CoA synthetase (prpE from E. coli) and polyhydroxyalkanoate synthase (phaC from Ralstonia eutropha) were introduced. Under the appropriate aeration condition, the cell yield and P3HP content were 0.24g/L and 12.7% (wt/wt [cell dry weight]) respectively along with 2.03g/L 3HP after 48h cultivation. Although the yield is relatively low, this study shows the feasibility of producing P3HP in K. pneumoniae from glycerol without vitamin B-12 for the first time. The results also suggest that the aeration conditions should be optimized carefully for the efficient production of P3HP.

文章类型Article
关键词Glycerol Klebsiella Pneumoniae Poly(3-hydroxypropionate) Vitamin B-12 3-hydroxypropionate
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1080/21655979.2015.1011027
关键词[WOS]RECOMBINANT ESCHERICHIA-COLI ; 3-HYDROXYPROPIONIC ACID ; MICROBIAL SYNTHESIS ; PATHWAY ; 1,3-PROPANEDIOL ; DEHYDROGENASE ; METABOLISM
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000353946700004
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/6441
专题生物基材料组群
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Feng, Xinjun,Xian, Mo,Liu, Wei,et al. Biosynthesis of poly(3-hydroxypropionate) from glycerol using engineered Klebsiella pneumoniae strain without vitamin B-12[J]. BIOENGINEERED,2015,6(2):77-81.
APA Feng, Xinjun,Xian, Mo,Liu, Wei,Xu, Chao,Zhang, Haibo,&Zhao, Guang.(2015).Biosynthesis of poly(3-hydroxypropionate) from glycerol using engineered Klebsiella pneumoniae strain without vitamin B-12.BIOENGINEERED,6(2),77-81.
MLA Feng, Xinjun,et al."Biosynthesis of poly(3-hydroxypropionate) from glycerol using engineered Klebsiella pneumoniae strain without vitamin B-12".BIOENGINEERED 6.2(2015):77-81.
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